Abstract
Aim:
Polypeptide from Chlamys farreri (PCF, molecular mass is 879) is a new marine polypeptide compound isolated from Chlamys farreri. This study investigates the possible protective roles and the mechanism of PCF against ultraviolet B (UVB)-induced apoptosis in murine thymocytes.
Methods:
The rate of apoptosis and caspase-3 activation was measured by flow cytometry. The expression of stress-response genes c-fos and c-jun was observed by RT—PCR. Western blot analysis was performed to determine the release of cytochrome c.
Results:
It was found that UVB induced murine thymocyte death. The cells treated with UVB showed an increase in cytochrome c release, caspase-3 activity, as well as in the expression of c-fos and c-jun. In addition, all were involved in UVB-induced cell apoptosis.
Conclusion:
Our present observations pointed to the ability of PCF to avert UVB-induced apoptosis in thymocytes by modulating c-fos and c-jun expression, cytochrome c release, and the consequent activation of caspase-3, which were essential components of the UV-induced cell apoptotic pathway. The results suggested that PCF is a promising protective substance against UV radiation.
Similar content being viewed by others
Article PDF
References
Novak Z, Bonis B, Baltas E, Ocsovszki I . Xenon chloride ultraviolet B laser is more effective in treating psoriasis and in inducing T cell apoptosis than narrow-band ultraviolet B. J Photochem Photobiol B 2002; 67: 32–8.
Snopov SA, de Gruijl FR, Roza L, van der Leun JC . Immunochemical study of DNA modifications in the nuclei of UV-damaged lymphocytes. Photochem Photobiol Sci 2004; 3: 85–90.
Blattner C, Kannouche P, Litfin M . UV-induced stabilization of c-fos and other short-lived mRNAs. Mol Cell Biol 2000; 20: 3616–25.
Wagner EF, Matsuo K . Signalling in osteoclasts and the role of Fos/AP1 proteins. Ann Rheum Dis 2003; 62: 83–5.
Fernandez M, Pirondi S, Antonelli T, Ferraro L, Giardino L, Calza L . Role of c-Fos protein on glutamate toxicity in primary neural hippocampal cells. J Neurosci Res 2005; 82: 115–25.
Bhojani MS, Hamstra DA, Chang DC, Coppola JM, Khan AP, Reddy GR, et al. Imaging of proteolytic activity using a conditional cell surface receptor. Mol Imaging 2006; 5: 129–37.
Lo PK, Huang SZ, Chen HC, Wang FF . The prosurvival activity of p53 protects cells from UV-induced apoptosis by inhibiting c-Jun NH2-terminal kinase activity and mitochondrial death signaling. Cancer Res 2004; 64: 8736–45.
Kutuk O, Poli G, Basaga H . Resveratrol protects against 4-hydroxynonenal-induced apoptosis by blocking JNK and c-JUN/AP-1 signaling. Toxicol Sci 2006; 90: 120–32.
Saraste A, Pulkki K . Morphologic and biochemical hallmarks of apoptosis. Cardiovasc Res 2000; 45: 528–37.
Ding BX, Wang CB . Inhibitory effect of polypeptide from Chlamys farreri on UVB-induced apoptosis and DNA damage in normal human dermal fibroblasts in vitro. Acta Pharmacol Sin 2003; 24: 1006–10.
Wang CB, Ding BX, Guo SB, Wang YZ, Han YT, Wang YJ . Protective effect of polypeptide from Chlamys farreri on mitochondria in human dermal fibroblasts by ultraviolet B. Acta Pharmacol Sin 2003; 24: 692–96.
Chen HY, Zhu L, Zhan SM, Han ZW, Du W, Wang YJ, et al. Polypeptide from Chlamys farreri inhibits murine thymocytes apoptosis and modulates UVB induced signaling pathway activation. Life Sci 2005; 77: 768–79.
Huang H, Petkova SB, Cohen AW, Bouzahzah B, Chan J, Zhou JN, et al. Activation of transcription factors AP-1 and NF-kappa B in murine Chagasic myocarditis. Infect Immun 2003; 71: 2859–67.
Wu B, Ootani A, Iwakiri R, Fujise T, Tsunada S, Toda S, et al. Ischemic preconditioning attenuates ischemia-reperfusion-induced mucosal apoptosis by inhibiting the mitochondria-dependent pathway in rat small intestine. Am J Physiol Gastrointest Liver Physiol 2004; 286: G580–7.
Carracedo J, Ramirez R, Soriano S, Martin-Malo A, Rodriguez M, Aljama P . Caspase-3-dependent pathway mediates apoptosis of human mononuclear cells induced by cellulosic haemodialysis membranes. Nephrol Dial Transplant 2002; 17: 1971–7.
Silvers AL, Bachelor MA, Bowden GT . The role of JNK and p38 MAPK activities in UVA-induced signaling pathways leading to AP-1 activation and c-fos expression. Neoplasia 2003; 5: 319–29.
Li T, Dai W, Lu L . Ultraviolet-induced jun D activation and apoptosis in myeloblastic leukemia ML-1 cells. J Biol Chem 2002; 277: 32 668–76.
Kawasaki H, Komai K, Ouyang Z, Murata M, Hikasa M, Ohgiri M, et al. c-Fos/activator protein-1 transactivates weel kinase at G1/S to inhibit premature mitosis in antigen-specific Th1 cells. EMBO J 2001; 20: 4618–27.
Rieger KE, Chu G . Portrait of transcriptional responses to ultraviolet and ionizing radiation in human cells. Nucleic Acids Res 2004; 32: 4786–803.
Li Y, Arita Y, Koo HC, Davis JM, Kazzaz JA . Inhibition of c-Jun N-terminal kinase pathway improves cell viability in response to oxidant injury. Am J Respir Cell Mol Biol 2003; 29: 779–83.
Huang Y, Keen JC, Hager E, Smith R, Hacker A, Frydman B, et al. Regulation of polyamine analogue cytotoxicity by c-Jun in human MDA-MB-435. Mol Cancer Res 2004; 2: 81–8.
Sethi G, Sodhi A . Activation of c-Jun N-terminal kinase is required for ultraviolet B-induced apoptosis of murine peritoneal macrophages in vitro. J Photochem Photobiol B 2004; 73: 133–40.
Author information
Authors and Affiliations
Corresponding author
Additional information
This work is supported by the National Natural Science Foundation of China (No 30471458).
Rights and permissions
About this article
Cite this article
Chen, Hy., Chu, X., Yan, Cl. et al. Polypeptide from Chlamys farreri attenuates murine thymocytes damage induced by ultraviolet B. Acta Pharmacol Sin 28, 1665–1670 (2007). https://doi.org/10.1111/j.1745-7254.2007.00621.x
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1111/j.1745-7254.2007.00621.x