Abstract
Aim:
To investigate the effect of the Tob1 gene, a member of the Transducing Molecule of ErbB2/B-cell Translocation Ggene (TOB/BTG) family, by using the adenovirus-mediated expression of Tob1 on radiosensitivity in a human breast cancer cell line MDA-MB-231.
Methods:
Cell survival was determined by clonogenic assay. Apoptosis was evaluated by DNA fragmentation gel electrophoresis and terminal deoxynucleotidyl transferase-mediated nick end labeling assay. Protein expression was analyzed by Western blot assay and DNA repair was measured by a host cell reactivation assay.
Results:
We demonstrated that pre-irradiation treatment with Ad5–Tob1 significantly increased radiosensitivity, accompanying the increased induction of apoptosis and the repression of DNA damage repair. Furthermore, Ad5-Tob1-mediated radiosensitivity correlates with the upregulation of the pro-apoptotic protein Bax and the downregulation of several DNA double strand break repair proteins, including DNA-dependent protein kinases, Ku70 and Ku80, and X-ray-sensitive complementation group 4.
Conclusion:
Tob1, as a new radiosensitizer, is a new target in the radiotherapy of breast cancer via increasing apoptosis and suppressing DNA repair.
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Jiao, Y., Ge, Cm., Meng, Qh. et al. Adenovirus-mediated expression of Tob1 sensitizes breast cancer cells to ionizing radiation1. Acta Pharmacol Sin 28, 1628–1636 (2007). https://doi.org/10.1111/j.1745-7254.2007.00647.x
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DOI: https://doi.org/10.1111/j.1745-7254.2007.00647.x
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