Abstract
By adapting a commercially available, general purpose laboratory robot, it is possible to produce high-density gridded hybridization filters of clone colonies or DNA products. We are using this system to produce 60–90,8 x 12 cm filters in an 8-hour day, each containing 3,456 clones arranged in 96, 6 x 6 grids.
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References
Carrano, A. V. et al. Genomics 4, 129–136 (1989).
Olsen, A. S. et al. BioTechniques 14, 116–122 (1993).
Carrano, A. V. et al. Genome 31, 1059–1065 (1989).
Lennon, G. G. & Lehrach, H. TIGS 7, 314–317 (1991).
Schoeny, D. E. & Rollheiser, J. J. Am. Lab. 42–47 (1991).
Lennon, G. in High Density Grid Technologies, Automated DNA Sequencing and Analysis (ed. Adams, M.) 126–130 (Academic, London, 1994).
Shizuka, H. et al. Proc. natn. Acad. Sci. U.S.A. 89, 8794–8797 (1992).
Ioannou, P. et al. Nature Genet. 6, 84–89 (1994).
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Copeland, A., Lennon, G. Rapid arrayed filter production using the 'ORCA' robot. Nature 369, 421–422 (1994). https://doi.org/10.1038/369421a0
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DOI: https://doi.org/10.1038/369421a0
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