Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Protein folding in the central cavity of the GroEL–GroES chaperonin complex

Nature volume 379, pages 420–426 (1996)Cite this article

Abstract

The chaperonin GroEL is able to mediate protein folding in its central cavity. GroEL-bound dihydrofolate reductase assumes its native conformation when the GroES cofactor caps one end of the GroEL cylinder, thereby discharging the unfolded polypeptide into an enclosed cage. Folded dihydrofolate reductase emerges upon ATP-dependent GroES release. Other proteins, such as rhodanese, may leave GroEL after having attained a conformation that is committed to fold. Incompletely folded polypeptide rebinds to GroEL, resulting in structural rearrangement for another folding trial in the chaperonin cavity.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Hemmingsen, S. M. et al. Nature 333, 330–334 (1988).

    Article  ADS  CAS  Google Scholar 

  2. Hendrick, J. P. & Hartl, F. U. A. Rev. Biochem. 62, 349–384 (1993).

    Article  CAS  Google Scholar 

  3. Ellis, R. J. Opin. struct. Biol. 4, 117–122 (1994).

    Article  CAS  Google Scholar 

  4. Landry, S. J. & Gierasch, L. M. A. Rev. Biophys. biomol. Struct. 23, 645–669 (1994).

    Article  CAS  Google Scholar 

  5. Langer, T., Pfeifer, G., Martin, J., Baumeister, W. & Hartl, F. U. EMBO J. 11, 4757–4765 (1992).

    Article  CAS  Google Scholar 

  6. Creighton, T. E. Nature 352, 17–18 (1991).

    Article  ADS  CAS  Google Scholar 

  7. Braig, K., Simon, M., Furuya, F., Hainfeld, J. F. & Horwich, A. L. Proc. natn. Acad. Sci. U.S.A. 90, 3978–3982 (1993).

    Article  ADS  CAS  Google Scholar 

  8. Chen, S. et al. Nature 371, 261–264 (1994).

    Article  ADS  CAS  Google Scholar 

  9. Martin, J. et al. Nature 352, 36–42 (1991).

    Article  ADS  CAS  Google Scholar 

  10. Hayer-Hartl, M. K., Ewbank, J. J., Creighton, T. E. & Hartl, F. U. EMBO J. 13, 3192–3202 (1994).

    Article  CAS  Google Scholar 

  11. Zahn, R., Spitzfaden, C., Ottiger, M., Wuthrich, K. & Pluckthun, A. Nature 368, 261–265 (1994).

    Article  ADS  CAS  Google Scholar 

  12. Robinson, C. V. et al. Nature 372, 646–651 (1994).

    Article  ADS  CAS  Google Scholar 

  13. Braig, K. et al. Nature 371, 578–586 (1994).

    Article  ADS  CAS  Google Scholar 

  14. Fenton, W. A., Kashi, Y., Furtak, K. & Horwich, A. L. Nature 371, 615–619 (1994).

    Article  ADS  Google Scholar 

  15. Saibil, H., Dong, Z., Wood, S. & auf der Mauer, A. Nature 353, 25–26 (1991).

    Article  ADS  CAS  Google Scholar 

  16. Engel, A. et al. Science 269, 832–836 (1995).

    Article  ADS  CAS  Google Scholar 

  17. Hayer-Hartl, M. K., Martin, J. & Hartl, F. U. Science 269, 836–841 (1995).

    Article  ADS  CAS  Google Scholar 

  18. Martin, J., Mayhew, M., Langer, T. & Hartl, F. U. Nature 366, 228–233 (1993).

    Article  ADS  CAS  Google Scholar 

  19. Todd, M. J., Viitanen, P. V. & Lorimer, G. H. Science 265, 659–666 (1994).

    Article  ADS  CAS  Google Scholar 

  20. Burston, S. G., Ranson, N. A. & Clarke, A. R. J. molec. Biol. 249, 138–152 (1995).

    Article  CAS  Google Scholar 

  21. Hendrick, J. P. & Hartl, F. U. FASEB J. 9, 1559–1569 (1995).

    Article  CAS  Google Scholar 

  22. Bochkareva, E. S. & Girshovich, A. S. J. biol. Chem. 269, 23869–23871 (1994).

    CAS  PubMed  Google Scholar 

  23. Gray, T. E. & Fersht, A. R. FEBS Lett. 292, 254–258 (1991).

    Article  CAS  Google Scholar 

  24. Weissman, J. S., Kashi, Y., Fenton, W. A. & Horwich, A. L. Cell 78, 693–702 (1994).

    Article  CAS  Google Scholar 

  25. Saibil, H. R. et al. Curr. Biol. 3, 265–273 (1993).

    Article  CAS  Google Scholar 

  26. Gray, T. E. & Fersht, A. R. J. molec. Biol. 232, 1197–1207 (1993).

    Article  CAS  Google Scholar 

  27. Corrales, F. J. & Fersht, A. R. Proc. natn. Acad. Sci. U.S.A. 92, 5326–5330 (1995).

    Article  ADS  CAS  Google Scholar 

  28. Ranson, N. A., Dunster, N. J., Burston, S. G. & Clarke, A. R. J. molec. Biol. 250, 581–586 (1995).

    Article  CAS  Google Scholar 

  29. Touchette, N., Perry, K. & Matthews, C. R. Biochemistry 25, 5445–5452 (1986).

    Article  CAS  Google Scholar 

  30. Frieden, C. Proc. natn. Acad. Sci. U.S.A. 87, 4413–4416 (1990).

    Article  ADS  CAS  Google Scholar 

  31. Ostermann, J., Horwich, A. L., Neupert, W. & Hartl, F. U. Nature 341, 125–130 (1989).

    Article  ADS  CAS  Google Scholar 

  32. Rassow, J. et al. Molec. cell. Biol. 15, 2654–2662 (1995).

    Article  CAS  Google Scholar 

  33. Hummel, J. P. & Dryer, W. J. Biochim. biophys. Acta 63, 530–532 (1962).

    Article  CAS  Google Scholar 

  34. Jackson, G. S. et al. Biochemistry 32, 2554–2563 (1993).

    Article  CAS  Google Scholar 

  35. Creighton, T. E. Biochem. J. 270, 1–16 (1990).

    Article  CAS  Google Scholar 

  36. Dobson, C. M., Evans, P. A. & Radford, S. E. Trends biochem. Sci. 19, 31–37 (1994).

    Article  CAS  Google Scholar 

  37. Horowitz, P. M. & Criscimagna, N. L. J. biol. Chem. 265, 2576–2583 (1990).

    CAS  PubMed  Google Scholar 

  38. Horwich, A. L., Low, K. B., Fenton, W. A., Hirshfield, I. N. & Furtak, K. Cell 74, 909–917 (1993).

    Article  CAS  Google Scholar 

  39. Hartl, F. U. Nature 371, 557–559 (1994).

    Article  ADS  CAS  Google Scholar 

  40. Landry, S. J., Zeilstra, R. J., Fayet, O., Georgopoulos, C. & Gierasch, L. M. Nature 364, 255–258 (1993).

    Article  ADS  CAS  Google Scholar 

  41. Zimmerman, S. B. & Trach, S. O. J. molec. Biol. 222, 599–620 (1991).

    Article  CAS  Google Scholar 

  42. Higuchi, R. in PCR Protocols (eds Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J.) 177–183 (Academic, San Diego, CA, 1990).

    Google Scholar 

  43. Pratt, J. M. In Transcription and Translation: A practical approach (eds Harnes, B. D. & Higgins, S. J.) 179–210 (IRL, Oxford, 1984).

    Google Scholar 

  44. Lissin, N. M., Venyaminov, S. & Girshovich, A. S. Nature 348, 339–342 (1990).

    Article  ADS  CAS  Google Scholar 

  45. Schägger, M. & von Jagow, G. Analyt. Biochem. 166, 368–379 (1987).

    Article  Google Scholar 

  46. Tempst, P., Geromanos, S., Elicone, C. & Erdjument-Bromage, H. Metnods: A Companion to Methods in Enzymology 6, 248–261 (1994).

    CAS  Google Scholar 

  47. Hlodan, R., Tempst, P. & Hartl, F. U. Nature struct. Biol. 2, 587–595 (1995).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Jörg Martin

    Present address: Department of Molecular and Cell Biology and Biochemistry, Brown University, Box G-J2, Providence, Rhode Island, 02912, USA

Authors and Affiliations

  1. Cellular Biochemistry and Biophysics Program and Howard Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York, 10021, USA

    Mark Mayhew, Ana C. R. da Silva, Jörg Martin & F. Ulrich Hartl

  2. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York, 10021, USA

    Hediye Erdjument-Bromage & Paul Tempst

Authors
  1. Mark Mayhew
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ana C. R. da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jörg Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hediye Erdjument-Bromage
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Paul Tempst
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. F. Ulrich Hartl
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mayhew, M., da Silva, A., Martin, J. et al. Protein folding in the central cavity of the GroEL–GroES chaperonin complex. Nature 379, 420–426 (1996). https://doi.org/10.1038/379420a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/379420a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing