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Altered immunoreactive levels of G proteins in peripheral mononuclear cells of patients with anorexia nervosa and bulimia nervosa

Molecular Psychiatry volume 8, pages 680–684 (2003)Cite this article

Abstract

Alterations of cellular G proteins have been implicated in the pathophysiology of some psychiatric disorders. So far, no study assessed G protein function in anorexia nervosa (AN) and bulimia nervosa (BN). Therefore, we measured immunoreactive levels of Gαs, Gαi, Gαq/11 and Gβ protein subunits in mononuclear leukocytes of 71 drug-free women, including 25 subjects with AN, 26 individuals with BN and 20 healthy controls. As compared to healthy women, anorexic patients exhibited significantly increased levels of Gαi and Gβ proteins, while bulimic patients had significantly increased levels of Gαs, Gαi and Gβ proteins. Immunoreactive levels of peripheral G protein subunits were not significantly correlated with demographic or nutritional parameters. These findings, although obtained in peripheral blood cells, may suggest a derangement of G protein-mediated signal transduction in the pathophysiology of eating disorders.

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References

  1. Spiegel AM, Shenker A, Weinstein LS . Receptor–effector coupling by G proteins: implications for normal and abnormal signal transduction. Endocr Rev 1992; 13: 536–565.

    Article  CAS  Google Scholar 

  2. Manji HK . G proteins: implications for psychiatry. Am J Psychiatry 1992; 149: 746–760.

    Article  CAS  Google Scholar 

  3. Schreiber G, Avissar S, Danon A, Belmaker RH . Hyperfunctional G proteins in mononuclear leukocytes of patients with mania. Biol Psychiatry 1991; 29: 273–280.

    Article  CAS  Google Scholar 

  4. Avissar S, Nechamkin Y, Barki-Harrington L, Roitman G, Schreiber G . Differential G protein measures in mononuclear leukocytes of patients with bipolar mood disorder are state dependent. J Affect Disord 1997; 43: 85–93.

    Article  CAS  Google Scholar 

  5. Avissar S, Nechamkin Y, Roitman G, Schreiber G . Reduced G protein functions and immunoreactive levels in mononuclear leukocytes of patients with depression. Am J Psychiatry 1997; 154: 211–217.

    Article  CAS  Google Scholar 

  6. Young LT, Li PP, Kamble A, Siu KP, Warsh JJ . Mononuclear leukocyte levels of G proteins in depressed patients with bipolar disorder or major depressive disorder. Am J Psychiatry 1994; 151: 594–596.

    Article  CAS  Google Scholar 

  7. Manji HK, Chen G, Potter W, Kosten TR . Guanine nucleotide binding proteins in opioid-dependent patients. Biol Psychiatry 1997; 41: 130–134.

    Article  CAS  Google Scholar 

  8. Okada F . G proteins in the medial temporal lobe in schizophrenia. In: Boulton AA, Baker GB (series eds), Neuromethods, Vol 31: Mishra RK, Baker GB, Boulton AA (eds) G Protein Methods and Protocols Humana Press Inc.: Totowa, 1997, 379–391.

    Google Scholar 

  9. Jope RS, Song L, Grimes CA, Pacheco MA, Dilley GE, Li X et al Selective increases in phosphoinositide signalling activity and g protein levels in postmortem brain from subjects with schizophrenia or alcohol dependence. J Neurochem 1998; 70: 763–771.

    Article  CAS  Google Scholar 

  10. Avissar S, Barki-Harrington L, Nechamkin Y, Roitman G, Schreiber G . Elevated dopamine receptor-coupled Gs protein measures in mononuclear leukocytes of patients with schizophrenia. Schizophrenia Res 2001; 47: 37–47.

    Article  CAS  Google Scholar 

  11. Stein MB, Chen G, Potter WZ, Manji HK . G-protein level quantification in platelets and leukocytes from patients with panic disorder. Neuropsychopharmacology 1996; 15: 180–186.

    Article  CAS  Google Scholar 

  12. First MB, Spitzer RL, Gibbon M, Williams JB . Structured Clinical Interview for DSM-IV Axis I Disorders—Patient Edition. SCID-I/P, Version 2). Biometrics Research Department: New York, 1995.

    Google Scholar 

  13. Andreasen NC, Endicott J, Spitzer RL, Winokur G . The family history method using diagnostic criteria: reliability and validity. Arch Gen Psychiatry 1977; 34: 1229–1235.

    Article  CAS  Google Scholar 

  14. Garner DL, Olmstead MP, Polivy J . Development and validation of a multidimensional eating disorders inventory for anorexia nervosa and bulimia. Int J Eat Disord 1983; 2: 15–35.

    Article  Google Scholar 

  15. Henderson M, Freeman CPL . A self-rating scale for bulimia: the BITE. Br J Psychiatry 1987; 150: 18–24.

    Article  CAS  Google Scholar 

  16. Bradford MM . A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein–dye binding. Ann Biochem 1976; 2: 248–254

    Article  Google Scholar 

  17. Dixon J . BMDP Statistical Software. University of California Press: Berkeley, 1985.

    Google Scholar 

  18. Klein S, Luu K, Gasic S, Green A . Effect of weight loss on whole body and cellular lipid metabolism in severely obese humans. Am J Physiol 1996; 270: E739–E745.

    CAS  PubMed  Google Scholar 

  19. Saito N, Guitart X, Hayward M, Tallsman JF, Duman RS, Nestler EJ . Corticosterone differentially regulates the expression of Gsα and Giα messenger RNA and protein in rat cerebral cortex. Proc Natl Acad Sci USA 1989; 86: 3906–3910.

    Article  CAS  Google Scholar 

  20. Bouvier C, Lagacié G, Collu R . G protein modulation by estrogens. Mol Cell Endocrinol 1991; 79: 65–73.

    Article  CAS  Google Scholar 

  21. Milligan G, Spiegel AM, Unson CG, Saggerson ED . Chemically induced hypothyroidism produces elevated amounts of the α subunit of the inhibitory guanine nucleotide binding protein (Gi) and the β subunit common to all G-proteins. Biochem J 1987; 247: 223–227.

    Article  CAS  Google Scholar 

  22. Stoving RK, Hangaard J, Hansen-Nord M, Hagen C . A review of endocrine changes in anorexia nervosa. J Psychiatr Res 1999; 33: 139–152.

    Article  CAS  Google Scholar 

  23. Monteleone P, Brambilla F, Bortolotti F, Maj M . Serotoninergic dysfunction across the eating disorders: relationship to eating behaviour, purging behaviour, nutritional status and general psychopathology. Psychol Med 2000; 30: 1099–1110.

    Article  CAS  Google Scholar 

  24. Livnat S, Felten SY, Carlson SL, Bellingher DL, Felten DL . Involvement of peripheral and central catecholamine systems in neural immune interactions. J Neuroimmunol 1985; 10: 5–30.

    Article  CAS  Google Scholar 

  25. Rechlin T, Weis M, Ott C, Bleichner F, Joraschky P . Alterations of autonomic cardiac control in anorexia nervosa. Biol Psychiatry 1998; 43: 358–363.

    Article  CAS  Google Scholar 

  26. Pirke KM . Central and peripheral noradrenalin regulation in eating disorders. Psychiatry Res 1996; 62: 43–49.

    Article  CAS  Google Scholar 

  27. Garcia-Sevilla JA, Walzer C, Busquets X, Escribà PV, Balant L, Guimon J . Density of guanine nucleotide-binding proteins in platelets of patients with major depression: increased abundance of the Gαi2 subunit and down-regulation by antidepressant drug treatment. Biol Psychiatry 1997; 42: 704–712.

    Article  CAS  Google Scholar 

  28. Wand GS, Waltman C, Martin CS, McCaul ME, Levine MA, Wolfang D . Differential expression of guanosine triphosphate binding proteins in men at high and low risk for the future development of alcoholism. J Clin Invest 1994; 94: 1004–1011.

    Article  CAS  Google Scholar 

  29. Manji HK, Chen G, Shimon H, Hsiao JK, Potter WZ, Belmaker RH . Guanine nucleotide-binding proteins in bipolar affective disorder: effects of long-term lithium treatment. Arch Gen Psychiatry 1995; 52: 135–144.

    Article  CAS  Google Scholar 

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  1. Department of Psychiatry, University of Naples S.U.N., Naples, Italy

    P Monteleone, A Di Lieto, V Martiadis, M Pannuto & M Maj

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  1. P Monteleone
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Correspondence to P Monteleone.

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Monteleone, P., Di Lieto, A., Martiadis, V. et al. Altered immunoreactive levels of G proteins in peripheral mononuclear cells of patients with anorexia nervosa and bulimia nervosa. Mol Psychiatry 8, 680–684 (2003). https://doi.org/10.1038/sj.mp.4001304

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