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Simplified hot start PCR

The use of a thermally activated DNA polymerase PCR gives improved specificity, sensitivity and product yield without additives or extra process steps.

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References

  1. Faloona, F. S., Weiss, S., Ferre, F. & Mullis, K. 6th Int'l Conf. AIDS, San Francisco, CA, USA (abstract No. 1019) (1990).

  2. Chou, Q., Russell, M., Birch, D. E., Raymond, J. & Bloch, W. Nucleic Acids Res. 20, 1717–1723 (1992).

    Article  CAS  Google Scholar 

  3. Horton, R. M., Hoppe, B. L. & Conti-Tronconi, B. M. BioTechniques 16, 42–43 (1994).

    CAS  PubMed  Google Scholar 

  4. Kellogg, D. E. et al. BioTechniques 16, 1134–1137 (1994).

    CAS  PubMed  Google Scholar 

  5. Findlay, J. B. et al. Clin. Chem. 39, 1927–1933 (1993).

    CAS  PubMed  Google Scholar 

  6. Kwok, S., Gates, C., Castro, R. & Sninsky, J. J. 92nd Gen. Mtg. Am. Soc. Microbiol. (abstract No. D-120) (1992).

  7. Lynch, C. E., Madej, R., Louie, P. H. & Rodgers, G. J. Acquir. Immune. Defic. Syndr. 5, 433–440 (1992).

    CAS  Google Scholar 

  8. Tevere, V. J. et al. J. Clin. Microbiol. (In Press)

  9. Jin, D. J. & Gross, C. A. J. molec. Biol. 202, 45–58. (1988)

    Article  CAS  Google Scholar 

  10. Lawyer, F. C. et al. J. biol. Chem. 264, 6427–6437 (1989).

    CAS  Google Scholar 

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Birch, D., Kolmodin, L., Wong, J. et al. Simplified hot start PCR. Nature 381, 445–446 (1996). https://doi.org/10.1038/381445a0

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