Abstract
Aim:
To investigate the neuroprotective effect of propofol and its intracellular mechanism on neurons in vitro.
Methods:
Cell viability was determined with 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide reduction. Apoptotic cell death was determined by Hoechst 33258 staining and a fluorescence-activated cell sorter. The caspase-3 activity was measured by fluorometric assay. Mitogen-activated protein (MAP) kinase phosphorylation was detected with Western blotting.
Results:
The pretreatment of rat pheochromocytoma cell line PC12 with propofol (1-10 μmol/L) resulted in a significant recovery from hydrogen peroxide (H2O2)-induced cell death and the inhibition of H2O2 induced caspase-3 activation and PC12 cell apoptosis. Propofol inhibited the H2O2-induced p38 MAP kinase, but not c-Jun N-terminal kinase or extracellular signal-regulated kinase 1 and 2 activations.
Conclusion:
Propofol might attenuate H2O2-induced PC 12 cell death through the inhibition of signaling pathways mediated by the p38 MAP kinase.
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This work was supported by a grant from the National Natural Science Foundation of China (No 30371731).
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Wu, Xj., Zheng, Yj., Cui, Yy. et al. Propofol attenuates oxidative stress-induced PC12 cell injury via p38 MAP kinase dependent pathway. Acta Pharmacol Sin 28, 1123–1128 (2007). https://doi.org/10.1111/j.1745-7254.2007.00610.x
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DOI: https://doi.org/10.1111/j.1745-7254.2007.00610.x
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