Abstract
Aim:
To examine the inhibitive effects of triptolide on the expression of IL-8, monocyte chemotactic protein (MCP)-1, and matrix metalloproteinases (MMP)-3 in subepithelial myofibroblasts (SEMF) stimulated with IL-1β.
Methods:
SEMF cultures were established from normal colons in patients who underwent gut resection for colorectal carcinoma. Chemokine and MMP-3 expressions were determined by ELISA and RT-PCR. The cytosolic amount of phosphorylation of IκB-α (p-IκB-α) was determined by Western blotting. The DNA binding capacity of NF-κB was evaluated by electrophoretic mobility shift assays.
Results:
IL-1β stimulated protein and mRNA expression of IL-8, MCP-1, and MMP-3 in SEMF. Triptolide inhibited these effects of IL-1β in a dose-dependent manner. Mechanistic studies revealed that triptolide markedly decreased IL-1β-induced NF-κB DNA binding capacity and cytosolic amount of p-IκB-α. These results showed that triptolide inhibited IL-1 β-induced chemokine and MMP-3 expression in SEMF through the NF-κB pathway.
Conclusion:
Triptolide inhibited IL-1 β-induced chemokine and MMP-3 expression in SEMF by preventing the phosphorylation of IκB-α.
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Project supported by the National Natural Science Foundation of China (No 30571797).
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Tao, Qs., Ren, Ja. & Li, Js. Triptolide suppresses IL-1β-induced chemokine and stromelysin-1 gene expression in human colonic subepithelial myofibroblasts. Acta Pharmacol Sin 28, 81–88 (2007). https://doi.org/10.1111/j.1745-7254.2007.00482.x
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DOI: https://doi.org/10.1111/j.1745-7254.2007.00482.x