Abstract
Blood cell development relies on the expansion and maintenance of haematopoietic stem and progenitor cells in the embryo. By gene targeting in mouse embryonic stem cells, we demonstrate that the transcription factor GATA-2 plays a critical role in haematopoiesis, particularly of an adult type. We propose that GATA-2 regulates genes controlling growth factor responsiveness or the proliferative capacity of early haematopoietic cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Moore, M. S. A. & Metcalf, D. Br. J. Haemat. 18, 279–296 (1970).
Medvinsky, A. L., Samoylina, N. L., Muller, A. M. & Dzierzak, E. A. Nature 364, 64–66 (1993).
Godin, I. E., Garcia-Porrero, J. A., Coutinho, A., Dieterlen-Lievre, F. & Marcos, M. A. R. Nature 364, 67–70 (1993).
Orkin, S. H. Blood 80, 575–581 (1992).
Tsai, S. F. et al. Nature 339, 446–451 (1989).
Evans, T. & Felsenfeld, G. Cell 58, 877–885 (1989).
Pevny, L. et al. Nature 349, 257–260 (1991).
Simon, M. C. et al. Nature Genet. 1, 92–98 (1992).
Weiss, M. J., Keller, G. & Orkin, S. H. Genes Dev. 8, 1184–1197 (1994).
Yamamoto, M. et al. Genes Dev. 4, 1650–1662 (1990).
Dorfman, D. M., Wilson, D. B., Bruns, G. A. & Orkin, S. H. J. biol. Chem. 267, 1279–1285 (1992).
Visvader, J. & Adams, J. M. Blood 82, 1493–1501 (1993).
Leonard, M., Brice, M., Engel, J. D. & Papayannopoulou, T. Blood 82, 1071–1079 (1993).
Mouthon, M.-A. et al. Blood 81, 647–655 (1993).
Briegel, K. et al. Genes Dev. 7, 1097–1109 (1993).
Kelley, C., Yee, K., DeCaprio, J., Harland, R. M. & Zon, L. I. Devl Biol. (in the press).
Martin, D. & Orkin, S. Genes Dev. 4, 1886–1898 (1990).
Robertson, E. J., Bradley, A., Kuehn, M. & Evans, M. Nature 323, 445–448 (1986).
Li, E., Bestor, T. H. & Jaenisch, R. Cell 69, 915–926 (1992).
Mortensen, R. M., Conner, D. A., Chao, S., Geisterfer-Lowrance, A. A. T. & Seidman, J. G. Molec. cell. Biol. 12, 2391–2395 (1992).
Chen, J., Lansford, R., Stewart, V., Young, F. & Alt, F. W. Proc. natn. Acad. Sci. U.S.A. 90, 4528–4532 (1993).
Wiles, M. V. & Keller, G. Development 111, 259–267 (1991).
Keller, G., Kennedy, M., Papayannopoulou, T. & Wiles, M. V. Molec. cell. Biol. 13, 472–486 (1993).
Tsai, S.-F., Strauss, E. & Orkin, S. H. Genes Dev. 5, 919–931 (1991).
Russell, E. S. Adv. Genet. 20, 358–420 (1979).
Mucenski, M. L. et al. Cell 65, 677–689 (1991).
Lee, E. Y.-H. L. et al. Nature 359, 288–294 (1992).
Jacks, T. et al. Nature 359, 295–300 (1992).
Clarke, A. R. et al. Nature 359, 328–330 (1992).
Chen, J. et al. Curr. Biol. 3, 405–413 (1993).
Fleischman, R. A. Trends Genet. 9, 285–290 (1993).
Geissler, E. N., McFarland, E. C. & Russell, E. S. Genetics 97, 337–361 (1981).
Russell, E. S., Thompson, M. W. & McFarland, E. C. Genetics 58, 259–270 (1968).
Wong, P. M. C. et al. Proc. natn. Acad. Sci. U.S.A. 83, 3851–3854 (1986).
Tybulewicz, V. L. J., Crawford, C. E., Jackson, P. K., Bronson, R. T. & Mulligan, R. C. Cell 65, 1153–1163 (1991).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning, a Laboratory Manual 2nd edn, (Cold Spring Harbor Laboratory Press, New York, 1989).
Robertson, E. Teratocarcinomas and Embryonic Stem Cells: A Practical Approach (IRL Press, Oxford, 1987).
Whitney, J. B. Biochem. Genet. 16, 667–672 (1978).
Karasuyma, H. & Melchers, F. Eur. J. Immun. 18, 97–104 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tsai, FY., Keller, G., Kuo, F. et al. An early haematopoietic defect in mice lacking the transcription factor GATA-2. Nature 371, 221–226 (1994). https://doi.org/10.1038/371221a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/371221a0
This article is cited by
-
Integrative analysis of the ST6GALNAC family identifies GATA2-upregulated ST6GALNAC5 as an adverse prognostic biomarker promoting prostate cancer cell invasion
Cancer Cell International (2023)
-
In vivo clonal tracking reveals evidence of haemangioblast and haematomesoblast contribution to yolk sac haematopoiesis
Nature Communications (2023)
-
GATA2 Deficiency: Predisposition to Myeloid Malignancy and Hematopoietic Cell Transplantation
Current Hematologic Malignancy Reports (2023)
-
Role of the pioneer transcription factor GATA2 in health and disease
Journal of Molecular Medicine (2023)
-
Noncoding variants alter GATA2 expression in rhombomere 4 motor neurons and cause dominant hereditary congenital facial paresis
Nature Genetics (2023)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.