Abstract
Esthesioneuroblastoma (ENB) is a rare, site-specific, locally aggressive neuronal malignancy so far thought to belong to primitive peripheral neuroectodermal tumour-Ewing’s tumour (pPNETs-ETs). Its anatomical location, in addition to morphologic, immunophenotypic and ultrastructural features, suggests its origin in the neuronal or neuroendocrine cells of the olfactory epithelium. However, the cytogenetic and molecular data currently available appear controversial on the presence of the typical translocation t(11;22)(q24;q12) and of trisomy 8, chromosomal changes that characterize the tumours belonging to the pPNETs-ETs. Herein we have analysed five ENB tumour specimens for trisomy 8 by fluorescence in situ hybridization (FISH), for the presence of EWS gene rearrangements by FISH, reverse transcription polymerase chain reaction and Southern blot analyses, as well as for the expression of the Ewing sarcoma-associated MIC2 antigen by immunohistochemistry. Neither EWS/FLI-I, EWS/ERG and EWS/FEV fusion genes nor MIC2 expression were found in any tumour, whereas trisomy 8 was found in one case only. Moreover, DNA from three cases analysed by Southern blot did not show EWS gene rearrangements. Our results support the evidence that ENB is not a member of the pPNETs-ETs.
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References
Adams, V, Kempf, W, Hassam, S & Briner, J (1995) Determination of hexokinase isoenzyme I and II composition by RT-PCR: increased hexokinase isoenzyme II in human renal cell carcinoma. Biochem Mol Med 54: 53–58.
Argani, P, Perez-Ordonez, B, Xiao, H, Caruana, SM, Huvos, AG & Ladanyi, M (1998) Olfactory neuroblastoma is not related to the Ewing family of tumors absence at EWS/FLI1 gene fusion and MIC2 expression. Am J Surg Pathol 22: 391–398.
Banerjee, AK, Sharma, BS, Vashista, RK & Kak, VK (1992) Intracranial olfactory neuroblastoma: evidence for olfactory epithelial origin. J Clin Pathol 45: 299–302.
Burger, PC & Scheithauer, BW (1993). Atlas of Tumor Pathology. Tumors of the Central Nervous System, Armed Forces Institute of Pathology: Washington 200–202.
Cattoretti, G, Beker, MHG, Key, G, Duchrow, M, Schluter, C, Galle, J & Gerdes, J (1992) Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave processed formalin-fixed praffin sections. J Pathol 168: 357–363.
Cavazzana, AO, Navarro, S, Noguera, R, Reynolds, PC & Triche, TJ (1988) Olfactory neuroblastoma is not a neuroblastoma but is related to primitive neuroectodermal tumor (PNET). Prog Clin Biol Res 271: 463–473.
Chan, JCK, Tsang, WJW, Sereviratne, S & Pau, MY (1995) The MIC2 antibody 013 practical application for the study of thymic epithelial tumors. Am J Surg Pathol 19: 1115–1123.
Dal Cin, P, Sciot, R, Aly, MS, Delabie, J, Stas, M, De Wever, I, Van Damne, B & Van Den Berghe, H (1994) Some desmoid tumors are characterized by trisomy 8. Genes Chromasomes Cancer 10: 131–135.
Delattre, O, Zucman, J, Plougastel, B, Desmaze, C, Melot, T, Peter, M, Kovar, H, Joubert, I, De Jong, P, Rouleau, G, Aurias, A & Thomas, G (1992) Gene fusion with and ETS DNA-binding domain caused by chromosome translocation in human tumors. Nature 359: 162–165.
Delattre, O, Zucman, J, Melot, T, Sastre Garau, X, Zucker, JM, Lenoir, GM, Ambros, PF, Sheer, D, Turc-Carel, C, Triche, TJ, Aurias, A & Thomas, G (1994) The Ewing family of tumors. A subgroup of small-round cell tumors defined by chimeric transcript. N Eng J Med 331: 294–299.
Desmaze, C, Zucman, J, Delattre, O, Melot, T, Thomas, G & Aurias, A (1994) Interphase molecular cytogenetics of Ewing’s sarcoma and peripheral neuroepithelioma t (11;22) with flanking and overlapping cosmid probes. Cancer Genet Cytogenet 74: 13–18.
Frierson, HF, Ross, GW, Mills, SE & Frankfurter, A (1990) Olfactory neuroblastoma. Additional immunohistochemical characterization. Am J Clin Pathol 94: 547–553.
Hasle, H, Clausen, N, Pedersen, B & Bendix-Hansen, K (1995) Myelodysplastic syndrome in a child with constitutional trisomy 8 mosaicism and normal phenotype. Cancer Genet Cytogenet 79: 79–81.
Heim, S & Mitelman, F (1995). Cancer cytogenetics chromomomal and molecular genetic aberrations of tumor cells, New York: Alan R. Liss, Inc.pp. 494–495.
Hyams, VJ, Batsakis, JG & Michaels, LE (1998). Atlas of Tumor Pathology. Tumors of the Upper Respiratory Tract, 2nd series, fascicle 25Washington, DC, Armed Forces Institute of Pathology 239–248.
Jeon, IS, Davis, JN, Braun, BS, Sublett, JE, Roussel, MF, Denny, CT & Shapiro, DN (1995) A variant Ewing’s sarcoma translocation (7;22) fuses the EWS gene to the ETS gene ETV1. Oncogene 10: 1229–1234.
Jin, Y, Mertens, F, Arheden, K, Mandhal, N, Wennerberg, J, Dictor, M, Heim, S & Mitelman, F (1995) Karyotypic features of malignant tumors of the nasal cavity and paranasal sinuses. Int J Cancer 60: 637–641.
Kadish, S, Goodman, M & Wangt, CC (1976) Olfactory neuroblastoma. Cancer 37: 1571–???.
Kaneko, Y, Yoshida, K & Handa, M et al (1996) Fusion of an ETS-family gene, EIAF, to EWS by t(17;22) (q12:q12) chromosome translocation in an undifferentiated sarcoma of infancy. Genes Chrom Cancer 15: 115–121.
Lane, S & Ironside, JW (1990) Extra-skeletal Ewing’s sarcoma of the nasal fossa. Laryngol Otol 104: 570–573.
Lichter, P, Change Tang, C, Call, K, Hermanson, G, Evans, GA, Housman, D & Ward, DC (1990) high-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247: 64–67.
Mark, HFL (1996) Constitutional trisomy 8 mosaicism and cancer. Cancer Genet Cytogenet 86: 87–88.
Mezzelani, A, Tornielli, S, Sard, L, Radice, MT, Minoletti, F, Pierotti, MA & Pilotti, S (1997) Is esthesioneuroblastoma a member of the primitive peripheral neuroectodermal tumors? Cytogenet Cell Genet 77: 145
Mitelman, F (1985). Human Chromosome Abnormalities: Catalogues and Collections, pp. 203–248. Alan R. Liss, Inc, New York
Nelson, RS, Perlman, EJ & Askin, FB (1995) Is esthesioneuroblastoma a peripheral neuroectodermal tumor? Hum Pathol 26: 639–641.
Peter, M, Couturier, J, Pacquement, H, Michon, J, Thomas, G, Magdelenat, H & Delattre, O (1997) A new member of the ETS family fused to EWS in Ewing tumors. Oncogene 14: 1159–1164.
Pontius, K & Sebek, B (1982) Extraskeletal Ewing’s sarcoma of the nasal fossa. Am J Clin Pathol 75: 410–415.
Rao, VN, Papas, TS & Reddy, ES (1987) Erg, a human ets-related gene on chromosome 21: alternative splicing, polyadenylation, and translation. Science 237: 635–639.
Ried, T, Baldini, A, Rand, TC & Ward, DC (1992) Simultaneous visualization of seven different probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy. Proc Natl Acad Sci USA 89: 1388–1392.
Shanmugaratnam, K (1991) International Histological Classification of Tumours. Histological Typing of Tumours of the Upper Respiratory Tract and Ear. World Health Organization, Springer-Verlag: Berlin
Somers, KD, Winters, BA, Dawson, DM, Leffell, MS, Wright, GL, Devine, CJ, Gilbert, DA & Horton, CE (1987) Chromosome abnormalities in Peyronie’s disease. J Urol 37: 672–675.
Sorensen, PHB, Lessnick, SL, Lopez-Terrada, D, Liu, XF, Triche, TJ & Denny, CT (1994) A second Ewing’s sarcoma translocation, t (21;22), fuses the EWS gene to another ETS-family transcription factor, ERG. Nature Genetics 6: 146–151.
Sorensen, PHB, Wu, JK, Berean, KW, Lim, JF, Donn, W, Frierson, HF, Reynolds, LP, Lopez-Terrada, D & Trichet, J (1996) Olfactory neuroblastoma is a peripheral primitive neuroectodermal tumor related to Ewing sarcoma. Proc Natl Acad Sci USA 93: 1038–1043.
Sreekantaiah, C, Ladanyi, M, Rodriguez, E & Chaganti, RSK (1994) Chromosomal aberrations in soft tissue tumors. Relevance to diagnosis, classification, and molecular mechanisms. Am J Pathol 144: 1121–1133.
Travis, JA & Bridge, JA (1992) Significance of both numerical and structural chromosomal abnormalities in clear cell sarcoma. Cancer Genet Cytogenet 64: 104–106.
VanDevanter, DR, George, D, McNutt, MA, Vogel, A & Luthardt, F (1991) Trisomy 8 in primary esthesioneuroblastoma. Cancer Genet Cytogenet 57: 133–136.
Whang-Peng, J, Freter, CE, Knutsen, T, Nanfro, JJ & Gazdar, A (1987) Translocation t (11;22) in esthesioneuroblastoma. Cancer Genet Cytogenet 29: 155–157.
Wood, GS & Warnke, R (1981) Suppression of endogeneous avidin binding activity in tissues and its relevance to biotin–avidin detection systems. J Histochem Cytochem 29: 1196–1204.
Zucman, J, Delattre, O, Desmaze, C, Plougastel, B, Joubert, I, Melot, T, Peter, M, De Jong, P, Rouleau, G, Aurias, A & Thomas, G (1992) Cloning and characterization of the Ewing’s sarcoma and peripheral neuroepithelioma t (11;22) translocation breakpoint. Genes Chrom Cancer 5: 271–277.
Zucman, J, Melot, T, Desmaze, C, Ghysdael, J, Plougastel, B, Peter, M, Zucker, JM, Triche, TJ, Sheer, D, Turc-Carel, C, Ambros, P, Combaret, V, Lenoir, G, Aurias, A, Thomas, G & Delattre, O (1993) Combinatorial generation of variable fusion proteins in the Ewing family of tumors. EMBO J 12: 4481–4487.
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Mezzelani, A., Tornielli, S., Minoletti, F. et al. Esthesioneuroblastoma is not a member of the primitive peripheral neuroectodermal tumour-Ewing’s group. Br J Cancer 81, 586–591 (1999). https://doi.org/10.1038/sj.bjc.6690734
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DOI: https://doi.org/10.1038/sj.bjc.6690734
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