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Evidence that the S6 segment of the Shaker voltage-gated K+ channel comprises part of the pore

Nature volume 367, pages 179–182 (1994)Cite this article

Abstract

POTASSIUM channels are highly selective and allow the rapid flux of potassium ions through their pore1. Several studies have implicated the H5 (P or SS1-SS2) segment2,3 as part of the pore in voltage-gated ion channels4–10. The proposal that H5 spans at least 80% of the electric potential drop across the K+ channel pore5,11 is based on altered internal tetraethylammonium sensitivity arising from mutations of H5 residues that are 100% conserved among K+ channels having differing sensitivity to tetraethyl-ammonium5–7,12,13. Here we report that the S6 segment is also involved in K+ ion permeation and in governing the sensitivity to internal tetraethylammonium and barium. Transplanting the S6 segment of NGK2 into Shaker causes this S6 chimaera to adopt the single-channel conductance and sensitivity to internal tetraethylammonium and barium ions from the NGK2 channel. The differences between NGK2 and Shaker in external tetraethylammonium sensitivity, but not single-channel conductance, can be attributed to the differences in their H5 sequences. Three non-conserved S6 residues have been found to affect either single-channel conductance or internal tetraethylammonium sensitivity.

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References

  1. Hille, B. Ionic Channels of Excitable Membranes (Sinauer, Sunderland, Massachusetts, 1991).

    Google Scholar 

  2. Guy, H. R. & Seetharamulu, P. Proc. natn. Acad. Sci. U.S.A. 83, 508–521 (1986).

    Article  ADS  CAS  Google Scholar 

  3. Tempel, B. L., Papazian, D. M., Schwarz, T. L., Jan, Y. N. & Jan, L. Y. Science 237, 770–775 (1987).

    Article  ADS  CAS  Google Scholar 

  4. Yool, A. J. & Schwarz, T. S. Nature 349, 700–704 (1991).

    Article  ADS  CAS  Google Scholar 

  5. Yellen, G., Jurman, M., Abramson, T. & MacKinnon, R. Science 251, 939–942 (1991).

    Article  ADS  CAS  Google Scholar 

  6. Hartmann, H. A. et al. Science 251, 942–944 (1991).

    Article  ADS  CAS  Google Scholar 

  7. Kirsch, G. E. et al. Neuron 8, 499–505 (1992).

    Article  CAS  Google Scholar 

  8. Heinemann, S. H., Terlau, H., Stühmer, W., Imoto, K. & Numa, S. Nature 356, 441–443 (1992).

    Article  ADS  CAS  Google Scholar 

  9. Backx, P. H., Yue, D. T., Lawrence, J. H., Marban, E. & Tomaselli, G. F. Science 257, 248–251 (1992).

    Article  ADS  CAS  Google Scholar 

  10. Heginbotham, L., Abramson, T. & MacKinnon, R. Science 258, 1152–1155 (1992).

    Article  ADS  CAS  Google Scholar 

  11. Miller, C. Science 252, 1092–1096 (1991).

    Article  ADS  CAS  Google Scholar 

  12. Kirsch, G. E., Taglialatela, M. &. & Brown, A. M. Am. J. Physiol. 261, C583–590 (1991).

    Article  CAS  Google Scholar 

  13. Taglialatela, M. et al. Molec. Pharmac. 40, 299–307 (1991).

    CAS  Google Scholar 

  14. Schwarz, T. S., Tempel, B. L., Papazian, D. M., Jan, Y. N. & Jan, L. Y. Nature 331, 137–142 (1988).

    Article  ADS  CAS  Google Scholar 

  15. Yokoyama, S. et al. FEBS Lett. 259, 37–42 (1989).

    Article  CAS  Google Scholar 

  16. Hoshi, T., Zagotta, W. N. & Aldrich, R. W. Science 250, 533–538 (1990).

    Article  ADS  CAS  Google Scholar 

  17. Armstrong, C. M. & Binstock, L. J. gen. Physiol. 48, 859–872 (1965).

    Article  CAS  Google Scholar 

  18. Armstrong, C. M. J. gen. Physiol. 50, 491–503 (1966).

    Article  CAS  Google Scholar 

  19. Armstrong, C. M. J. gen. Physiol. 54, 553–575 (1969).

    Article  CAS  Google Scholar 

  20. Armstrong, C. M. J. gen. Physiol. 58, 413–437 (1971).

    Article  CAS  Google Scholar 

  21. Armstrong, C. M. & Hille, B. J. gen. Physiol. 59, 388–400 (1972).

    Article  CAS  Google Scholar 

  22. Armstrong, C. M. & Taylor, S. R. Biophys. J. 30, 473–488 (1980).

    Article  CAS  Google Scholar 

  23. Armstrong, C. M., Swenson, R. P. & Taylor, S. R. J. gen. Physiol. 80, 663–682 (1982).

    Article  CAS  Google Scholar 

  24. Hoshi, T., Zagotta, W. N. & Aldrich, R. W. Neuron 7, 547–556 (1991).

    Article  CAS  Google Scholar 

  25. Choi, K. L., Mossman, C., Aube, J. & Yellen, G. Neuron 10, 533–541 (1993).

    Article  CAS  Google Scholar 

  26. Isacoff, E. Y., Jan, Y. N. & Jan, L. Y. Nature 353, 86–90 (1991).

    Article  ADS  CAS  Google Scholar 

  27. Slesinger, P. A., Jan, Y. N. & Jan, L. Y. Neuron 11, 739–749 (1993).

    Article  CAS  Google Scholar 

  28. Woodhull, A. M. J. gen. Physiol. 61, 687–708 (1973).

    Article  CAS  Google Scholar 

  29. Baldwin, T. J., Tsaur, M-L., Lopez, G. A., Jan, Y. N. & Jan, L. Y. Neuron 7, 471–483 (1991).

    Article  CAS  Google Scholar 

  30. Lopez, G. A., Jan, Y. N. & Jan, L. Y. Neuron 7, 327–336 (1991).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Howard Hughes Medical Institute and Departments of Physiology and Biochemistry, University of California at San Francisco, Parnassus and Third Avenues, U-426, San Francisco, California, 94143-0724, USA

    George A. Lopez, Yuh Nung Jan & Lily Y. Jan

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  1. George A. Lopez
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  2. Yuh Nung Jan
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Lopez, G., Nung Jan, Y. & Jan, L. Evidence that the S6 segment of the Shaker voltage-gated K+ channel comprises part of the pore. Nature 367, 179–182 (1994). https://doi.org/10.1038/367179a0

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