Letter

Sheep cloned by nuclear transfer from a cultured cell line

  • Nature volume 380, pages 6466 (07 March 1996)
  • doi:10.1038/380064a0
  • Download Citation
Received:
Accepted:
Published:

Abstract

NUCLEAR transfer has been used in mammals as both a valuable tool in embryological studies1 and as a method for the multiplication of 'elite' embryos2–4. Offspring have only been reported when early embryos, or embryo-derived cells during primary culture, were used as nuclear donors5,6. Here we provide the first report, to our knowledge, of live mammalian offspring following nuclear transfer from an established cell line. Lambs were born after cells derived from sheep embryos, which had been cultured for 6 to 13 passages, were induced to quiesce by serum starvation before transfer of their nuclei into enucleated oocytes. Induction of quiescence in the donor cells may modify the donor chromatin structure to help nuclear reprogramming and allow development. This approach will provide the same powerful opportunities for analysis and modification of gene function in livestock species that are available in the mouse through the use of embryonic stem cells7.

  • Subscribe to Nature for full access:

    $199

    Subscribe

Additional access options:

Already a subscriber?  Log in  now or  Register  for online access.

References

  1. 1.

    & Science 220, 1300–1302 (1983).

  2. 2.

    , & Therio 33, 165–174 (1990).

  3. 3.

    & Int. Rev. Cytol. 120, 169–190 (1990).

  4. 4.

    , , & C.r. Acad. Sci. Paris Life Sci. 316, 487–491 (1993).

  5. 5.

    & Proc. natn. Acad. Sci. U.S.A. 91, 6243–6147 (1994).

  6. 6.

    & Molec. Repr. Dev. 38, 264–267 (1994).

  7. 7.

    Embryonal Stem Cells: Introducing Planned Changes into the Germline (ed. Evans, H. J.) (Harwood Academic, Switzerland, 1992).

  8. 8.

    , , & Zygote 2, 385–389 (1994).

  9. 9.

    , & Biol. Reprod. 49, 933–942 (1993).

  10. 10.

    , , & Biol. Reprod. 50, 1385–1393 (1994).

  11. 11.

    , , & J. Cell Sci. 91, 603–613 (1988).

  12. 12.

    & J. Reprod. Fertil. 17, 399–401 (1968).

  13. 13.

    & J. Am. Stat. Assoc. 88, 9–25 (1993).

  14. 14.

    , , & Control of Animal Cell Proliferation Vol. 1 (eds Boynton, A. L. & Leffert, H. L.) 331–365 (Academic, London 1985).

  15. 15.

    , & Therio 34, 879–901 (1990).

  16. 16.

    & Anim. Biotechnol. 6, 1–14 (1995).

  17. 17.

    Nature 320, 63–65 (1986).

  18. 18.

    , & Anim. Genet. 25, 60 (1994).

Download references

Author information

Affiliations

  1. Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, UK

    • K. H. S. Campbell
    • , J. McWhir
    • , W. A. Ritchie
    •  & I. Wilmut

Authors

  1. Search for K. H. S. Campbell in:

  2. Search for J. McWhir in:

  3. Search for W. A. Ritchie in:

  4. Search for I. Wilmut in:

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.