Abstract
Endothelial dysfunction or activation elicited by oxidatively modified low-density lipoprotein (Ox-LDL) has been implicated in the pathogenesis of atherosclerosis1–4, characterized by intimal thickening and lipid deposition in the arteries. Ox-LDL and its lipid constituents impair endothelial production of nitric oxide, and induce the endothelial expression of leukocyte adhesion molecules and smooth-muscle growth factors, which may be involved in atherogenesis5–7. Vascular endothelial cells in culture8,9 and in vivo10,11 internalize and degrade Ox-LDL through a putative receptor-mediated pathway that does not involve macrophage scavenger receptors12–15. Here we report the molecular cloning, using expression cloning strategy, of an Ox-LDL receptor from vascular endothelial cells. The cloned receptor is a membrane protein that belongs structurally to the C-type lectin family, and is expressed in vivo in vascular endothelium and vascular-rich organs.
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References
Brown, M. S. & Goldstein, J. L. Annu. Rev. Biochem. 52, 223–261 (1983).
Steinberg, D., Parthasarathy, S., Carew, T. E., Khoo, J. C. & Witztum, J. L. N. Engl. J. Med. 320, 915–924 (1989).
Kita, T. et al. Proc. Natl Acad. Sci. USA 84, 5928–5931 (1987).
Ross, R. Nature 368, 801–809 (1993).
Gimbrone, M. A. J., Cybulsky, M. I., Kume, N., Collins, T. & Resnick, N. Ann. NY Acad. Sci. 748, 122–132 (1995).
Kume, N. & Gimbrone, M. A. J. J. Clin. Invest. 93, 907–911 (1994).
Kugiyama, K., Kerns, S. A., Morrisett, J. D., Roberts, R. & Henry, P. D. Nature 344, 160–162 (1990).
Stein, O. & Stein, Y. Biochim. Biophys. Acta 620, 631–635 (1980).
Kume, N., Arai, H., Kawai, C. & Kita, T. Biochim. Biophys. Acta 1091, 63–67 (1991).
Pitas, R. E., Boyles, J., Mahley, R. W. & Bissel, D. M. J. Cell. Biol. 100, 103–117 (1985).
van Berkel, T. J. C., De Rijke, Y. B. & Kruijt, J. K. J. Biol. Chem. 266, 2282–2289 (1991).
Krieger, M. et al. J. Biol. Chem. 268, 4569–4572 (1993).
Kodama, T. et al. Nature 343, 531–535 (1990).
Rohrer, L., Freeman, M., Kodama, T., Penman, M. & Krieger, M. Nature 343, 570–572 (1990).
Bickel, P. E. & Freeman, M. W. J. Clin. Invest. 90, 1450–1457 (1992).
Drickamer, K. J. J. Biol. Chem. 263, 9557–9560 (1988).
Giorda, R. et al. Science 249, 1298–1300 (1990).
Endemann, G. et al. J. Biol. Chem. 268, 11811–11816 (1993).
Acton, S. L., Scherer, P. E., Lodish, H. F. & Krieger, M. J. Biol. Chem. 269, 21003–21009 (1994).
Standon, L. W., White, R. T., Bryant, C. M., Protter, A. A. & Endemann, G. J. Biol. Chem. 267, 22446–22451 (1992).
Ramprasad, M. P. et al. Proc. Natl Acad. Sci. USA 92, 9580–9584 (1995).
Fraser, I., Hughes, D. & Gordon, S. Nature 364, 343–346 (1993).
Show, G. & Kamen, R. Cell 46, 659–667 (1986).
Stephan, Z. F. & Yurachek, E. C. J. Lipid Res. 34, 325–330 (1993).
Ikura, T. et al. Biochem. Biophys. Res. Commun. 203, 1417–1422 (1994).
Hirt, B. J. Mol. Biol. 26, 365–369 (1967).
Frohman, M. A., Dush, M. K. & Martin, G. R. Proc. Natl Acad. Sci. USA 85, 8998–9002 (1988).
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Sawamura, T., Kume, N., Aoyama, T. et al. An endothelial receptor for oxidized low-density lipoprotein. Nature 386, 73–77 (1997). https://doi.org/10.1038/386073a0
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DOI: https://doi.org/10.1038/386073a0
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