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Characterization and inhibition of a cholecystokinin-inactivating serine peptidase

Abstract

A cholecystokinin (CCK)-inactivating peptidase was purified and identified as a membrane-bound isoform of tripeptidyl peptidase II (EC 3.4.14.10), a cytosolic subtilisin-like peptidase of previously unknown functions. The peptidase was found in neurons responding to cholecystokinin, as well as in non-neuronal cells. Butabindide, a potent and specific inhibitor, was designed and shown to protect endogenous cholecystokinin from inactivation and to display pro-satiating effects mediated by the CCKA receptor.

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Rose, C., Vargas, F., Facchinetti, P. et al. Characterization and inhibition of a cholecystokinin-inactivating serine peptidase. Nature 380, 403–409 (1996). https://doi.org/10.1038/380403a0

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