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Transplant Technology

Hexaminolevulinate-mediated photodynamic purging of leukemia cells from BM

Bone Marrow Transplantation volume 45, pages 1553–1561 (2010)Cite this article

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Abstract

Photodynamic therapy (PDT) with porphyrin precursors has been established for tumor treatment. This study aimed at examining applicability of hexaminolevulinate (HAL) for photodynamic purging of leukemic cells from BM grafts and evaluating the clinical relevance of in vitro models. The PDT dose resulting in no colony formation by leukemic cells in vitro, in pure form or in a mixture with BM cells, was insufficient for complete killing of the leukemic cells ex vivo and for the treatment of the leukemia-bearing animals in vivo. The efficacy of HAL–PDT in cell lines in vitro should be verified in clinically relevant in vivo models.

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Acknowledgements

We are grateful to PhotoCure ASA for providing HAL, Dr Anne Tierens and Dr Meng Yu Wang for valuable discussions; Marie-Therese R Strand, Marianne Sand Dyrhaug and Vladimir Iani for technical assistance. Beata Čunderlíková is grateful for grant supports from The Research Council of Norway (project 172818) and The Norwegian Radium Hospital Research Foundation (project 0609).

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Authors and Affiliations

  1. Department of Pathology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

    B Čunderlíková, V Vasovič, J M Nesland & Q Peng

  2. International Laser Centre, Bratislava, Slovakia

    B Čunderlíková

  3. Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, USA

    F Sieber

  4. Department of Medical Physics, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway

    T Furre

  5. Faculty Division, Norwegian Radium Hospital, Medical Faculty, University of Oslo, Oslo, Norway

    J M Nesland

  6. State Key Laboratory for Advanced Photonic Materials and Devices, Fudan University, Shanghai, PR China

    Q Peng

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  1. B Čunderlíková
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  4. T Furre
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Correspondence to Q Peng.

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Čunderlíková, B., Vasovič, V., Sieber, F. et al. Hexaminolevulinate-mediated photodynamic purging of leukemia cells from BM. Bone Marrow Transplant 45, 1553–1561 (2010). https://doi.org/10.1038/bmt.2009.382

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  • DOI: https://doi.org/10.1038/bmt.2009.382

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