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A giant nucleopore protein that binds Ran/TC4

Nature volume 376, pages 184–188 (1995)Cite this article

Abstract

RAN/TC4 is a small nuclear G protein1 that forms a complex with the chromatin-bound guanine nucleotide release factor RCC1 (ref. 2). Loss of RCC1 causes defects in cell cycle progression3,4, RNA export5-7 and nuclear protein import8. Some of these can be suppressed by overexpression of Ran/TC4 (ref. 1), suggesting that Ran/TC4 functions downstream of RCC1. We have searched for proteins that bind Ran/TC4 by using a two-hybrid screen, and here we report the identification of RanBP2, a novel protein of 3,224 residues. This giant protein comprises an amino-terminal 700-residue leucine-rich region, four RanBPl-homologous (refs 9, 10) domains, eight zinc-finger motifs similar to those of NUP153 (refs 11, 12), and a carboxy terminus with high homology to cyclophilin13. The molecule contains the XFXFG pentapeptide motif characteristic of nuclear pore complex (NPC) proteins14, and immunolocalization suggests that RanBP2 is a constituent of the NPC. The fact that NLS-mediated nuclear import can be inhibited by an antibody directed against RanBP2 supports a functional role in protein import through the NPC.

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Authors and Affiliations

  1. Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-82, Japan

    N. Yokoyama, N. Hayashi, T. Seki, T. Ohba, T. Hayashida & T. Nishimoto

  2. Department of Neurosurgery, Graduate School of Medical Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-82, Japan

    N. Yokoyama & M. Fukui

  3. Department of Anatomy, Graduate School of Medical Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-82, Japan

    K. Nishii

  4. Department of Biophysics, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto, 606, Japan

    K. Kuma & T. Miyata

  5. M. E. Müller Institute for Microscopy, Biozentrum, University of Basel, CH-4056, Basel, Switzerland

    N. Panté & U. Aebi

  6. Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205, USA

    U. Aebi

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  1. N. Yokoyama
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Yokoyama, N., Hayashi, N., Seki, T. et al. A giant nucleopore protein that binds Ran/TC4. Nature 376, 184–188 (1995). https://doi.org/10.1038/376184a0

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