Abstract
THE RuvA and RuvB proteins of Escherichia coli, which are induced in response to DNA damage, are important in the formation of heteroduplex DNA during genetic recombination and related recombinational repair processes1. In vitro studies show that RuvA binds Holliday junctions2–5 and acts as a specificity factor that targets the RuvB ATPase, a hexameric ring protein6–7, to the junction. Together, RuvA and RuvB promote branch migration, an ATP-dependent reaction that increases the length of the heteroduplex DNA3,8–10. Electron microscopic visualization of RuvAB now provides a new insight into the mechanism of this process. We observe the formation of a tripartite protein complex in which RuvA binds the crossover and is sandwiched between two hexameric rings of RuvB. The Holliday junction within this complex adopts a square-planar structure. We propose a molecular model for branch migration, a unique feature of which is the role played by the two oppositely oriented RuvB ring motors.
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Parsons, C., Stasiak, A., Bennett, R. et al. Structure of a multisubunit complex that promotes DNA branch migration. Nature 374, 375–378 (1995). https://doi.org/10.1038/374375a0
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DOI: https://doi.org/10.1038/374375a0
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