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Construction and production of oncotropic vectors, derived from MVM(p), that share reduced sequence homology with helper plasmids

Cancer Gene Therapy volume 9, pages 762–770 (2002)Cite this article

Abstract

The production of currently available vectors derived from autonomous parvoviruses requires the expression of capsid proteins in trans, from helper sequences. Cotransfection of a helper plasmid always generates significant amounts of replication-competent virus (RCV) that can be reduced by the integration of helper sequences into a packaging cell line. Although stocks of minute virus of mice (MVM)-based vectors with no detectable RCV could be produced by transfection into packaging cells; the latter appear after one or two rounds of replication, precluding further amplification of the vector stock. Indeed, once RCVs become detectable, they are efficiently amplified and rapidly take over the culture. Theoretically RCV-free vector stocks could be produced if all homology between vector and helper DNA is eliminated, thus preventing homologous recombination. We constructed new vectors based on the structure of spontaneously occurring defective particles of MVM. Based on published observations related to the size of vectors and the sequence of the viral origin of replication, these vectors were modified by the insertion of foreign DNA sequences downstream of the transgene and by the introduction of a consensus NS-1 nick site near the origin of replication to optimize their production. In one of the vectors the inserted fragment of mouse genomic DNA had a synergistic effect with the modified origin of replication in increasing vector production.

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Acknowledgements

N Clément was supported by a grant from the FNRS/Télévie. This work was subsidized by a grant from the “Fédération contre le Cancer” from the Grand Duchy of Luxembourg. We also benefited from support by “FEDER” in the framework of the program “Hainaut-Nord-Pas de Calais-Picardie” and the Association Française contre les Myopathies (AFM). AB would like to thank the Fondation Lions “Vaincre le Cancer” (Grand Duchy of Luxembourg) for their support.

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  1. IRIBHN-IBMM, Université Libre de Bruxelles, rue des professeurs Jeener et Brachet, 12, Gosselies, Belgium

    Nathalie Clément, Thierry Velu & Annick Brandenburger

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  1. Nathalie Clément
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  2. Thierry Velu
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  3. Annick Brandenburger
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Correspondence to Annick Brandenburger.

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Clément, N., Velu, T. & Brandenburger, A. Construction and production of oncotropic vectors, derived from MVM(p), that share reduced sequence homology with helper plasmids. Cancer Gene Ther 9, 762–770 (2002). https://doi.org/10.1038/sj.cgt.7700496

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