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Treatment of a severe extensive cutaneous chronic GVHD after allo-HSCT using glycerolyzed skin allografts and cultured epidermis from the same donor

Bone Marrow Transplantation volume 46, pages 1153–1155 (2011)Cite this article

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The first stage of skin reconstruction was to transplant 1.5 meshed allogeneic glycerolyzed skin to ensure a proper dermal bed for the following transplantation of cultured keratinocytes (Figure 1b).

After obtaining informed consent from the sister, a 5 cm2 full-thickness skin biopsy was harvested. Keratinocytes were isolated and then cultured, as previously described,1 for 3 weeks. Passage 2 and 3 keratinocytes were grafted after dispase treatment and clipping on Vaseline gauzes as support for transfer onto the patient's back2 (Figure 1c). Biweekly dressings under general anesthesia were performed, alternating antiseptic dressings and graft of cultured epidermis.

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Figure 1
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References

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Authors and Affiliations

  1. Hematology Department, Edouard Herriot Hospital, Lyon, France

    R Crocchiolo, F-E Nicolini, M Sobh, S Ducastelle-Lepretre, H Labussiere & M Michallet

  2. Histocompatibility Laboratory and HLA typing, Etablissement Francais du Sang, Lyon, France

    V Dubois

  3. Banque de Tissus et de cellules, Edouard Herriot Hospital, Lyon, France

    C Auxenfans & O Damour

  4. Plastic Surgery Department, Edouard Herriot Hospital, Lyon, France

    A Mojallal & F Braye

Authors
  1. R Crocchiolo
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  2. F-E Nicolini
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  3. M Sobh
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  4. S Ducastelle-Lepretre
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  5. H Labussiere
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  6. V Dubois
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  7. C Auxenfans
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  8. A Mojallal
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  9. O Damour
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  10. F Braye
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  11. M Michallet
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Corresponding author

Correspondence to M Michallet.

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The authors declare no conflict of interest.

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Crocchiolo, R., Nicolini, FE., Sobh, M. et al. Treatment of a severe extensive cutaneous chronic GVHD after allo-HSCT using glycerolyzed skin allografts and cultured epidermis from the same donor. Bone Marrow Transplant 46, 1153–1155 (2011). https://doi.org/10.1038/bmt.2010.247

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  • DOI: https://doi.org/10.1038/bmt.2010.247

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