Abstract
Neuronal nicotinic acetylcholine receptors (nAChRs) are the superfamily of ligand-gated ion channels and widely expressed throughout the central and peripheral nervous systems. nAChRs play crucial roles in modulating a wide range of higher cognitive functions by mediating presynaptic, postsynaptic, and extrasynaptic signaling. Thus far, nine alpha (α2-α10) and three beta (β2, β3, and β4) subunits have been identified in the CNS, and these subunits assemble to form a diversity of functional nAChRs. Although α4β2- and α7-nAChRs are the two major functional nAChR types in the CNS, α6*-nAChRs are abundantly expressed in the midbrain dopaminergic (DAergic) system, including mesocorticolimbic and nigrostriatal pathways, and particularly present in presynaptic nerve terminals. Recently, functional and pharmacological profiles of α6*-nAChRs have been assessed with the use of α6 subunit blockers such as α-conotoxin MII and PIA, and also by using α6 subunit knockout mice. By modulating DA release in the nucleus accumbens (NAc) and modulating GABA release onto DAergic neurons in the ventral tegmental area (VTA), α6*-nAChRs may play important roles in the mediation of nicotine reward and addiction. Furthermore, α6*-nAChRs in the nigrostriatal DAergic system may be promising targets for selective preventative treatment of Parkinson's disease (PD). Thus, α6*-nAChRs may hold promise for future clinical treatment of human disorders, such as nicotine addiction and PD. In this review, we mainly focus on the recent advances in the understanding of α6*-nAChR function, pharmacology and pathophysiology.
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Introduction
Neuronal nicotinic acetylcholine receptors (nAChRs) belong to the ligand-gated ion channel superfamily, which include GABAA, glycine, and 5-HT3 receptors1. nAChRs are widely distributed throughout the central nervous system (CNS) and activation of various nAChRs may play important roles in regulation of higher cognitive functions2. nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits, which can be classified as alpha subunits, containing two cysteine residues at positions analogous to Cys192 and Cys193, and non-alpha subunits ('structural' subunits), which can be defined as beta subunits when they are expressed in the vertebrate nervous system3, 4. To date, nine alpha subunits (α2–α10) and three beta subunits (β2, β3, and β4) have been identified in the CNS5. A single subunit is about 600 amino acids long and has four separate transmembrane segments (TM1-TM4) with a large N- and a small C-termini facing the synaptic cleft6, 7. Studies using affinity labeling and mutagenesis suggest that ligand-binding sites are located at the interfaces of the N-terminal hydrophilic domain of α subunit and its adjacent α/β subunit8, and the wall of the ionic pore is formed by the second hydrophobic transmembrane segment (TM2) of each subunit6, 7. The cation channel (mainly permeable to Na+ and Ca2+) can be opened only when the receptor is activated by endogenous acetylcholine (ACh) or exogenous ligand (eg nicotine) binding to the binding site (Figure 1)7. Furthermore, the β subunits also largely contribute to the physiological and pharmacological properties (such as desensitization, inward rectification, and functional rundown) of the receptors9, 10.
Physiological and pharmacological profiles of nAChRs range widely, depending on subunit co-assembly. nAChRs can be divided into two subfamilies, homomeric nAChRs (native α7 or heterologously expressed α7–9 subunits) and heteromeric nAChRs (α2–6 subunits combined with β subunits)8, 11. Although there are many possible combinations of neuronal α and β subunits, the majority of functional heteromeric nAChRs expressed throughout the brain are α4β2-containing nAChRs (α4β2*-nAChRs, *indicates the presence of possible additional subunits)12. Though α6*-nAChRs were characterized in the early 1990′s13, 14, it was not reported that α6 subunit could form functional heteromeric nAChRs until 199715. Immunoprecipitation experiments demonstrated that not only α4β2-nAChRs, but also heteromeric α6*-nAChRs (ie, α6β2- and α4α6β2-nAChRs) are highly expressed in mesolimbic DAergic system16. More importantly, α6*-nAChRs expressed on DAergic neurons can be activated by endogenous ACh or exogenous nicotine and analogs, which suggest that the activation of α6*-nAChRs may play vital roles in central cholinergic circuits, including modulation of locomotor behaviour and drug addiction17, 18. In addition, α6*-nAChRs are particularly susceptible to nigrostriatal damage, which may lead to Parkinson's disease (PD)19, 20. Accumulating evidence suggests that α6*-nAChRs might represent as potential therapeutic targets for treatment of PD and addictive behaviors18, 19, 20, 21.
Although α6*-nAChRs are abundant in the midbrain, they have been studied to a lesser extent than α4β2-nAChRs or α7-nAChRs22. In fact, we are only beginning to understand α6*-nAChR distribution, physiology and pharmacology, and the roles of these receptors in various diseases. In this review, we focus on recent advances in the understanding of α6*-nAChRs.
Anatomical distribution of α6*-nAChRs
Overwhelming experimental evidence demonstrates that neuronal nAChRs are present in a variety of regions of the brain, but the situation is much different for α6*-nAChRs, which are not abundantly expressed in the whole brain, but only detected in a restricted number of brain areas23, 24, 25. For example, in order to detect α6 subunit mRNA distribution in the CNS, Le Novere and colleagues explored the telencephalon, diencephalon, mesencephalon, and rhombencephalon of adult rat brain using in situ hybridization23 and found that the amount of α6 subunit mRNA is particularly high in several catecholaminergic nuclei, including locus coeruleus, ventral tegmental area (VTA) and substantia nigra (SN). In reticular thalamic nucleus, supramammillary nucleus, interpeduncular nucleus, medial and lateral habenula, and mesencephalic V nucleus, α6 subunit mRNA can be detected, but at lower levels, while no detectable α6 subunit mRNA labeling is observed in the anterior pretectal area23. Based on these data, authors concluded that α6*-nAChRs are the primary α subunit expressed in DAergic cell groups within the midbrain23. After this initial report, subsequent studies confirmed that α6*-nAChRs are highly expressed in the SN and VTA, and particularly expressed on most midbrain DAergic neurons rather than on non-DAergic neurons, either by applying single-cell reverse transcription polymerase chain reaction (RT-PCR) and patch-clamp recording in slices from rats, wild-type mice and α6 subunit null mutant mice25 or using double-labeling in situ hybridization in rats24. Additional in situ hybridization experiments using specific probes and stringent hybridization conditions demonstrated that α6 subunit mRNA is also abundantly expressed in neuroretina26. Other studies using [125I]α-CTX MII binding indicate that high levels of α6*-nAChRs are expressed in the visual system, including retina, optic tract, and its terminal fields, including geniculate nucleus, zonal and superficial gray layer, and olivary pretectal nucleus27. Although nAChRs are widely distributed in the peripheral nervous system (PNS)28, no α6 subunit mRNA has been detected in the PNS (ciliary, superior cervical, sympathetic, dorsal root, nodose and petrous ganglia), except in trigeminal nucleus and trigeminal ganglion26, 29. Thus, we can draw the conclusion that the natural expression of α6*-nAChRs appears to be largely excluded from the PNS and mainly restricted to the CNS, and particularly enriched in midbrain catecholaminergic nuclei.
Neuronal nAChRs are located postsynaptically on the cell-body, where they mediate direct postsynaptic effects and/or regulate firing patterns of DAergic neurons30, or presynaptically/preterminally on nerve terminals16, 22, 31, where they modulate neurotransmitter release5, 32, 33, 34, 35. Immunoprecipitation experiments have found that α6*-nAChRs account for 30% of 3H-Epibatidine (Epi) binding sites in striatum but only 5% in SN/VTA16. Furthermore, quantitative immunoprecipitation experiments have shown that most of α6*-nAChRs (87%) disappeared in 6-hydroxydopamine lesioned (6-OH DA) striatum36, further demonstrating the regulatory effects of presynaptic α6*-nAChRs on DA release37, 38. These results indicate that α6*-nAChRs appear to be preferentially addressed to DAergic nerve terminal compartments, since the majority of DAergic neurons in the SN project to the striatum16, 36. Our recent results using RT-PCR and patch clamp recordings in freshly dissociated VTA DAergic neurons22 are in good agreement with these observations. Tissue RT-PCR data showed that nAChR α6 subunit mRNA levels are >20-fold higher in the VTA than that of other subunits22, suggesting that α6*-nAChRs are mainly concentrated in midbrain catecholaminergic nuclei. However, 100 nmol/L α-conotoxin MII (α-CTX MII), an α6/α3-nAChR subtype-selective antagonist, had no significant effect on ACh (1mmol/L) induced postsynaptic inward currents on all three subtypes of nAChRs on VTA DAergic neurons22, suggesting that there are likely no functional α6*-nAChRs expressed on VTA DAergic neuronal somata under physiological conditions. Importantly, α-CTX MII (100 nmol/L) inhibited GABAergic spontaneous inhibitory postsynaptic currents in DAergic neurons containing GABAergic presynaptic boutons that were mechanically dissociated from the VTA22, implicating that most of the functional α6*-nAChRs are located on presynaptic structures rather than on somata of DAergic neurons in the VTA, or that functional α6*-nAChRs are expressed on somatodendrites of VTA DAergic neurons under the natural conditions, but the expression level is too low to be detected using patch-clamp recording. One recent paper supports this hypothesis, which shows that after genetic enhancement of α6*-nAChR expression, the function of α6*-nAChRs can be clearly tested using whole-cell recording technique39.
Therefore, nAChR α6 subunit mRNA is specifically expressed in DAergic neurons in the VTA and SN, and functional α6*-nAChRs are preferentially located on presynaptic nerve terminals.
Subunit composition of functional α6*-nAChRs
Studies using single-cell RT-PCR and patch-clamp recordings demonstrated that eight nAChR subunits (α3–7 and β2–4) are expressed on DAergic neurons in the VTA and SN25, and the combination of some these subunits with α6 subunit can form several subtypes of nAChRs. One of them possesses a putative α4α6α5(β2)2 composition since the whole cell currents mediated by this kind of nAChR can be inhibited by both DHβE and α-CTX MII25. Immunoprecipitation results from the same group found that α4, α6, and β2 are the most abundant nAChR subunits in the striatum16. Thus, it is reasonable to believe that the composition of naturally expressed functional α6*-nAChRs is very complex.
As early as 1982, researchers began to study nAChRs heterologously expressed in Xenopus oocytes and demonstrated that functional nAChRs could be inserted in the oocyte membrane and that activity of functional nAChRs could be measured using voltage clamp recording40, 41. Since then, Xenopus oocytes have become one of the most practical and widely used systems to express and study the physiological and pharmacological properties of nAChRs9, 15, 42, 43, 44, 45, 46. It is quite difficult, however, to express functional α6*-nAChRs in vitro. Fifteen years later, Gerzanich and co-workers found that α6 may form detectable functional α6*-nAChRs when chicken α6 subunit is expressed together with human β4 subunit15. This was the first in vitro synthesized functional α6*-nAChRs, proving that the α6 is not the so called “orphan” subunit. Kuryatov et al have tested even more complex mixtures of α6 with several other nAChR subunits and found that the coexpression of α6, β4, and β3 subunit can produce the most efficient α6*-nAChRs with the largest and most consistent responses44. Meanwhile, a complex variety of functional α6*-nAChRs, including α6α3β2-nAChRs, α6α4β2-nAChRs, α6β2β4-nAChRs, α6β2α5-nAChRs, α6β4β3α5-nAChRs, α6β4-nAChRs, and chimeric α6/α3β2β3-nAChRs and α6/α4β2β3-nAChRs were synthesized43, 44, 45, 46. But cotransfections of α6 and α3 without a β subunit can not yield functional receptors26. Interestingly, α6 subunit cotransfected with β2 and(or) β3 subunit either can form nAChR without gated ion channels thus can not further yield functional α6β2- or α6β2β3-nAChRs in Xenopus oocytes15, 44, or can form α6β2-nAChRs with very poor function in transfected cell line26, even though these subunits can form different ligand binding sites with high affinity for Epi44. Thus far, i n vitro synthesized functional α6*-nAChRs are very valuable preparations for development and testing specific α6*-nAChR antagonists43, 45, 47. However, in vitro synthesized functional receptors can only shed light on the possibilities of the in vivo receptors because complex subunit combinations of naturally expressed α6*-nAChRs that exist in vivo are not easily recreated in in vitro systems.
In the past decade, several research groups have tried to further define the possible compositions of α6*-nAChRs naturally expressed in neurons. Le Novere and co-workers reported the extensive colocalization of α6 and β3 subunits and were the first to propose the existence of heteromeric α6*-nAChRs containing both α6 and β3 subunits in catecholaminergic nuclei23. Further definition of the exact subunit compositions of naturally-expressed functional α6*-nAChRs remains challenging. Studies on rats and both wild type and several types of nAChR subunit-null mice (eg α4−/−, α6−/−, α4−/−α6−/−, and β2−/−), using combined single-cell RT-PCR, patch-clamp recording, in vivo microdialysis, and immunoprecipitation techniques, have demonstrated that a putative α4α6α5(β2)2 composition is present on the somata of DAergic neurons in the SNc and VTA25. Meanwhile, two types of α6*-nAChRs (α6β2*-nAChRs and α4α6β2*-nAChRs) are expressed in DAergic neuronal terminal fields located in the striatum16. Salminen and colleagues further demonstrated that the presence of presynaptic α-CTX MII-sensitive nAChRs (α6*-nAChRs) on DAergic nerve terminals in striatal synaptosomes plays important roles in mediating DA release. The more interesting finding is that in β2-null mutant mice(β2−/−), the α-CTX MII-sensitive DA release completely disappeared, while only 50% decrease of α-CTX MII-sensitive DA release resulted from β2+/− mutation, which indicates that the β2 subunit is an indispensable component for the α-CTX MII-sensitive nAChR-mediated DA release37. Additional studies found that deletion of β3 or α4 decreased the α-CTX MII-sensitive component of DA release by 76% or 55%, respectively. Neither β4 nor α7 gene deletion significantly altered α-CTX MII-sensitive DA release37. These results suggest that β3 and α4 rather than α7 and β4 subunits play important roles in forming naturally expressed functional α6*-nAChRs (α6β3β2 and α4α6β3β2) on DAergic presynaptic terminals37. The compositions of α6*-nAChRs in chick retina (from 1-day old chicks) are quite different from that in catecholaminergic nuclei. For example, one study showed that only a minor subpopulation of α6*-nAChRs (7.5%) contain the β2 subunit, but almost all of the α6 receptors contain the β4 subunit in chick retina48. The presence of a mixture of different populations of α6*-nAChRs (surely α6β4; probably α6β4β3, α3α6β4, and/or α3α6β3β4) is found in chick retina48. However, Moretti et al reported that α6*-nAChRs expressed in rat retina (postnatal 21d) mainly contain the α6β3β2, α6α4β3β2, and α6α3α2β3β2 subtypes49.
We have described the known diversity of naturally expressed functional α6*-nAChR subunit compositions, which vary in different brain regions or even within the same tissue from different species or different developmental periods within the same specie. This indicates the complex roles of α6*-nAChRs in physiological and perhaps in pathological states. As a result, the improving knowledge of subtype composition of α6*-nAChRs will be of considerable importance for development of selective and specific α6*-nAChR agonists and antagonists.
Analogs of α-conotoxins are subunit-selective antagonists of α6*-nAChRs
It is still a challenge to develop selective agonists and antagonists for α6*-nAChRs due to the complex subunit combinations of naturally expressed α6*-nAChRs and poor function in heterologous expression systems. Until now, the only reported selective agonist for α6*-nAChRs is TC 2429, which is a full agonist with 3-fold more selectivity at α6β2*-nAChRs compared to nicotine39. But α6*-nAChRs can be selectively inhibited by several analogs of α-conotoxins25, 39, 43, 45.Conotoxins can be divided into at least four superfamilies (A, M, O, and S) based on a conserved signal sequence and a characteristic disulfide framework that is distinct from the other superfamiles50. α-conotoxins, which are competitive nAChR antagonists, belong to the largest family of peptides in the A superfamily50. Several α-conotoxins are pharmacologically useful for distinguishing nAChR subtypes. For example, α7*-nAChRs can be inhibited by selective antagonist α-CTX ImI51, α3β4*-nAChRs by antagonist α-CTX AuIB52, and α6*-nAChRs by selective antagonist α-CTX MII and α-CTX PIA39, 45.
Electrophysiological experiments using Xenopus oocytes expressing mammalian neuronal nAChRs have demonstrated that α-CTX MII is a novel, potent, selective, and competitive antagonist for α3β2-nAChRs, which can reversibly block acetylcholine (ACh)-induced inward currents at very low concentration (IC50 is 0.5 nmol/L)53. α-CTX MII is the first α-conotoxin known to target neuronal α3β2-nAChRs53. However, α-CTX MII is now widely used as a selective α6*-nAChR antagonist, especially in midbrain DAergic system because: (1) there is high structural similarity between the α3 and α6 subunits, 61%17, 23 to 80%47 residue identity in their extracellular ligand-binding domains, and critical residues responsible for interaction with α-CTX MII54 are conserved; (2) α6*- but not α3*-nAChRs are highly expressed in midbrain DAergic system: studies demonstrated that α6 labeling is almost 20-fold more intense than α3 labeling in DAergic neurons23, while α3 subunit only accounts for 2% of 3H-Epi binding sites at the DAergic terminal levels16. Moreover, electrophysiological recordings and in vivo microdialysis experiments showed that the inhibitory effect of α-CTX MII on ACh-induced inward currents and nicotine-induced DA release disappear in α6−/− mice16; and (3) α-CTX MII is a selective antagonist for naturally expressed α6*-nAChRs: high-affinity [125I]α-CTX MII binding sites are well preserved in α3-nAChR subunit knockout mice55, but can not be detected in α6-nAChR subunit knockout mice16, 27. Therefore, α-CTX MII, even at a high concentration, eg 100 nmol/L, can be used to study the function of α6*-nAChRs in midbrain DAergic neurons39. Recently, scientists discovered a novel α-conotoxin, α-CTX PIA, which has higher affinity and selectivity for α6* than α3* -nAChRs, exhibiting ∼75-fold lower IC50 for α6/α3β2β3-nAChRs than for α3β2-nAChRs45. α-CTX PIA is able to specifically distinguish α6*-nAChRs from α3*-nAChRs due to its lower affinity for α3*-nAChRs. Another α-conotoxin, α-CTX BuIA, displays strong antagonistic effect on chimeric α6/α3β2β3 and α3β2-nAChRs56, 57 with an IC50 of 0.26 nmol/L and 5.7 nmol/L for α6/α3β2β3-nAChRs and α3β2-nAChRs, respectively57. All of these α-conotoxins have the ability to selectively discriminate α6*-nAChRs from a variety of nAChR subtypes. In addition, the chemical structure of α-conotoxins (only 12–19 amino acids) is relatively simple (with highly conserved nature of the cysteine residues and conserved proteolytic processing sites), which has allowed them to be isolated and their structure sequences identified and synthesized45. Scientists are trying to modify the natural structure and synthesize new analogs of α-conotoxins. For example, substitution of Leu15 with Ala, Glu11 with Ala, and Ala for His9 shifts the selectivity of α-CTX MII toward α6*-nAChRs, with approximately 37-, 54-, and 75-fold higher preference for α6*- than for α3*-nAChRs38, respectively. In addition, Azam et al have successfully designed and synthesized α-CTX MII[S4A, E11A, L15A], which displays more selectivity for α6*-nAChRs with an IC50 of 1.2 nmol/L, and much lower affinity for α3β2-nAChRs with an IC50 of 1400 nmol/L43.
In conclusion, several isolated natural and chemically-synthesized analogs of α-conotoxin have been used as powerful α6 subtype-selective antagonists to investigate physiological and pharmacological properties of the in vitro synthesized- and the in vivo naturally-expressed functional α6*-nAChRs. Such pharmacological tools will, undoubtedly, be of considerable benefit to further understanding of α6*-nAChR function and pharmacology. A more detailed review by Azam et al of the applications of α-conotoxin analogs for nAChR studies can be found in this issue.
The potential role of presynaptic α6*-nAChRs in nicotine reward and dependence
The mesocorticolimbic system including VTA DAergic neurons and their projection areas is postulated to play a crucial role in regulation of cognitive functions, reward-based learning, and addiction58, 59, 60. Numerous nAChRs are expressed in the VTA and some of them are located extra-synaptically on somatodendritic regions and on pre-synaptic terminals. Somatodendritic nAChRs modulate neuronal excitation via membrane depolarization and can initiate short- and long-term changes by interfacing with Ca2+ signaling pathways or the firing pattern of DAergic neurons that determines the release of DA in the terminal regions61, 62. The modulation of neurotransmitter release by pre-synaptic nAChRs is one of the most well-investigated effects of nicotine in the CNS33. Activation of pre-synaptic nAChRs increases the release of many different neurotrans-mitters33, 39, 61, 63, 64. Exogenously-applied nicotinic agonists can enhance, while nicotinic antagonists often can diminish the release of ACh, DA, norepinephrine, serotonin, as well as glutamate and GABA65. The activation of pre-synaptic nAChRs initiates directly or indirectly intracellular Ca2+ signals that potentiate neurotransmitter release through the following mechanisms: (1) a small, direct Ca2+ influx via nAChR activity66, 67, 68 that (2) may trigger Ca2+-induced Ca2+ release from intracellular Ca2+stores69, and (3) the activation of nAChRs further causes membrane depolarization that activates voltage-gated Ca2+channels in pre-synaptic terminals70. The overall effect is that pre-synaptic nAChR activity elevates Ca2+ levels in presynaptic terminals, in turn leading to an increase in neurotransmitter release.
The activation of nAChRs on VTA DAergic neurons by exogenously-applied nicotine results in increased DA release in the nucleus accumbens (NAc), which probably plays a key role in nicotine addiction71, 72. Studies indicate that different subtypes of pre-synaptic nAChRs participate in the modulation of neurotransmitter release. It is supposed that by acting on presynaptic α7-nAChRs (desensitized less than non α7-nAChRs) located on glutamatergic terminals, nicotine at concentrations experienced by smokers can produce long-term enhancement of glutamatergic transmission in the VTA73, whereas activation of presynaptic non-α7 receptors (possibly α4β2-nAChRs) only can transiently enhance GABAergic transmission. These non-α7 nAChRs become significantly and quickly desensitized during long-term exposure to low concentrations of nicotine74. As a result, GABAergic terminals, rather than glutamatergic terminals, become insensitive to tonically released ACh from cholinergic afferents from the pedunculopontine tegmental nucleus (PPTg) and laterodorsal tegmental nucleus (LDTg)75, 76, which will in turn lead to long-term activation of glutamatergic input accompanied with depression of GABAergic input to VTA DAergic neurons that is experienced by tobacco smokers. Collectively, the differential desensitization properties of these two nicotinic receptor subtypes probably explain why low concentrations of nicotine tends to drive the activity of VTA DAergic neurons toward long-term excitation that underlie the course of nicotine addiction process77. Thus, in vivo experiments observed that a single exposure to nicotine increases DA release in NAc from VTA for more than one hour78, 79.
Our current studies demonstrate that there are functional α-CTX MII and PIA sensitive nAChRs (α6*-nAChRs) located on GABAergic pre-synaptic boutons synapsing onto DAergic cell bodies in the VTA. Activation of these α6*-nAChRs by nicotinic agonists results in increased inhibitory postsynaptic currents (IPSCs) measured at the DAergic cell body using patch-clamp recordings. A 4-minute pretreatment with smoking-relevant concentrations of nicotine desensitizes rather than activates α6*-nAChRs and abolishes ACh-induced increases in spontaneuos IPSCs(sIPSCs)80. The results demonstrate that functional α6*-nAChRs are expressed on presynaptic GABAergic boutons in the VTA and likely play a critical role in mediating cholinergic modulation of GABA release. Their desensitization during chronic nicotine exposure may contribute to a disinhibition of VTA DAergic neuronal activity and enhanced DA release. Our findings suggest that α6*-nAChRs play important roles in nicotine-induced reinforcement through the modulation of GABAergic control on VTA DAergic neurons80. The observations are in good agreement with previous reports. An i n vivo study of nicotine-induced increase in locomotor activity in a habituated environment found that 1 week administration of α6 antisense oligonucleotides (directed against the α6 subunit) by osmotic mini-pump suppresses 70% of the nicotine effect, which strongly suggests that enhanced locomotor activity elicited by nicotine is mediated at least in part via α6*-nAChRs17. Studies using striatal synaptosomes demonstrated a preponderant role of α4α6β2*-nAChRs in mediating the α-CTX MII-sensitive part of nicotine-elicited DA release16, but the inhibitory effect of α-CTX MII on nicotine-induced DA release in α6 subunit knockout mice was no longer observed16. α6*-nAChRs in the NAc also play a dominant role in DA release in an action potential frequency-dependent manner81, which is the first direct evidence of the dominant role of α6*-nAChRs in dynamic filtering (frequency-sensitive regulation of DA neuronal activity and terminal DA release) of action potential-dependent DA release in the NAc. In addition, using patch clamp recordings in brain slice preparations from gain-of-function α6*-nAChR mice, Drenan et al demonstrated that in α6 transgenic mice, the α6*-nAChRs expressed on VTA DAergic neurons are ∼10-fold more sensitive to nicotine than in locus coeruleus39, which suggests that functional α6*-nAChRs can be detected on somatodendritic region of DAergic neurons after fuctional enhancement of α6*-nAChR expression. These results suggest that the up-regulation of functional α6*-nAChRs in the mesocorticolimbic system, such as the VTA and NAc, produces a hyperdopaminergic state that may play a critical role in nicotine dependence82. Meanwhile, nicotine self-administration investigated by Pons et al, using α6 and α4 knockout mice, highlighted the crucial roles of both α6*- and α4*-nAChRs in nicotine reinforcement83. It has been suggested that α6*- and α4*-nAChRs can modulate action potential evoked DA-release from either a low action potential threshold or a higher action potential threshold DAergic fiber, respectively84. Thus, nAChRs may exert their roles through 'filtering' action, which will lead to an increase in contrast in DA signals by switching the firing pattern of DAergic neurons from tonic activity to high frequency, reward-related burst activity, thus facilitating the reinforcement properties of nicotine85, 86.
Taken together, these studies suggest that nicotine at concentrations present in the plasma of tobacco smokers preferentially desensitizes both presynaptic α6* and α4β2-nAChRs on GABAergic neurons, whereas, nicotine has minimal desensitization effects on presynaptic α7-nAChRs located on glutamatergic terminals. Thus, endogenously released ACh can facilitate midbrain glutamate, but not GABA release, which leads to an increase in glutamate mediated excitatory inputs onto DAergic neurons in the VTA. As a result, nicotine induces both disinhibition and direct excitation of DAergic neurons, leading to an increased DA release in NAc (Figure 2).
The potential role of presynaptic α6*-nAChRs in Parkinson's disease
Parkinson's disease (PD) was first described as Shaking Palsy in 1817 by the British physician James Parkinson. PD is one of the most common progressive neurodegenerative disorders in the United States, affecting about one million people, with more than 50 000 new diagnosed cases each year87. The pathogenesis of PD typically is slow-paced but relentlessly progressive loss of DAergic neurons in the nigrostriatal DAergic system in the ventral midbrain88. Our understanding of the etiology of PD is still limited. Therefore, interventions to slow, halt or reverse the progression of the disease are crucial. Importantly, epidemiological studies indicate that cigarette smoking offers some protection against developing PD, as smokers with the longest duration of smoking and the highest daily consumption of cigarettes have the lowest PD risk89, which indicates that the protective effects of nicotine are dose- and time-dependent, and the protective effects wane after smoking quit. In addition, results of both prospective and retrospective studies demonstrate that the decreased incidence of PD in smokers does not appear to be due to the increased smoking-related mortality90, 91, 92, 93.
Accumulating lines of evidence indicate that smokers may have a lower incidence of PD through the following possible mechanisms: (1) nicotine directly activates nAChRs expressed on nigrostriatal DAergic neurons, stimulating DAergic neurons to release more DA94, 95, which could partly overcome the nigrostriatal DAergic dysfunction in the disorder; (2) in vitro and in vivo studies suggest that nicotine exposure is neuroprotective against glutamate excitotoxicity, ischemic damage, and DAergic neurotoxic compounds such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 6-hydroxydopamine, paraquat, and methamphetamine90, thus producing a neuroprotective effects96, 97; (3) nicotine may exert its neuroprotective effects on DAergic neurons through anti-inflammatory actions via decreased microglial activation98, which appears to play a possible role in initiating or amplifying DAergic neuronal injury99, 100, 101; (4) nicotine administration can significantly ameliorate PD symptoms such as tremor, rigidity, bradykinesia, and gait disturbance including frozen gait102, 103, 104 and attenuate levodopa-induced dyskinesias90, 105; and (5) nicotine may also act through non-receptor-mediated actions by decreasing ROS generation and oxidative stress and promoting mitochondrial function106, 107, 108. Collectively, evidence suggests that nicotine mainly produces its beneficial effects on PD through nAChRs expressed in nigrostriatal DAergic system. In order to gain further insight into the nAChR subtypes involved in modulating DAergic function and characterization of changes in their expression with nigrostriatal damage, the development of PD therapies that slow or prevent the disorder by using nAChR ligands could be a useful strategy.
Experiments carried out on rodents, primates, and humans have shown that α6*-nAChRs and α4β2*-nAChRs are the major nAChR subtypes in the nigrostriatal system16, 37, 109, 110, 111, 112, 113, 114, while α7-nAChRs are expressed to a lesser extent110, 111, 115. Nicotine may act through α6*-nAChRs mainly expressed on DAergic terminals and stimulate DA release from striatal synaptosomes38. More importantly, α6β2*-nAChRs, but not α4β2*-nAChRs, are significantly decreased and this decrease closely accompanies nigrostriatal DAergic deficits caused by paraquat treatment116, suggesting that chronic nicotine administration may produce its neuroprotective effects on nigrostriatal DAergic neurons through the actication of a select population of α6*-nAChRs in mice116. The same phenomena is seen in both monkeys and humans based on Bordia and co-workers' finding that α6α4β2β3-nAChR is preferentially vulnerable to nigrostriatal damage in monkey treated with MPTP and patients with PD111. These observations indicate that α6*-nAChRs in the nigrostriatal DAergic system are promising targets for selective preventive treatment of PD. The development of selective α6*-nAChR ligands is attracting attention and will hold promise for PD therapies.
Conclusion
Neuronal nAChRs are richly expressed in the central nervous system, however, the α6*-nAChRs are found at the highest concentrations in both mesocorticolimbic and nigrostriatal pathways, and are particularly present in presynaptic nerve terminals. Functional α6*-nAChRs in mesocorticolimbic and nigrostriatal DAergic systems may play crucial roles in nicotine addiction and be of potential therapeutic value in PD. However, we are only beginning to understand the distribution of nAChR α6 subunit in neuronal networks and know very little about its physiological functions and pharmacological properties. At present, the daunting challenge is the development of α6*-nAChRs selective ligands for both basic research and future clinical treatment of human disorders in which α6*-nAChRs have been implicated, such as nicotine reinforcement and PD.
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This project was supported by grants from the Arizona Biomedical Research Commission (0028 and 0057), the Institute for Mental Health Research, and Philip Morris International through their External Research Program (RJL 2005–2008; Jie WU 2007–2010). Authors thank Dr Alain R SIMARD for his help to read and edit the manuscript.
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Yang, Kc., Jin, Gz. & Wu, J. Mysterious α6-containing nAChRs: function, pharmacology, and pathophysiology. Acta Pharmacol Sin 30, 740–751 (2009). https://doi.org/10.1038/aps.2009.63
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DOI: https://doi.org/10.1038/aps.2009.63
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