Abstract
IN virtually all eukaryotes, mitosis starts after the completion of DNA synthesis. This orderly process is ensured by the checkpoint mechanism that blocks the onset of mitosis while DNA is being synthesized or is damaged. In the fission yeast Schizosaccharomyces pombe, this mechanism involves some rad+ and hus+ genes1 –4. However, it is not known how the checkpoint system monitors these events. Recently a multicopy suppressor of a temperature-sensitive DNA polymerase-α mutant was isolated. This gene, namedcds1+ (checking DNA synthesis), encodes a typical protein kinase. Here we report that this protein kinase is a key component of the DNA replication-monitoring S/G2 checkpoint system. Our data suggest that its primary role is to monitor DNA synthesis by interacting with DNA polymerase a and send a signal to block the onset of mitosis while DNA synthesis is in progress.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Rowley, R., Subramani, S. & Young, P. G. EMBO J. 11, 1335–1342 (1992).
Al-Khodairy, F. & Carr, A. M. EMBO J. 11, 1343–1350 (1992).
Enoch, T., Carr, A. M. & Nurse, P. Genes Dev. 6, 2035–2046 (1992).
Al-Khodairy et al. Molec. Biol. Cell 5, 147–160 (1994).
Damagnez, V., Tillt, J., deRecondo, A. M. & Baldacci, G. Molec. Gen. Genet. 226, 182–189 (1991).
Singh, J. & Klar, A. J. S. Nature 361, 271–273 (1993).
Stern, D. F. P., Zheng, P., Beidler, D. R., & Zerillo, C. Molec. cell. Biol. 11, 987–1001 (1991).
Zheng, P. et al. Molec. cell. Biol. 9, 5829–5842 (1993).
Weinert, T. A., Kiser, G. L. & Hartwell, L. H. Genes Dev. 8, 652–665 (1994).
Allen, J. B., Zhou, Z., Siede, W., Friedberg, E. C. & Elledge, S. J. Genes Dev. 8, 2416–2428 (1994).
Sunnerhagen, P., Seatron, B. L., Nasim, A. & Subramani, S. Molec. cell. Biol. 10, 3750–3760 (1990).
Murray, J. M., Carr, A. M., Lehmann, A. R. & Watts, F. Z. Nucleic Acids Res. 19, 3525–3541 (1991).
Walworth, N., Davey, S. & Beach, D. Nature 363, 368–371 (1993).
Fantes, P. Nature 279, 428–430 (1979).
Enoch, T. & Nurse, P. Cell 60, 665–673 (1990).
Hirano, T., Funahashi, S., Uemura, T. & Yanagida, M. EMBO J. 5, 2973–2979 (1986).
Sazer, S. & Nurse, P. EMBO J. 13, 606–615 (1994).
Sarabia, M.-J. F., McInerny, C., Harris, P., Gordon, C. & Fantes, P. Molec. Gen. Genet. 238, 241–251 (1993).
Nasmyth, K. A. Cell 12, 101–106 (1977).
Peck, V. M., Gerner, E. W. & Cress, A. E. Biochem. biophys. Res. Commun. 190, 325–331 (1993).
Tanaka, K. et al. EMBO J. 11, 4923–4932 (1992).
Sanger, F., Nicklen, S., Barrel, B. G., Smith, A. J. H. & Roe, B. A. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murakami, H., Okayama, H. A kinase from fission yeast responsible for blocking mitosis in S phase. Nature 374, 817–819 (1995). https://doi.org/10.1038/374817a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/374817a0
This article is cited by
-
An updated view on the centrosome as a cell cycle regulator
Cell Division (2022)
-
AMPKα-like proteins as LKB1 downstream targets in cell physiology and cancer
Journal of Molecular Medicine (2021)
-
Hyphal differentiation induced via a DNA damage checkpoint-dependent pathway engaged in crosstalk with nutrient stress signaling in Schizosaccharomyces japonicus
Current Genetics (2012)
-
Global dissociation of HuR-mRNA complexes promotes cell survival after ionizing radiation
The EMBO Journal (2011)
-
Yox1 links MBF‐dependent transcription to completion of DNA synthesis
EMBO reports (2011)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
