Abstract
Aim:
To identify a shuttle promoter that can mediate gene expression in both insect cells and mammalian cells to facilitate the development of a baculovirus vector-based mammalian cell gene delivery vehicle.
Methods:
Recombinant baculoviruses carrying the β-galactosidase reporter gene under the control of an early to late (ETL) promoter of the Autographa californica multiple nuclear poly-hedrosis virus (AcMNPV) or a cytomegalovirus immediate early promoter (CMV promoter) were constructed. COS1, HeLa, CHO-K1, hFob1.19, andMCF-7 mammalian cells were tested for the expression of β-galactosidase.
Results:
ETL promoter activity was higher in bone-derived hFob1. 19 than in COS1, HeLa, CHO-K1, or MCF-7 mammalian cells. The transient plasmid transfection assay indicated that ETL promoter activity in mammalian cells was dependent on baculovirus gene expression.
Conclusion:
ETL promoter activity in mammalian cells is baculovirus gene expression-dependent, and the shuttle promoter will facilitate the application of baculovirus expression vectors in mammalian cell expression systems and for gene therapy.
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Project supported by grants NSC 93-2745-M-033-001 and NSC-93-2745-M-033-004 to Tzong-yuan Wu and 93-2311-B-182-004 to Yu-kou Liu from the National Science Council, Taiwan, China.
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Liu, Yk., Chu, Cc. & Wu, Ty. Baculovirus ETL promoter acts as a shuttle promoter between insect cells and mammalian cells. Acta Pharmacol Sin 27, 321–327 (2006). https://doi.org/10.1111/j.1745-7254.2006.00276.x
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DOI: https://doi.org/10.1111/j.1745-7254.2006.00276.x