Abstract
THE myogenic basic helix–loop–helix transcription factors, Myf5, MyoD, myogenin and MRF4, play key roles in skeletal muscle development1,2. All of them induce myogenic differentiation in cultured non-muscle cells, suggesting that they might be functionally redundant. But the genes are expressed at different times during embryogenesis3–6 and mice carrying a mutation in any of the genes have different phenotypes7–13. A rib cage defect was observed in Myf5-deficient mice, which die perinatally7. We investigated whether the rib cage defect was due to the failure of the early activation of the gene or to the unique interactions of Myf5 with specific downstream targets. For this we inserted a myogenin complementary DNA into the Myf5 locus by homologous recombination which simultaneously disrupted Myf5 function. We report here that mice homozygous for this myogenin gene knock-in (ki) developed a normal rib cage and were viable, therefore demonstrating functional redundancy of Myf5 and myogenin for rib formation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Olson, E. N. & Klein, W. H. Genes Dev. 8, 1–8 (1994).
Rudnicki, M. A. & Jaenisch, R. Bioessays 17, 203–209 (1995).
Ott, M.-O., Bober, E., Lyons, G., Arnold, H. H. & Buckingham, M. Development 111, 1097–1107 (1991).
Sassoon, D. et al. Nature 341, 303–307 (1989).
Hinterberger, T., Sassoon, D., Rhodes, S. & Koneiczny, S. Devl. Biol. 147, 144–156 (1991).
Bober, E. et al. J. Cell Biol. 113, 1255–1265 (1991).
Braun, T., Rudnicki, M. A., Arnold, H.-H. & Jaenisch, R. Cell 71, 369–382 (1992).
Rudnicki, M. A., Braun, T., Hinuma, S. & Jaenisch, R. Cell 71, 383–390 (1992).
Hasty, P. et al. Nature 363, 501–506 (1993).
Nabeshima, Y. et al. Nature 364, 532–535 (1993).
Braun, T. & Arnold, H. H. EMBO J. 14, 1176–1186 (1995).
Zhang, W., Boeringer, R. R. & Olson, E. N. Genes Dev. 9, 1388–1399 (1995).
Patapoutian, A. et al. Development 121, 3347–3358 (1995).
Weintraub, H. et al. Science 251, 761–766 (1991).
Gu, H., Zou, Y. R. & Rajewsky, K. Cell 78, 1155–1164 (1993).
Hanks, M., Wurst, W., Anson-Carwright, L., Auerbach, A. B. & Joyner, A. L. Science 269, 679–682 (1995).
Rudnicki, M. A. et al. Cell 75, 1241–1244 (1993).
Tajbakhsh, S. & Buckingham, M. E. Proc. natn. Acad. Sci. U.S.A. 91, 747–751 (1994).
Li, E., Bestor, T. H. & Jaenisch, R. Cell 69, 915–926 (1992).
Wright, W. E., Binder, M. & Funk, W. Molec. cell. Biol. 11, 4104–4110 (1991).
Hollenberg, S. M., Cheng, P. E. & Weintraub, H. Proc. natn. Acad. Sci. U.S.A. 90, 8028–8032 (1993).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wang, Y., Schnegelsberg, P., Dausman, J. et al. Functional redundancy of the muscle-specific transcription factors Myf5 and myogenin. Nature 379, 823–825 (1996). https://doi.org/10.1038/379823a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/379823a0
This article is cited by
-
Eight SNPs of the Myf5 gene and diplotypes associated with growth and reproductive traits in Jinghai yellow chicken
Molecular Biology Reports (2014)
-
Binding of carbon nanotube to BMP receptor 2 enhances cell differentiation and inhibits apoptosis via regulating bHLH transcription factors
Cell Death & Disease (2012)
-
Effect of the g.–723G→T Polymorphism in the Bovine Myogenic Factor 5 (Myf5) Gene Promoter Region on Gene Transcript Level in the Longissimus Dorsi Muscle and on Meat Traits of Polish Holstein-Friesian Cattle
Biochemical Genetics (2010)
-
Myf5 −/− :MyoD −/− amyogenic fetuses reveal the importance of early contraction and static loading by striated muscle in mouse skeletogenesis
Development Genes and Evolution (2006)
-
Characterization of Muscle-Regulatory Gene, MyoD, from Flounder (Paralichthys olivaceus) and Analysis of Its Expression Patterns During Embryogenesis
Marine Biotechnology (2006)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.