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  • Original Article
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Graft-Versus-Host Disease

The role of HLA–G 14-bp polymorphism in allo-HSCT after short-term course MTX for GvHD prophylaxis

Bone Marrow Transplantation volume 47, pages 120–124 (2012)Cite this article

Abstract

HLA-G molecules are HLA class Ib antigens characterized by tolerogenic and immunoinhibitory functions. The HLA-G 14-bp insertion/deletion (ins/del) polymorphism controls protein expression and seems to be implicated in both MTX treatment response and SCT outcome. The aim of our study is to evaluate the role of HLA-G 14 bp polymorphism in subjects affected by hematological malignancies undergoing allo-SCT and receiving MTX therapy for GvHD prophylaxis. We performed a retrospective analysis of HLA-G 14 bp polymorphism using a specific PCR in 47 recipients and in their respective donors, and evaluated the correlation with the incidence of aGvHD, OS and disease-free survival (DFS) after allo-SCT. We did not observe any correlation between this polymorphism and the risk of aGvHD occurrence. On the contrary, we found that the recipients with a 14 bp ins/14 bp ins genotype were characterized by a lower OS and DFS in univariate and multivariate analysis (OS=OR: 3.235; DFS=OR: 3.302). These data indicate a role for recipient HLA-G 14 bp polymorphism in allo-SCT immunotolerance status and follow-up.

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Authors and Affiliations

  1. Hematology Department, Istituto di Ematologia, Università Cattolica del Sacro Cuore, Rome, Italy

    P Chiusolo, S Bellesi, N Piccirillo, S Giammarco, S Marietti, D De Ritis, E Metafuni, S Sica & G Leone

  2. Department of Experimental and Diagnostic Medicine, Section of Medical Genetics, University of Ferrara, Ferrara, Italy

    M Stignani, O R Baricordi & R Rizzo

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  1. P Chiusolo
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  2. S Bellesi
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  3. N Piccirillo
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Correspondence to P Chiusolo.

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Chiusolo, P., Bellesi, S., Piccirillo, N. et al. The role of HLA–G 14-bp polymorphism in allo-HSCT after short-term course MTX for GvHD prophylaxis. Bone Marrow Transplant 47, 120–124 (2012). https://doi.org/10.1038/bmt.2011.40

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