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Electrical signalling and systemic proteinase inhibitor induction in the wounded plant

Nature volume 360, pages 62–65 (1992)Cite this article

Abstract

THE wound response of several plant species involves the activation of proteinase inhibitor (pin) genes and the accumulation of pin proteins at the local site of injury and systemically throughout the unwounded aerial regions of the plant1,2. It has been suggested that a mobile chemical signal is the causal agent linking the local wound stimulus to the distant systemic response, and candidates such as oligosaccharides3, abscisic acid4 and a polypeptide5,6 have been put forward. But the speed of transmission is high for the transport of a chemical signal in the phloem. The wound response of tomato plants can be inhibited by salicylic acid7 and agents like fusicoccin that affect ion transport8, and wounding by heat9 or physical injury produces electrical activity that has similarities to the epithelial conduction system10 used to transmit a stimulus in the defence responses of some lower animals11. Here we design experiments to distinguish between a phloem-transmissible chemical signal and a physically propagated signal based on electrical activity. We show that translocation in the phloem of tomato seedlings can be completely inhibited without effect on the systemic accumulation of pin transcripts and pin activity, and without hindrance to propagated electrical signals.

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References

  1. Ryan, C. A. A Rev. Phytopathol. 28, 425–449 (1990).

    Article  CAS  Google Scholar 

  2. Bowles, D. J. A. Rev. Biochem. 59, 873–907 (1990).

    Article  CAS  Google Scholar 

  3. Ryan, C. A. & Farmer, E. E. A. Rev. Pl. Physiol. Pl. molec. Biol. 42, 651–674 (1991).

    Article  CAS  Google Scholar 

  4. Pena-cortes, H., Willmitzer, L. & Sanchez-Serrano, J. Pl. Cell 3, 963–972 (1991).

    Article  CAS  Google Scholar 

  5. Pearce, G., Strydom, D., Johnson, S. & Ryan, C. A. Science 253, 895–898 (1991).

    Article  ADS  CAS  Google Scholar 

  6. Farmer, E. E. & Ryan, C. A. Pl. Cell 4, 129–134 (1992).

    Article  CAS  Google Scholar 

  7. Doherty, H. M., Selvendran, R. A. & Bowles, D. J. Physiol. molec. Pl. Path. 33, 377–384 (1988).

    Article  CAS  Google Scholar 

  8. Doherty, H. M. & Bowles, D. J. Pl. Cell Envir. 13, 851–855 (1990).

    Article  CAS  Google Scholar 

  9. Wildon, D. C., Doherty, H. M., Eagles, G. Bowles, D. J. & Thain, J. F. Ann. Bot. 64, 691–695 (1989).

    Article  Google Scholar 

  10. Mackie, G. O. Am. Zool. 5, 439–453 (1965).

    Article  CAS  Google Scholar 

  11. Anderson, P. A. V. Prog. Neurobiol. 15, 161–203 (1980).

    Article  CAS  Google Scholar 

  12. Gasser, H. S. Am. J. Physiol. 97, 254–270 (1931).

    CAS  Google Scholar 

  13. Blatt, F. J. Biochim. biophys. Acta 339, 382–389 (1974).

    Article  CAS  Google Scholar 

  14. Lang, A. & Minchin, P. E. H. J. exp. Bot. 37, 389–398 (1986).

    Article  Google Scholar 

  15. Baker, D. A. & Milburn, J. A. (eds) Transport of Photoassimilates (Longman, Harlow, 1989).

  16. Pena-Cortes, H., Sanchez-Serrano, J., Rocha-Sosa, M. & Willmitzer, L. Planta 174, 84–89 (1988).

    Article  CAS  Google Scholar 

  17. Farmer, E. E., Johnson, R. R. & Ryan, C. A. Pl. Physiol. 98, 995–1002 (1992).

    Article  CAS  Google Scholar 

  18. Nelson, C. E., Walker-Simmons, M., Makus, D., Zuroske, G., Graham, J. & Ryan, C. A. in Mechanisms of Plant Resistance to Insects (ed. Hedin, P.) 103–122 (American Chemical Society, Washington DC, 1983).

    Book  Google Scholar 

  19. Tyree, M. T. J. theor. Biol. 26, 181–214 (1970).

    Article  CAS  Google Scholar 

  20. Malone, M. & Stankovic, B. Pl. Cell Envir. 14, 431–436 (1991).

    Article  Google Scholar 

  21. Malone, M. Planta 187, 505–510 (1992).

    Article  CAS  Google Scholar 

  22. Robards, A. W. & Lucas, W. J. A. Rev. Pl. Physiol. Pl. molec. Biol. 41, 369–419 (1990).

    Article  Google Scholar 

  23. Robards. A. W., Lucas, W. J., Pitts, J. D., Jongsma, H. J. & Spray, D. C. (eds) Parallels in Cell to Cell Junctions in Plants and Animals (Springer, Berlin, 1990).

    Google Scholar 

  24. Pickard, B. Bot. Rev. 39, 172–201 (1973).

    Article  Google Scholar 

  25. Pickard, B. Naturwissenschaften 61, 60–64 (1974).

    Article  ADS  Google Scholar 

  26. Davies, E. Pl. Cell Envir. 10, 623–631 (1987).

    Article  Google Scholar 

  27. Smallwood, M., Gurr, S. J., McPherson, M. J. & Bowles, D. J. Biochem. J. 281, 501–505 (1992).

    Article  CAS  Google Scholar 

  28. Longmann, J., Schell, J. & Willmitzer, L. Analyt. Biochem. 163, 16–20 (1987).

    Article  Google Scholar 

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Authors and Affiliations

  1. School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK

    D. C. Wildon, J. F. Thain, I. R. Gubb, A. J. Reilly & Y. D. Skipper

  2. The Horticulture and Food Research Institute of New Zealand Ltd, Lower Hutt, New Zealand

    P. E. H. Minchin

  3. Centre for Plant Biochemistry and Biotechnology, University of Leeds, Leeds, LS2 9JT, UK

    H. M. Doherty, P. J. O'Donnell & D. J. Bowles

Authors
  1. D. C. Wildon
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  2. J. F. Thain
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  3. P. E. H. Minchin
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  4. I. R. Gubb
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  5. A. J. Reilly
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  6. Y. D. Skipper
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  7. H. M. Doherty
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  8. P. J. O'Donnell
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  9. D. J. Bowles
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Wildon, D., Thain, J., Minchin, P. et al. Electrical signalling and systemic proteinase inhibitor induction in the wounded plant. Nature 360, 62–65 (1992). https://doi.org/10.1038/360062a0

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