Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Brief Communication
  • Published:

Longitudinal analysis of B cell repertoire and antibody gene rearrangements during early HIV infection

Genes & Immunity volume 6, pages 66–69 (2005)Cite this article

Abstract

In chronically HIV infected individuals, a number of functional B cell abnormalities have been described. However, the immediate changes that occur in the B cell compartment following viral exposure and how they affect the long-term course of infection are not well understood. We report the longitudinal analysis of B cell repertoires during early infection in untreated and treated individuals receiving highly active antiretroviral therapy (HAART). Analysis was based on IgG heavy chain gene utilization and CDR3 length measurement and relationship with CD4/CD8 counts, viral load, and total serum IgG, and anti-HIV antibodies levels. Repertoires were assessed at baseline and at weeks 2, 4, 12, 24, and 72 after initiation of therapy. The findings indicate a stable peripheral B cell repertoire during the first 72 weeks following infection, particularly in the HAART treated patients. A modest association between B cell repertoire integrity and viremia levels as well as treatment was detected.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1

Similar content being viewed by others

References

  1. Schnittman SM, Lane HC, Higgins SE, Folks T, Fauci AS . Direct polyclonal activation of human B lymphocytes by the acquired immune deficiency syndrome virus. Science 1986; 233: 1084–1086.

    Article  CAS  Google Scholar 

  2. Shirai A, Cosentino M, Leitman-Klinman SF, Klinman DM . Human immunodeficiency virus infection induces both polyclonal and virus-specific B cell activation. J Clin Invest 1992; 89: 561–566.

    Article  CAS  Google Scholar 

  3. David D, Demaison C, Bani L, Zouali M, Theze J . Selective variations in vivo of VH3 and VH1 gene family expression in peripheral B cell IgM, IgD and IgG during HIV infection. Eur J Immunol 1995; 25: 1524–1528.

    Article  CAS  Google Scholar 

  4. Scamurra RW, Miller DJ, Dahl L et al Impact of HIV-1 infection on VH3 gene repertoire of naive human B cells. J Immunol 2000; 164: 5482–5491.

    Article  CAS  Google Scholar 

  5. Chong Y, Ikematsu H, Ariyama I et al. Evidence of B cell clonal expansion in HIV type 1-infected patients. AIDS Res Hum Retroviruses 2001; 17: 1507–1515.

    Article  CAS  Google Scholar 

  6. Malaspina A, Moir S, Kottilil S et al. Deleterious effect of HIV-1 plasma viremia on B cell costimulatory function. J Immunol 2003; 170: 5965–5972.

    Article  CAS  Google Scholar 

  7. Baranzini SE, Jeong MC, Butunoi C, Murray RS, Bernard CC, Oksenberg JR . B cell repertoire diversity and clonal expansion in multiple sclerosis brain lesions. J Immunol 1999; 163: 5133–5144.

    CAS  Google Scholar 

  8. Janssen RS, Satten GA, Stramer SL et al. New testing strategy to detect early HIV-1 infection for use in incidence estimates and for clinical prevention purposes. JAMA 1998; 280: 42–48.

    Article  CAS  Google Scholar 

  9. Machado DM, Delwart EL, Diaz RS et al. Use of the sensitive/less-sensitive (detuned) EIA strategy for targeting genetic analysis of HIV-1 to recently infected blood donors. AIDS 2002; 16: 113–119.

    Article  Google Scholar 

  10. Hecht FM, Busch MP, Rawal B et al. Use of laboratory tests and clinical symptoms for identification of primary HIV infection. AIDS 2002; 16: 1119–1129.

    Article  Google Scholar 

  11. Sansonno D, Lauletta G, De Re V et al. Intrahepatic B cell clonal expansions and extrahepatic manifestations of chronic HCV infection. Eur J Immunol 2004; 34: 126–136.

    Article  CAS  Google Scholar 

  12. Burgoon MP, Owens GP, Smith-Jensen T, Walker G, Gilden DH . Cloning the antibody response in humans with inflammatory central nervous system disease: analysis of the expressed IgG repertoire in subacute sclerosing panencephalitis brain reveals disease relevant antibodies that recognize specific measles virus antigens. J Immunol 1999; 163: 3496–3502.

    CAS  PubMed  Google Scholar 

  13. Pantaleo G, Demarest JF, Soudeyns H et al. Major expansion of CD8+ T cells with predominant V beta usage during primary immune response to HIV. Nature 1999; 379: 463–467.

    Google Scholar 

  14. Schito AM, Vittinghoff E, Hecht FM et al. Longitudinal analysis of T-cell receptor gene use by CD8+ T cells in early human immunodeficiency virus infection in patients receiving highly active antiretroviral therapy. Blood 2001; 97: 214–220.

    Article  CAS  Google Scholar 

  15. Morris L, Binley JM, Clas BA et al. HIV-1 antigen-specific and -nonspecific B cell responses are sensitive to combination antiretroviral therapy. J Exp Med 1998; 188: 233–245.

    Article  CAS  Google Scholar 

  16. Ng VL, McGrath MS . The immunology of AIDS-associated lymphomas. Immunol Rev 1998; 162: 293–298.

    Article  CAS  Google Scholar 

  17. Gaidano G, Capello D, Carbone A . The molecular basis of acquired immunodeficiency syndrome-related lymphomagenesis. Semin Oncol 2000; 27: 431–441.

    CAS  PubMed  Google Scholar 

  18. Loomis-Price LD, Cox JH, Mascola JR et al. Correlation between humoral responses to human immunodeficiency virus type 1 envelope and disease progression in early-stage infection. J Infect Dis 1998; 178: 1306–1316.

    Article  CAS  Google Scholar 

  19. Jennrich RI, Schluchter MD . Unbalanced repeated-measures models with structured covariance matrices. Biometrics 1986; 42: 805–820.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank the study participants. We acknowledge the assistance of Gerald Spotts with database and data management. This study was supported by NIH 1UO1 AI41531, UCSF Center for AIDS Research NIH P30 MH59037.

Author information

Authors and Affiliations

  1. Department of Neurology, University of California at San Francisco, CA, USA

    M K Elkins, S E Baranzini, U Sriram & J R Oksenberg

  2. Department of Epidemiology and Biostatistics, University of California at San Francisco, CA, USA

    E Vittinghoff

  3. Positive Health Program HIV Section, University of California at San Francisco, CA, USA

    F M Hecht

  4. Department of Laboratory Medicine, University of California at San Francisco, CA, USA

    M P Busch

  5. Department of Medicine, University of California at San Francisco, CA, USA

    J A Levy

  6. Blood Centers of the Pacific, Blood Systems Research Institute, San Francisco, CA, USA

    M P Busch

Authors
  1. M K Elkins
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E Vittinghoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S E Baranzini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F M Hecht
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. U Sriram
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M P Busch
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J A Levy
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. J R Oksenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Consortia

For the San Francisco Primary Infection Group

Corresponding author

Correspondence to J R Oksenberg.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Elkins, M., Vittinghoff, E., Baranzini, S. et al. Longitudinal analysis of B cell repertoire and antibody gene rearrangements during early HIV infection. Genes Immun 6, 66–69 (2005). https://doi.org/10.1038/sj.gene.6364146

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.gene.6364146

Keywords

This article is cited by

Search

Advanced search

Quick links