Abstract
Glycogen synthase kinase 3 (GSK-3) is homologous to the product of the Drosophila gene shaggy (zeste-white 3), which is required for signalling by wingless during Drosophila development. To test whether GSK-3 is also involved in vertebrate pattern formation, its role was investigated during early Xenopus development. It was found that dominant-negative GSK-3 mutants induced dorsal differentiation, whereas wild-type GSK-3 induced ventralization. These results indicate that GSK-3 is required for ventral differentiation, and suggest that dorsal differentiation may involve the suppression of GSK-3 activity by a wingless/wnt-related signal.
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References
Dawid, I. B. J. biol. Chem. 269, 6259–6262 (1994).
Kessler, D. S. & Melton, D. A. Science 266, 596–604 (1994).
Gerhart, J. C. et al. Development (suppl.) 107, 37–51 (1989).
Amaya, E., Musci, T. J. & Kirschner, M. W. Cell 66, 257–270 (1991).
Hemmati-Brivanlou, A. & Melton, D. A. Nature 359, 609–614 (1992).
Thomsen, G. et al. Cell 63, 485–493 (1990).
Thomsen, G. H. & Melton, D. A. Cell 74, 433–441 (1993).
Nusse, R. & Varmus, H. A. Cell 69, 1073–1087 (1992).
McMahon, A. P. & Moon, R. T. Cell 58, 1075–1084 (1989).
Smith, W. C. & Harland, R. M. Cell 67, 753–765 (1991).
Sokol, S., Christian, J. L., Moon, R. T. & Melton, D. A. Cell 67, 741–752 (1991).
Christian, J. L. & Moon, R. T. Genes Dev. 7, 13–28 (1993).
Smith, W. C. & Harland, R. M. Cell 70, 829–840 (1992).
Sasai, Y. et al. Cell 79, 779–790 (1994).
Christian, J. L., Olson, D. J. & Moon, R. T. EMBO J. 11, 33–41 (1992).
Perrimon, N. Cell 76, 781–784 (1994).
Woodgett, J. R. Sem. Cancer Biol. 5, 269–275 (1994).
Woodgett, J. R. EMBO J. 9, 2431–2448 (1990).
Sutherland, C., Leighton, I. A. & Cohen, P. Biochem. J. 296, 15–19 (1993).
Stambolic, V. & Woodgett, J. R. Biochem. J. 303, 701–704 (1994).
Siegfried, E., Chou, T.-B. & Perrimon, N. Cell 71, 1167–1179 (1992).
Ruel, L., Bourouis, M., Heitzler, P., Pantesco, V. & Simpson, P. Nature 362, 557–560 (1993).
Han, M., Golden, A., Han, V. & Sternberg, P. W. Nature 363, 133–140 (1993).
Dumont, D. et al. Genes Dev. 8, 1897–1909 (1994).
Ogura, K. et al. Genes Dev. 8, 2389–2400 (1994).
MacNicol, A. M., Muslin, A. J. & Williams, L. T. Cell 73, 571–583 (1993).
Dale, L., Howes, G., Price, B. M. & Smith, J. C. Development 115, 573–585 (1992).
Jones, C. M., Lyons, K. M., Lapan, P. M., Wright, C. V. E. & Hogan, B. L. M. Development 115, 639–647 (1992).
Kao, K. R. & Elinson, R. P. Devl Biol. 127, 64–77 (1988).
Cho, K. W. Y., Blumberg, B., Steinbeisser, H. & De Robertis, E. M. Cell 67, 1111–1120 (1991).
Sato, S. M. & Sargent, T. D. Development 112, 747–753 (1991).
Siegfried, E., Wilder, E. L. & Perrimon, N. Nature 367, 76–79 (1994).
Noordermeer, J., Klingensmith, J., Perrimon, N. & Nusse, R. Nature 367, 80–82 (1994).
McCrea, P. D., Brieher, W. M. & Gumbiner, B. M. J. cell Biol. 123, 477–484 (1993).
Heasman, J. et al. Cell 79, 791–803 (1994).
Dale, L., Matthews, G. & Colman, A. EMBO J. 12, 4471–4480 (1993).
Whitman, M. & Melton, D. A. Nature 357, 252–254 (1992).
Campbell, G., Weaver, T. & Tomlinson, A. Cell 74, 1113–1123 (1993).
Suzuki, A. et al. Proc. natn. Acad. Sci. U.S.A. 91, 10255–10259 (1994).
Graff, J. M., Thies, R. S., Song, J. J., Celeste, A. J. & Melton, D. A. Cell 79, 169–179 (1994).
Bohn, H. J. Embryol. exp. Morph. 28, 185–208 (1992).
Struhl, G. & Basler, K. Cell 72, 527–540 (1993).
Diaz-Benjumea, F. J. & Cohen, S. M. Development 120, 1661–1670 (1994).
Harwood, A. J., Plyte, S. E., Woodgett, J., Strutt, H. & Kay, R. R. Cell 80, 139–148 (1995).
Krieg, P. A. & Melton, D. A. Nucleic Acids Res. 12, 7057–7070 (1984).
Kunkel, T. A. Proc. natn. Acad. Sci. U.S.A. 82, 488–492 (1985).
Nieuwkoop, P. D. & Faber, J. Normal table of Xenopus laevis (Daudin) (North-Holland, Amsterdam, 1967).
Nishimatsu, S., Suzuki, A., Shoda, A., Murakami, K. & Ueno, N. Biochem. biophys. Res. Commun. 186, 1487–1495 (1992).
Harland, R. M. Meth. Cell Biol. 36, 685–695 (1991).
Turner, D. L. & Weintraub, H. Genes Dev. 8, 1434–1447 (1994).
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He, X., Saint-Jeannet, JP., Woodgett, J. et al. Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos. Nature 374, 617–622 (1995). https://doi.org/10.1038/374617a0
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DOI: https://doi.org/10.1038/374617a0
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