Abstract
Aim:
To investigate and compare the antioxidant activities of salvianolic acid B (SalB) and Ginkgo biloba extract (EGb 761) in aqueous solution, rat microsomes and the cellular system.
Methods:
Superoxide anion () was generated using xanthine/xanthine oxidase system and phenazine methosulate/NADH system, and the effects of SalB and EGb 761 on the generation of were achieved by spectrophotometric measurement of the product formed on reduction of nitro blue tetrazolium. Two different methods were used to assess the scavenging effects of the extracts on hydroxyl radical (·OH): HPLC method was used for quantitation of ·OH by oxy-radical trapping of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) to form DMPO-OH adducts in Fe2+-EDTA-H2O2 system. To confirm the HPLC data, ·OH was also measured by spectrophotometry using a commercial detection kit. The anti-lipid peroxidation effects of the extracts in microsomes of rat brain, liver and kidney induced by ascorbate-NADPH were determined by thiobarbituric acid (TBA) method. The protective effects of the extracts on peroxide hydrogen (H2O2)-induced oxidative damage in SH-SY5Y cells were investigated by assessing cell viability assay, the level of lipid peroxidation, and the lactate dehydrogenase (LDH) release.
Results:
Both SalB and EGb 761 were able to scavenge and ·OH, inhibit lipid peroxidation of microsomes, and protect SH-SY5Y cells against H2O2-induced oxidative damage. However, the concentration of SalB was far lower than that of EGb 761 when a similar effect was obtained.
Conclusion:
The antioxidant efficiency of SalB was greater than that of EGb 761. These results suggest that SalB, like EGb 761, has promising potential in treating oxidative damage-derived neurodegenerative disorders.
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References
Stohs SJ . The role of free radicals in toxicity and disease. J Basic Clin Physiol Pharmacol 1995; 6: 205–28.
Finkel T, Holbrook NJ . Oxidants, oxidative stress and the biology of ageing. Nature 2000; 408: 239–47.
Kim K, Rhee SG, Stadtman ER . Nonenzymatic cleavage of proteins by reactive oxygen species generated by dithiothreitol and iron. J Biol Chem 1985; 260: 15394–7.
Imlay JA, Linn S . DNA damage and oxygen radical toxicity. Science 1988; 240: 1302–9.
Block G . The data support a role for antioxidants in reducing cancer risk. Nutr Rev 1992; 50: 207–13.
Li J, He LY, Song WZ . Separation and quantitative determination of seven aqueous depsides in Salvia miltiorrhiza by HPTLC scanning. Acta Pharm Sin 1993; 28: 543–7. Chinese.
Li LN . Water soluble active components of Salvia miltiorrhiza and related plants. J Chin Pharm Sci 1997; 6: 57–64. Chinese.
Du GH, Qiu Y, Zhang JT . Salvianolic acid B protects the memory functions against transient cerebral ischemia in mice. J Asian Nat Prod Res 2000; 2: 145–52.
Chen YH, Du GH, Zhang JT . Salvianolic acid B protects brain against injuries caused by ischemia-reperfusion in rats. Acta Pharmacol Sin 2000; 21: 463–6.
Tang MK, Zhang JT . Salvianolic acid B inhibits fibril formation and neurotoxicity of amyloid beta-protein in vitro. Acta Pharmacol Sin 2001; 22: 380–4.
Kleijnen J, Knipschild P . Ginkgo biloba. Lancet 1992; 340: 1136–9.
Smith JV, Luo Y . Studies on molecular mechanisms of Ginkgo biloba extract. Appl Microbiol Biotechnol 2004; 64: 465–72.
Pincemail J, Dupuis M, Nasr C, Hans P, Haag-Berrurier M, Anton R, et al. Superoxide anion scavenging effect and superoxide dismutase activity of Ginkgo biloba extract. Experientia 1989; 45: 708–12.
Marcocci L, Packer L, Droy-Lefaix MT, Sekaki A, Gardes-Albert M . Antioxidant action of Ginkgo biloba extract EGb 761. Methods Enzymol 1994; 234: 462–75.
Zang LY, Stone K, Pryor WA . Detection of free radicals in aqueous extracts of cigarette tar by electron spin resonance. Free Radic Biol Med 1995; 19: 161–7.
Zang LY, Cosma G, Gardner H, Vallyathan V . Scavenging of reactive oxygen species by melatonin. Biochim Biophys Acta 1998; 1425: 469–77.
Nishikimi M, Appaji N, Yagi K . The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 1972; 46: 849–54.
Liu Y, Zhang JT . Direct measurement of oxy-radicals using HPLC-ECD. Acta Pharm Sin 1993; 28: 416–21. Chinese.
Gnojkowski J, Baer-Dubowska W, Klimek D, Chmiel J . Effect of toluidines on drug metabolizing enzymes in rat liver, kidney and lung. Toxicology 1984; 32: 335–42.
Huang YS, Zhang JT . Antioxidative effect of three water-soluble components isolated from Salvia miltiorrhiza in vitro. Acta Pharm Sin 1992; 27: 96–100. Chinese.
Ohkawa H, Ohishi N, Yagi K . Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351–8.
Mattson MP, Barger SW, Begley JG, Mark RJ . Calcium, free radicals, and excitotoxic neuronal death in primary cell culture. Methods Cell Biol 1995; 46: 187–216.
McCord JM, Day, ED Jr . Superoxide-dependent production of hydroxyl radical catalyzed by iron-EDTA complex. FEBS Lett 1978; 86: 139–42.
Wills ED . Lipid peroxide formation in microsomes. General considerations. Biochem J 1969; 113: 315–24.
Barth SA, Inselmann G, Engemann R, Heidemann HT . Influences of Ginkgo biloba on cyclosporin A induced lipid peroxidation in human liver microsomes in comparison to vitamin E, glutathione and N-acetylcysteine. Biochem Pharmacol 1991; 41: 1521–6.
Satoh T, Sakai N, Enokido Y, Uchiyama Y, Hatanaka H . Free radical-independent protection by nerve growth factor and Bcl-2 of PC12 cells from hydrogen peroxide-triggered apoptosis. J Biochem 1996; 120: 540–6.
Behl C, Davis JB, Lesley R, Schubert D . Hydrogen peroxide mediates amyloid beta protein toxicity. Cell 1994; 77: 817–27.
Bagchi D, Bagchi M, Hassoun EA, Stohs SJ . In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicology 1995; 104: 129–40.
Wei T, Ni Y, Hou J, Chen C, Zhao B, Xin W . Hydrogen peroxide-induced oxidative damage and apoptosis in cerebellar granule cells: protection by Ginkgo biloba extract. Pharmacol Res 2000; 41: 427–33.
Seif-El-Nasr M, El-Fattah AA . Lipid peroxide, phospholipids, glutathione levels and superoxide dismutase activity in rat brain after ischaemia: effect of Ginkgo biloba extract. Pharmacol Res 1995; 32: 273–8.
Kose K, Dogan P . Lipoperoxidation induced by hydrogen peroxide in human erythrocyte membranes. 2. Comparison of the antioxidant effect of Ginkgo biloba extract (EGb 761) with those of water-soluble and lipid-soluble antioxidants. J Int Med Res 1995; 23: 9–18.
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Project supported by a grant from the National Key Program of Basic Research and Development in China (No G199805109).
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Liu, Cs., Cheng, Y., Hu, Jf. et al. Comparison of antioxidant activities between salvianolic acid B and Ginkgo biloba extract (EGb 761). Acta Pharmacol Sin 27, 1137–1145 (2006). https://doi.org/10.1111/j.1745-7254.2006.00378.x
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DOI: https://doi.org/10.1111/j.1745-7254.2006.00378.x
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