Abstract
ALTHOUGH eukaryotic genes are usually transcribed individually, at least a few Caenorhabditis elegans genes appear to be transcribed polycistronically in clusters resembling bacterial operons1. The spliced leader SL2 (ref. 2) is specific for trans-splicing to downstream genes in these operons1. In addition, many C. elegans pre-mRNAs are trans-spliced to SL1 (ref. 3) near the 5′ ends of pre-mRNAs4,5. Because operons have not previously been found in higher eukaryotes, we have investigated how wide-spread they are in the C. elegans genome. We identified gene clusters using the extensive data generated by the genome project6,7 and tested seven for trans-splicing specificity. All were found to fit expectations for polycistronic transcription. In addition, we sur-veyed reported C. elegans genes for trans-splicing specificity. Both methods indicate that the pre-mRNAs of about 70% of C. elegans genes are trans-spliced and as many as a quarter are transcribed in operons.
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References
Spieth, J., Brooke, G., Kuerston, S., Lea, K. & Blumenthal, T. Cell 73, 521–532 (1993).
Huang, X-Y. & Hirsh, D. Proc. natn. Acad. Sci. U.S.A. 86, 8640–8644 (1989).
Krause, M. & Hirsh, D. Cell 49, 753–761 (1987).
Conrad, R., Thomas, J., Spieth, J. & Blumenthal, T. Molec. cell. Biol. 11, 1921–1926 (1991).
Conrad, R., Liou, R. F. & Blumenthal, T. EMBO J. 12, 1249–1255 (1993).
Sulston, J. et al. Nature 356, 37–41 (1992).
Wilson, R. et al. Nature 368, 32–38 (1994).
Huang, L. S., Tzou, P. & Sternberg, P. W. Molec. Biol. Cell 5, 395–412 (1994).
Clark, S. G., Lu, X. & Horvitz, H. R. Genetics 137, 987–997 (1994).
Lee, Y. H., Huang, X-Y., Hirsh, D., Fox, G. E. & Hecht, R. M. Gene 121, 227–235 (1992).
Waterston, R. et al. Nature Genet. 1, 114–123 (1992).
Huang, X.-Y. et al. J. molec. biol. 206, 411–424 (1989).
Land, M., Islas-Trejo, A. & Rubin, C. S. J. biol. Chem. 259, 14820–14827 (1994).
Kuwabara, P. E., Okkema, P. G. & Kimble, J. Molec. Biol. Cell 3, 461–473 (1992).
Hu, E. & Rubin, C. S. J. biol. Chem. 266, 19796–19802 (1991).
Capowski, E. E., Martin, P., Garvin, C. & Strome, S. Genetics 129, 1061–1072 (1991).
Morgan, W. M. & Greenwald, I. Molec. cell. Biol. 13, 7133–7143 (1993).
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Zorio, D., Cheng, N., Blumenthal, T. et al. Operons as a common form of chromosomal organization in C. elegans. Nature 372, 270–272 (1994). https://doi.org/10.1038/372270a0
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DOI: https://doi.org/10.1038/372270a0
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