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Long-term remission after CD34+-selected PBSCT in a patient with advanced intra-abdominal desmoplastic small round-cell tumor

Bone Marrow Transplantation volume 45, pages 793–795 (2010)Cite this article

Furthermore, we performed immunohistochemical expression analysis with a large panel of MoAbs directed against tumor-associated antigens, such as antigens of the Cancer–Testis (C/T) gene family that are frequently expressed in some types of sarcoma6 and neuroblastoma, possibly acting as immunological target antigens.7 However, none of them were expressed in the patient's tumor specimen.

In our patient, using reverse transcription-PCR, the unselected leukapheresis product revealed contaminating tumor cells. This is in accordance with earlier reports that, in IADSRCT, the characteristic t(11;22) translocation is frequently present in peripheral blood and BM cells.2 After CD34+ selection, tumor cell contamination might have been markedly reduced, although in Ewing sarcoma (ES) patients, the role of CD34+ selection has been controversially discussed, as the CD34 expression can be occasionally detected in ES cells, bearing the potential risk that tumor cells are even enriched after CD34+ selection. Not having backup aliquots of CD34+-selected PBSCs of our patient, we could not analyze tumor cell contamination of CD34+-PBSCs, but as the CD34 Ag was not expressed in the IADSRCT specimen itself, this makes tumor cell enrichment after CD34+ selection highly unlikely.

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References

  1. Gerald WL, Rosai J, Ladanyi M . Characterization of the genomic breakpoint and chimeric transcripts in the EWS-WT1 gene fusion of desmoplastic small round cell tumor. Proc Natl Acad Sci USA 1995; 92: 1028–1032.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Bertuzzi A, Castagna L, Nozza A, Quagliuolo V, Siracusano L, Balzarotti M et al. High-dose chemotherapy in poor-prognosis adult small round-cell tumors: clinical and molecular results from a prospective study. J Clin Oncol 2002; 20: 2181–2188.

    Article  CAS  PubMed  Google Scholar 

  3. Worley BS, van den Broeke LT, Goletz TJ, Pendleton CD, Daschbach EM, Thomas E K et al. Antigenicity of fusion proteins from sarcoma-associated chromosomal translocations. Cancer Res 2001; 61: 6868–6875.

    CAS  PubMed  Google Scholar 

  4. Bertuzzi A, Castagna L, Quagliuolo V, Ginanni V, Compasso S, Magagnoli M et al. Prospective study of high-dose chemotherapy and autologous peripheral stem cell transplantation in adult patients with advanced desmoplastic small round-cell tumour. Br J Cancer 2003; 89: 1159–1161.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Jantzer P, Schendel DJ . Human renal cell carcinoma antigen-specific CTLs: antigen-driven selection and long-term persistence in vivo. Cancer Res 1998; 58: 3078–3086.

    CAS  PubMed  Google Scholar 

  6. Antonescu CR, Busam KJ, Iversen K, Kolb D, Coplan K, Spagnoli GC et al. MAGE antigen expression in monophasic and biphasic synovial sarcoma. Hum Pathol 2002; 33: 225–229.

    Article  PubMed  Google Scholar 

  7. Rodolfo M, Luksch R, Stockert E, Chen YT, Collini P, Ranzani T et al. Antigen-specific immunity in neuroblastoma patients: antibody and T-cell recognition of NY-ESO-1 tumor antigen. Cancer Res 2003; 63: 6948–6955.

    CAS  PubMed  Google Scholar 

  8. Engelhardt M, Douville J, Behringer D, Jahne A, Smith A, Henschler R et al. Hematopoietic recovery of ex vivo perfusion culture expanded bone marrow and unexpanded peripheral blood progenitors after myeloablative chemotherapy. Bone Marrow Transplant 2001; 27: 249–259.

    Article  CAS  PubMed  Google Scholar 

  9. Rill DR, Santana VM, Roberts WM, Nilson T, Bowman LC, Krance RA et al. Direct demonstration that autologous bone marrow transplantation for solid tumors can return a multiplicity of tumorigenic cells. Blood 1994; 84: 380–383.

    CAS  PubMed  Google Scholar 

  10. Adamson PC, Blaney SM, Widemann BC, Kitchen B, Murphy RF, Hannah AL et al. Pediatric phase I trial and pharmacokinetic study of the platelet-derived growth factor (PDGF) receptor pathway inhibitor SU101. Cancer Chemother Pharmacol 2004; 53: 482–488.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

Part of this work was supported by grants from the Deutsche Krebshilfe (Project-Nr. 106225 to LH and IM. We thank Professor Terracciano, Institute of Pathology, Basel, Switzerland for expert FISH analysis.

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Author notes

  1. B Rumstadt and C F Waller: These authors contributed equally to this paper

Authors and Affiliations

  1. Department of Hematology/Oncology, Freiburg University Medical Center, Freiburg, Germany

    L Houet, I Möller, M Engelhardt, D Herchenbach, M Schnitzler, R Mertelsmann & C F Waller

  2. Gerhard-Domagk-Institute of Pathology, Universityof Muenster, Muenster, Germany

    G Köhler & H Schmidt

  3. Department of Pathology, Freiburg University Medical Center, Freiburg, Germany

    A Schmitt-Graeff

  4. Ludwig Institute for Cancer Research, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    A A Jungbluth

  5. Department of Surgery, Diakoniekrankenhaus Mannheim, Mannheim, Germany

    B Rumstadt

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  1. L Houet
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Correspondence to C F Waller.

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Houet, L., Möller, I., Engelhardt, M. et al. Long-term remission after CD34+-selected PBSCT in a patient with advanced intra-abdominal desmoplastic small round-cell tumor. Bone Marrow Transplant 45, 793–795 (2010). https://doi.org/10.1038/bmt.2009.226

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