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Graft-Versus-Tumor Effects

Cutaneous lymphocyte antigen-positive T cells may predict the development of acute GVHD: alterations and differences of CLA+ T- and NK-cell fractions

Bone Marrow Transplantation volume 43, pages 863–873 (2009)Cite this article

Abstract

Acute GVHD (aGVHD) is a serious complication after allogeneic SCT (allo-SCT). However, an adequate immunological index is not yet available for assessing its severity. We analyzed the fraction of cutaneous lymphocyte antigen (CLA)+ cells in peripheral blood T and natural killer (NK) cells in 33 patients and evaluated its association with aGVHD. The CLA+ T-cell fraction often increased 3–7 days before the onset of aGVHD, and the maximum percentage of CLA+ T cells in grades II–IV aGVHD cases was significantly higher than that in grade 0 or I aGVHD (P<0.01). When the cutoff value of the maximum CLA+ T-cell percentage was set at 20%, any higher percentage was a significant risk for the development of severe aGVHD (P<0.0001). The maximum CLA+ T-cell percentage was significantly correlated with a high body temperature, low percutaneous oxygen saturation, and fibrinogen/fibrin degradation product D-dimer level. The post-allo-SCT CLA+ T cells exhibited a high ability to produce IL-2 and IFN-γ, and may be the effectors and immunological markers for aGVHD. The CLA+ NK-cell-fraction steadily increased 2–4 weeks after allo-SCT but was not influenced by aGVHD. The CLA+ T-cell percentage may predict the development of severe aGVHD in clinical settings.

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Acknowledgements

We thank the medical and nursing staff of Niigata University Medical and Dental Hospital for their help in patient care; the laboratory staff for examining the clinical samples; Ms M Sakaue and Ms H Itoh for their technical assistance; Dr R Nishiuchi (Department of Pediatrics, Okayama University Graduate School of Medical and Dental Sciences), Dr T Teshima, Dr M Harada (Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences), for their valuable advice.

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Authors and Affiliations

  1. Department of Internal Medicine, Division of Hematology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    J Tsuchiyama, T Furukawa, I Fuse & Y Aizawa

  2. Department of Pathology, Kawasaki Medical School, Okayama, Japan

    J Tsuchiyama

  3. Department of Pathology, Okayama University Graduate School of Medical and Dental Sciences, Okayama, Japan

    T Yoshino

  4. Department of Anesthesiology, Okayama University Graduate School of Medical and Dental Sciences, Okayama, Japan

    T Saito

  5. Department of Dermatology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    K Ito

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  1. J Tsuchiyama
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Correspondence to J Tsuchiyama.

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Tsuchiyama, J., Yoshino, T., Saito, T. et al. Cutaneous lymphocyte antigen-positive T cells may predict the development of acute GVHD: alterations and differences of CLA+ T- and NK-cell fractions. Bone Marrow Transplant 43, 863–873 (2009). https://doi.org/10.1038/bmt.2008.392

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