Abstract
Aim:
To study the anti-endotoxic effect of o-aminobenzoic acid (OABA) isolated from Banlangen(BLG).
Methods:
OABA was extracted and isolated from BLG and diluted into 0.5% solution. The concentration of endotoxin (ET) pretreated with OABA was quantitatively detected using Limulus test. The inhibition of ET-induced fever by OABA was measured in rabbits. The rates of lipopolysaccharides (LPS)-induced death in mice pretreated with or without OABA were then compared. The influence of OABA on the release of TNF-α and NO from macrophages induced by LPS was examined in mice.
Results:
After pretreatment with OABA, 84.4% of ET was destroyed. The ET-induced fever in rabbits decreased significantly and the rate of LPS-induced death in mice dropped from 70% to 20%. The release of TNF-α and NO induced by LPS in mice was inhibited dose-dependently when the concentration of OABA was between 0.125% and 0.5%.
Conclusion:
OABA isolated from BLG has an anti-endotoxic effect.
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Project was supported by the National Natural Science Foundation of China (No 39170877, 39800193, and 39870872) and the Foundation of Ministry of Health People's Republic of China (No 98-2-110).
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Fang, Jg., Liu, Yh., Wang, Wq. et al. The anti-endotoxic effect of o-aminobenzoic acid from Radix Isatidis. Acta Pharmacol Sin 26, 593–597 (2005). https://doi.org/10.1111/j.1745-7254.2005.00080.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00080.x