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Proteome analysis of prostate cancer

Prostate Cancer and Prostatic Diseases volume 8, pages 14–21 (2005)Cite this article

Abstract

In this paper, we briefly review cancer proteomics in general, with particular attention to our proteome analyses of prostate cancer. Our efforts include development of new tools and novel approaches to discovering proteins potentially useful as cancer diagnostic and/or prognostic biomarkers or as therapeutic targets. To this end, we analyzed prostate cancer proteomes using two-dimensional gel electrophoresis employing agarose gels for the initial isoelectric focusing step (agarose 2-DE), with mass spectrometry used for protein identification. Agarose 2-DE offers advantages over the more widely used immobilized pH gradient 2-DE for separating high molecular mass proteins (15–500 kDa), thereby increasing its power to detect changes in the cancer's high-molecular mass proteomes.

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Acknowledgements

We thank WA Thomasson, PhD, for expert editorial assistance. This work was supported by Grant-in-Aid for Scientific Research on Priority Areas ‘Medical Genome Science’ from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Urology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan

    H Kuruma & S Egawa

  2. Department of Physics, Kitasato University School of Science, Sagamihara, Kanagawa, Japan

    M Oh-Ishi, Y Kodera & T Maeda

Authors
  1. H Kuruma
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  2. S Egawa
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  3. M Oh-Ishi
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  4. Y Kodera
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  5. T Maeda
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Correspondence to S Egawa.

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Kuruma, H., Egawa, S., Oh-Ishi, M. et al. Proteome analysis of prostate cancer. Prostate Cancer Prostatic Dis 8, 14–21 (2005). https://doi.org/10.1038/sj.pcan.4500764

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