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Post-Transplant Events

Relationship between IgG titers and opsonocytophagocytic activity of anti-pneumococcal antibodies after immunization with the 7-valent conjugate vaccine in allogeneic stem cell transplant

Abstract

The European Group for Blood and Marrow Transplantation has recently run a prospective, randomized trial comparing an early (3 months) vs a late (9 months) immunization program after allo-SCT with three doses of the conjugate 7-valent pneumococcal vaccine. This trial has shown that the response rate assessed 1 month after the third dose of conjugate vaccine was not inferior after an early vaccination vs a late vaccination. Part of the responder cohort of these patients (n=28) were chosen to assess the functionality of the anti-pneumococcal antibodies through an opsonophagocytic assay, 1 month after the third dose of conjugate vaccine. We have assessed the relationship between IgG titers measured by the pneumococcal ELISA and functional titers measured by opsonophagocytic assay of anti-pneumococcal antibodies. We found a significant correlation between titers for both assays, and conclude that the response to PCV7 after SCT induces functional antibodies.

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Acknowledgements

The study was funded by grants from the EBMT (data management, statistical analysis) and from Wyeth-Pharmaceuticals (provision and delivery of the vaccines, serum sample collection and antibody assays). We thank the investigators of the IDWP01 trial: Patricia Ribaud, Rafael de la Camara, Rodrigo Martino, Andrew Ullmann, Terttu Parkkali, Anna Locasciulli, Karima Yakouben, Karlis Pauksens, Dietger Niederwieser and Jane Apperley. We are indebted to the members of the Safety Committee of the IDWP01 trial: Dan Engelhard, MD, Hadassah University Hospital, Israel; Pierre Reusser, MD, Porrentruy, Switzerland and Philippe Reinert Créteil, France. We are grateful to Eric Bonnet, MD, Clinical Trial Coordinator of the study at Wyeth Pharmaceuticals, and to all the pharmacists, data managers and nurses of the study transplant centers for their participation in the study. We thank Branda Hu, at the Wyeth US laboratory, for performing the ELISA tests, as well as Kim Champion and the staff of the EBMT London Office for randomizing the patients.

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Correspondence to C Cordonnier.

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Eric Bonnet is an employee of Wyeth-Pharma, France and the Wyeth study coordinator of the IDWP01 trial. Kathrin U Jansen and Michael Pride are employees of Wyeth and were coordinating the serological OPA testing. None of the other authors has any conflict of interest to declare.

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The results of this study have been orally presented at the Infectious Diseases Working Party session of the 35th Annual Meeting of the European Group for Blood and Marrow Transplantation, Göteborg, Sweden, March 31, 2009.

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Cordonnier, C., Labopin, M., Jansen, K. et al. Relationship between IgG titers and opsonocytophagocytic activity of anti-pneumococcal antibodies after immunization with the 7-valent conjugate vaccine in allogeneic stem cell transplant. Bone Marrow Transplant 45, 1423–1426 (2010). https://doi.org/10.1038/bmt.2009.364

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