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Pre-clinical Studies

Survival time of cardiac allografts prolonged by isogeneic BMT in mice

Abstract

To find an approach to prolong the survival time of cardiac allografts in a BALB/c-to-C57/BL6 heterotopic heart transplant model and to try to figure out related chemokines and cytokines, isogeneic and allogeneic BM cells were obtained from pregnant C57/BL6 (♀C57/BL6 × ♂BALB/c) and regular BALB/c mice and injected to the half lethally irradiated C57/BL6 mice 1 day before heart transplantation. Recipients were treated with CsA or phosphate-buffered saline for 7 days. Isogeneic BMT (iBMT) from pregnant C57/BL6 mice was observed to significantly prolong the survival of BALB/c allografts and reduce the lymphocyte infiltration. Allogeneic BMT (aBMT) and iBMT both exhibited signicantly less T-cell proliferation reactivity and the similar degree of chimerism. There was no significant difference in these groups of IFN-γ and IL-4 production. The level of chemokine MIG (CXCL9) dramatically decreased in aBMT and iBMT groups compared with the control group. But there were no significant differences between aBMT and iBMT group. IL-17 and RORγ(t) (receptor-related orphan receptor) production were downregulated in iBMT recipients. These results indicate that iBMT can prolong the survival of cardiac allografts. IL-17 production downregulated in iBMT recipients. This means that iBMT may have important therapeutic implications.

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Acknowledgements

This work was supported by the Kidney Disease Center, the First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, China and the Kidney Disease Immunology Laboratory, the third grade laboratory, state administration of traditional Chinese medicine of the people's republic of China.

Author contributions: Zhimin Chen participated in research design, performance of the research, data analysis and writing of the manuscript; Hong Jiang participated in research design; Rongjun Chen participated in performance of the research; Shi Feng participated in performance of the research; Juan Jin participated in performance of the research; Yan Bi participated in performance of the research; Hao Yang contributed in the writing of the manuscript; Jianghua Chen participated in research design, data analysis and writing of the manuscript.

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Correspondence to J Chen.

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Chen, Z., Jiang, H., Chen, R. et al. Survival time of cardiac allografts prolonged by isogeneic BMT in mice. Bone Marrow Transplant 47, 1118–1125 (2012). https://doi.org/10.1038/bmt.2011.215

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