Abstract
Aim:
To investigate the effect of l-stepholidine (SPD) on the frequency of spontaneous excitatory postsynaptic currents (sEPSC) in the pyramidal cells between layers V and VI in the prelimbic cortex (PL).
Methods:
A whole-cell patch clamp in rat brain slices was used.
Results:
SPD significantly increased the frequency of sEPSC in a concentration-dependent manner. A selective D1 dopamine receptor antagonist SCH23390 blocked SPD-mediated effects, whereas the D1 agonist SKF38393, but not the D2/3 antagonist sulpiride, mimicked SPD-mediated increase in the frequency of sEPSC. Moreover, both protein kinase A (PKA) inhibitor N-(2-[p-bromocinnamylamino]-ethyl)-5-isoquinolinesulfonamide hydrochloride and protein kinase C (PKC) inhibitor chelerythrine attenuated the effect of SPD on sEPSC.
Conclusion:
SPD elicits its effect on the frequency of sEPSC on the PL pyramidal cells via presynaptic D1 receptors, and is dependent on PKA and PKC signaling pathways.
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Project supported by the Ministry of Science and Technology (No 973-2003CB5154000 and the National Natural Science Foundation of China (No 30271495, 30230130).
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Gao, M., Liu, Cl., Yang, S. et al. l-Stepholidine increases the frequency of sEPSC via the activation of D1 dopamine signaling pathway in rat prelimbic cortical neurons. Acta Pharmacol Sin 28, 627–633 (2007). https://doi.org/10.1111/j.1745-7254.2007.00547.x
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DOI: https://doi.org/10.1111/j.1745-7254.2007.00547.x