Abstract
Aim:
To investigate effects of taurine on ischemia/reperfusion (I/R)-induced compartment syndrome in rabbit hind limbs.
Methods:
Rabbits underwent femoral artery occlusion after ligation of branches from terminal aorta to femoral artery. After a 7-h ischemia, reperfusion was established with the use of heparinized polyethylene shunts. Rabbits received taurine (1 g/kg) or normal saline (control) by iv infusion 10 min before shunt placement. During reperfusion, anterior compartment pressure (ACP) was monitored continuously in the left lower extremity. Gastrocnemius muscle triphenyltetrazolium chloride (TTC) level, taurine content and myeloperoxidase activity were assayed. Oxidative stress was induced in the in vitro gastrocnemius muscle slices by free radical generating systems (FRGS), and the malondialdehyde content was measured in presence or absence of taurine.
Results:
After 7 h of ischemia, none of the parameters that we measured were different from those before ischemia, except that TTC reduction decreased by 80%. In the control group, after 2 h of reperfusion, ACP increased 4.5-fold, and gastrocnemius muscle taurine content was reduced by 33%. In taurine-treated animals, at 2 h reperfusion, the mean arterial blood pressure and heart rate were increased, by 6% and 10%. ACP decreased by 39%, muscle edema decreased by 16%, TTC reduction increased by 150%, and lactate dehydrogenase decreased by 36% compared to control group. Plasma and muscle taurine content increased by 70% and 88%, respectively. In the taurine-treated group, at 2 h reperfusion, plasma malondialdehyde and conjugated diene content were decreased by 38% and 23%, respectively, and muscle malondialdehyde and conjugated diene content decreased by 22% and 30%, respectively compared to the control group. At 2 h reperfusion, myeloperoxidase activity was increased 3.5-fold in control animals. In the in vitro study, taurine decreased malondialdehyde content in muscle slices incubated with hypochlorous acid in a dose-dependent manner, but there was no change when incubated with hydrogen peroxide and xanthine oxidase.
Conclusion:
Treatment with taurine inhibited I/R-induced compartment syndrome by at least in part attenuating oxidative stress injury induced by I/R, suggesting clinical application of taurine might be a new strategy for the prevention and treatment of compartment syndrome.
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References
Mubarek SJ, Hargens AR . Acute compartment syndromes. Surg Clin North Am 1983; 63: 539–65.
Timoshin AA, Lakomkin VL, Ruuge EK . Effect of a series of brief cycles of ischemia and reperfusion on free radical centers of isolated rat myocardial tissue. Biofizika 1998; 43: 134–8.
Van Haaften RI, Haenen GR, Evelo CT, Bast A . Effect of vitamin E on glutathione-dependent enzymes. Drug Metab Rev 2003; 35: 215–53.
Dogru Abbasoglu S, Kanbagli O, Balkan J, Cevikbas U, Aykac-Toker G, Uysal M . The protective effect of taurine against thioacetamide hepatotoxicity of rats. Hum Exp Toxicol 2001; 20: 23–7.
Oz E, Erbas D, Gelir E, Aricioglu A . Taurine and calcium interaction in protection of myocardium exposed to ischemic reperfusion injury. Gen-Pharmacol 1999; 33: 137–41.
Wettstein M, Haussinger D . Taurine attenuates cold ischemiareoxygenation injury in rat liver. Transplantation 2000; 69: 2290–6.
Michalk DV, Hoffmann B, Minor T . Taurine reduces renal ischemia/reperfusion injury in the rat. Adv Exp Med Biol 2003; 526: 49–56.
Zhang LY, Dong SY, Xu J, Yu F, Pang YZ, Tang CS, et al. Effect of taurine on rats with tourniquet shock. China J Pathphysiol 1993; 9: 208.
Hakaim AG, Corsetti, R, Cho S . The pentafraction of hydroxyethyl starch inhibits ischemia-induced compartement syndrome. J Trauma 1994; 37: 18–21.
Rorabeck CH, Castle GSP, Hardie R, Logan J . Compartment pressure measurements: an experimental investigation using the slit catheter. J Trauma 1981; 21: 446–9.
Belkin M, Brown RD, Wright JC . A new quantitative spectrophotometric assay for ischemia-reperfusion injury in skeletal muscle. Am J Surg 1988; 156: 83–8.
Naito Y, Yoshikawa T, Matsuyama K, Yagi N, Arai M, Nakamura Y, et al. Neutrophils, lipid peroxidation, and nitric oxide in gastric reperfusion injury in rats. Free Radic Biol Med 1998; 24: 494–502.
Skawa T, Matashushita S . Thiobarbituric acid test for detecting lipid peroxide. Lipids 1979; 14: 401–6.
Waller RL, Recknagel RO . Determination of lipid conjugated dienes with tetracyanoethylene-14C: significance for study of the pathology of lipid peroxidation. Lipids 1977; 12: 914–21.
Chang L, Xu JX, Zhao J, Pang YZ, Tang CS, Qi YF . Taurine antagonized oxidative stress injury induced by homocysteine in rat vascular smooth muscle cells. Acta Pharmacol Sin 2004; 25: 341–6.
Perler BA, Tohmeh AG, Buckley GB . Inhibition of the compartment syndrome by ablation of free radical mediated reperfusion injury. Surgery 1990; 108: 40–7.
Huxtable RJ . Physiological actions of taurine. Physiol Rev 1992; 72: 101–63.
Bitoum M, Levillain O, Tappaz M . Gene expression of the taurine transporter and taurine biosynthetic enzymes in rat kidney after antidiuresis and salt loading. Pflugers Arch Eur J Physiol 2001; 442: 87–95.
Lourenco R, Camilo ME . Taurine: a conditionally essential amino acid in humans? An overview in health and disease. Nutr Hosp 2002; 17: 262–70.
Aneja R, Hake PW, Burroughs TJ, Denenberg AG, Wong H, Zingarelli B . Epig allocatechin, a green tea polyphenol, attenuates myocardial ischemia reperfusion injury in rats. Mol Med 2004; 10: 55–62.
Winterbourn CC, Vissers MC, Kettle AJ . Myeloperoxidase. Curr Opin Hematol 2000; 7: 53–8.
Li JX, Pang YZ, Tang CS, Li ZQ . Protective effect of taurine on hypochlorous acid toxicity to nuclear nucleoside triphosphatase in isolated nuclei from rat liver. World J Gastroenterol 2004; 10: 694–8.
Chang L, Xu J, Yu F, Zhao J, Tang X, Tang CS . Taurine protected myocardial mitochondria injury induced by hyperhomocysteinemia in rats. Amino Acids 2004; 27: 37–48.
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Project supported by the State Major Basic Research Program of China (No G 200056905).
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Wang, Jx., Yan, L., Zhang, Lk. et al. Taurine inhibits ischemia/reperfusion-induced compartment syndrome in rabbits. Acta Pharmacol Sin 26, 821–827 (2005). https://doi.org/10.1111/j.1745-7254.2005.00128.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00128.x
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