Summary
Loss of sequences from human chromosome 10q has been reported in several different cancers. Recently, a second candidate tumour-suppressor gene, DMBT1, was identified in this chromosomal region. We studied the mRNA expression and homozygous deletion of this gene in human oesophageal, gastric and colon cancers. Reverse transcriptase polymerase chain reaction (RT-PCR) amplification demonstrated that 23 (53.5%) of 43 oesophageal, 5 (12.5%) of 40 gastric, and 4 (16.7%) of 24 colorectal cancer cases showed an apparent reduction in DMBT1 mRNA in tumour tissues compared with paired normal tissues. Twelve out of 15 oesophageal cancer cell lines also showed no expression. We next studied homozygous deletions within the DMBT1 gene in oesophageal cancers by using duplex PCR. Consequently, it was recognized in five (11.6%) of the primary tumours and two (13.3%) of the cell lines. These findings suggest that DMBT1 may act as a tumour-suppressor gene not only in brain tumours but also in gastrointestinal cancers, especially in oesophageal cancers.
Similar content being viewed by others
Article PDF
Change history
16 November 2011
This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication
References
Albarosa, R., Colombo, B. M., Roz, L., Magnani, I., Pollo, B., Cirenei, N., Giani, C., Conti, A. M., DiDonato, S. & Finocchiaro, G. (1996). Deletion mapping of gliomas suggests the presence of two small regions for candidate tumor-suppressor genes in a 17-cM interval on chromosome 10q. Am J Hum Genet 58: 1260–1267.
Aoki, T., Mori, T., Du, X. O., Nishihira, T., Matsubara, T. & Nakamura, Y. (1994). Allelotype study of esophageal carcinoma. Genes Chroms Cancer 10: 177–182.
Gray, I. C., Phillips, S. M., Lee, S. J., Neoptolemos, J. P., Weissenbach, J. & Spurr, N. K. (1995). Loss of the chromosomal region 10q23–25 in prostate cancer. Cancer Res 55: 4800–4803.
Hahn, S. A., Seymour, A. B., Hoque, A. T. M. S., Schutte, M., da Costa, L. T., Redston, M. S., Caldas, C., Weinstein, C. L., Fischer, A., Yeo, C. J., Hruban, R. H. & Kern, S. E. (1995). Allelotype of pancreatic adenocarcinoma using xenograft enrichment. Cancer Res 55: 4670–4675.
Herbst, R., Weiss, J., Ehnis, A., Cavenee, W. K. & Arden, K. C. (1995). Loss of heterozygosity for 10q22–10qter in malignant melanoma progression. Cancer Res 54: 3111–3114.
Kong, D., Suzuki, A., Zou, T. T., Sakurada, A., Kemp, L. W., Wakatsuki, S., Yokoyama, T., Yamakawa, H., Furukawa, T., Sato, S., Yin, J., Wang, S., Abrahan, J. M., Souza, R. F., Smolinski, K. N., Meltzer, S. J. & Horii, A. (1997). PTEN1 is frequently mutated in primary endometrial carcinomas. Nature Genet 17: 143–144.
Li, J., Mori, M., Yang, Y., Inoue, H., Mimori, K., Shibuta, K., Nakashima, H., Mafune, K., Shimada, Y., Barnard, G. F., Sugimachi, K. & Akiyoshi, T. (1995). Multiple tumor suppressor 1 gene and esophageal carcinoma. Int J Cancer 7: 257–260.
Li, J., Yen, C., Liaw, D., Podsypanina, K., Bose, S., Wang, S. I., Puc, J., Miliaresis, C., Rodgers, L., McCombie, R., Bigner, S. H., Giovanella, B. C., Ittmann, M., Tycko, B., Hibshoosh, H., Wigler, M. H. & Parsons, R. (1997). PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science 275: 1943–1947.
Mollenhauer, J., Wiemann, S., Scheurlen, W., Korn, B., Hayashi, Y., Wilgenbus, K., von Deimling, A. & Poustka, A. (1997). DMBT1, a new member of the SRCR superfamily, on chromosome 10q25.3–26.1 is deleted in malignant brain tumors. Nature Genet 17: 32–39.
Mori, M., Mimori, K., Inoue, H., Barnard, G. F., Tsuji, K., Nanbara, S., Ueo, H. & Akiyoshi, T. (1995). Detection of cancer micrometastases in lymph nodes by reverse transcriptase-polymerase chain reaction. Cancer Res 55: 3417–3420.
Mori, M., Mimori, K., Ueo, H., Tsuji, K., Shiraishi, T., Barnard, G. F., Sugimachi, K. & Akiyoshi, T. (1998). Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcription-polymerase chain reaction in patients with gastrointestinal or breast carcinomas. J Clin Oncol 16: 128–132.
Peiffer, S. L., Herzog, T. J., Tribune, D. J., Mutch, D. G., Gersell, D. J. & Goodfellow, P. J. (1995). Allelic loss of sequences from the long arm of chromosome 10 and replication errors in endometrial cancers. Cancer Res 54: 1922–1926.
Petersen, I., Langreck, H., Wolf, G., Schwendel, A., Psille, R., Vogt, P., Reichel, M. B., Ried, T. & Dietel, M. (1997). Small-cell lung cancer is characterized by a high incidence of deletions on chromosomes 3p, 4q, 5q, 10q, 13q, and 17p. Br J Cancer 75: 79–86.
Author information
Authors and Affiliations
Rights and permissions
From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
About this article
Cite this article
Mori, M., Shiraishi, T., Tanaka, S. et al. Lack of DMBT1 expression in oesophageal, gastric and colon cancers. Br J Cancer 79, 211–213 (1999). https://doi.org/10.1038/sj.bjc.6690035
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.bjc.6690035
Keywords
This article is cited by
-
Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression
Scientific Reports (2017)
-
Detection of deleted in malignant brain tumors 1 and runt-related transcription factor 3 gene expressions in bladder carcinoma
Molecular Biology Reports (2012)
-
DNA copy number profiles of gastric cancer precursor lesions
BMC Genomics (2007)
-
DMBT1 expression is down-regulated in breast cancer
BMC Cancer (2004)
-
Rare mutations of the DMBT1 gene in human astrocytic gliomas
Oncogene (2002)