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Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding

Abstract

DYNAMIN, a 100K member of the GTPase superfamily1, is the mammalian homologue of the Drosophila shibire gene product2,3. Mutations in shibire cause a defect in endocytosis leading to accumulation of coated pits and deep imaginations at the plasma membrane of all tissues examined4,5. Similarly, invaginated coated pits accumulate in mammalian cells overexpressing dominant-negative mutants of dynamin, establishing that dynamin is required for the formation of 'constricted' coated pits and for coated vesicle budding6. Whether dynamin functions in the classic GTPase mode as a molecular switch to regulate events leading to coated vesicle budding or instead actively participates as a mechanochemical enzyme driving coated vesicle formation is unclear7. Here we show that dynamin spontaneously self-assembles into rings and stacks of interconnected rings, comparable in dimension to the 'collars' observed at the necks of invaginated coated pits that accumulate at synaptic terminals in shibire flies4. We propose that invaginated coated pits become constricted by the assembly of dynamin into rings around their necks. A concerted conformational change would then close the rings and pinch off the budding coated vesicles.

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References

  1. Obar, R. A., Collins, C. A., Hammarback, J. A., Shpetner, H. S. & Vallee, R. B. Nature 347, 256–261 (1990).

    Article  ADS  CAS  Google Scholar 

  2. van der Bliek, A. M. & Meyerowitz, E. M. Nature 351, 411–414 (1991).

    Article  ADS  CAS  Google Scholar 

  3. Chen, M. S. et al. Nature 351, 583–586 (1991).

    Article  ADS  CAS  Google Scholar 

  4. Kosaka, T. & Ikeda, K. J. Neurobiol. 14, 207–225 (1983).

    Article  CAS  Google Scholar 

  5. Tsuruhara, T., Koenig, J. H. & Ikeda, K. Cell Tissue Res. 259, 199–207 (1990).

    Article  CAS  Google Scholar 

  6. Damke, H., Baba, T., Warnock, D. E. & Schmid, S. L. J. Cell Biol. 127, 915–934 (1994).

    Article  CAS  Google Scholar 

  7. Vallee, R. B. J. Muscle Res. Cell Motil. 13, 493–496 (1992).

    Article  CAS  Google Scholar 

  8. Herskovits, J. S., Shpetner, H. S., Burgess, C. C. & Vallee, R. B. Proc. natn. Acad. Sci. U.S.A. 90, 11468–11472 (1993).

    Article  ADS  CAS  Google Scholar 

  9. Shpetner, H. S. & Vallee, R. B. Cell 59, 421–432 (1989).

    Article  CAS  Google Scholar 

  10. Maeda, K., Nakata, T., Noda, Y., Sato-Yoshitake, R. & Hirokawa, N. Molec. Biol. Cell 3, 1181–1194 (1992).

    Article  CAS  Google Scholar 

  11. van der Bliek, A. M. et al. J. Cell Biol. 122, 553–563 (1993).

    Article  CAS  Google Scholar 

  12. Nakayama, M. et al. J. biol. Chem. 266, 15033–15038 (1993).

    Google Scholar 

  13. Musacchio, A., Gibson, T., Rice, P., Thompson, J. & Saraste, M. Trends biochem. Sci. 18, 343–348 (1993).

    Article  CAS  Google Scholar 

  14. Carter, L. L., Redelmeier, T. E., Woollenweber, L. A. & Schmid, S. L. J. Cell Biol. 120, 37–45 (1993).

    Article  CAS  Google Scholar 

  15. Schmid, S. L. Trends Cell Biol. 3, 145–148 (1993).

    Article  CAS  Google Scholar 

  16. Warnock, D. E., Terlecky, L. J. & Schmid, S. L. EMBO J. (in the press).

  17. Takei, K., McPherson, P. S., Schmid, S. L. & DeCamilli, P. Nature 374, 186–190 (1995).

    Article  ADS  CAS  Google Scholar 

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Hinshaw, J., Schmid, S. Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding. Nature 374, 190–192 (1995). https://doi.org/10.1038/374190a0

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