Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Requirement of phosphatidylinositol 4,5-bisphosphate for α-actinin function

Nature volume 359, pages 150–152 (1992)Cite this article

Abstract

INOSITOL phospholipid turnover is enhanced during mitogenic stimulation of cells by growth factors1 and the breakdown of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) may be important in triggering cell proliferation. PtdInsP2 also binds actin-binding proteins to regulate their activity2–7, but it is not yet understood how this control is achieved. The protein α-actinin from striated muscle contains large amounts of endogenous PtdInsP2, whereas that from smooth muscle has only a little but will bind exogenously added PtdInsP2. In vitro α-actinin binds to F-actin and will crosslink actin filaments, increasing the viscosity of F-actin solutions8,9. We report here that α-actinin from striated muscle is an endogenous PtdInsP2-bound protein and that the specific interaction between α-actinin and PtdInsP2 regulates the F-actin-gelating activity of α-actinin. Although the F-actin-gelating activity of α-actinin from smooth muscle is much reduced compared with that from striated muscle, exogenous PtdInsP2 can enhance the activity of smooth muscle α-actinin to the level seen in striated muscles. These results show that PtdInsP2 is present in striated muscle α-actinin and that it is necessary for α-actinin to realize its maximum gelating activity.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Rozengurt, E. Science 234, 161–166 (1986).

    Article  ADS  CAS  Google Scholar 

  2. Lassing, I. & Lindberg, U. Nature 314, 472–474 (1985).

    Article  ADS  CAS  Google Scholar 

  3. Janmey, P. A. & Stossel, T. P. Nature 325, 362–364 (1987).

    Article  ADS  CAS  Google Scholar 

  4. Janmey, P. A., Iida, K., Yin, H. L. & Stossel, T. P. J. biol. Chem. 262, 12228–12236 (1987).

    CAS  PubMed  Google Scholar 

  5. Yu, F., Johnston, P. A., Sudhof, T. C. & Yin, H. L. Science 250, 1413–1415 (1990).

    Article  ADS  CAS  Google Scholar 

  6. Heiss, S. H. & Cooper, J. A. Biochemistry 30, 8753–8758 (1991).

    Article  CAS  Google Scholar 

  7. Anderson, R. A. & Marchesi, V. T. Nature 318, 295–298 (1985).

    Article  ADS  CAS  Google Scholar 

  8. Ebashi, S., Ebashi, F. & Maruyama, K. Nature 203, 645–646 (1964).

    Article  ADS  CAS  Google Scholar 

  9. Maruyama, K. & Ebashi, S. J. Biochem. 58, 7–12 (1965).

    Article  Google Scholar 

  10. Matuoka, K., Fukami, K., Nakanishi, O., Kawai, S. & Takenawa, T. Science 239, 640–643 (1988).

    Article  ADS  CAS  Google Scholar 

  11. Masaki, T., Endo, M. & Ebashi, S. J. Biochem. 62, 630–632 (1967).

    Article  CAS  Google Scholar 

  12. Pollard, T. D. & Cooper, J. A. Meth. Enzym. 85, 211–233 (1982).

    Article  CAS  Google Scholar 

  13. Bennett, J. P., Zaner, K. S. & Stossel, T. P. Biochemistry 23, 5081–5086 (1984).

    Article  CAS  Google Scholar 

  14. Landon, F., Gache, Y., Touitou, H. & Olomucki, A. Eur. J. Biochem. 153, 231–237 (1985).

    Article  CAS  Google Scholar 

  15. Furuhashi, K., Inagaki, M., Hatano, S., Fukami, K. & Takenawa, T. Biochem. biophys. Res. Commun. 184, 121–1265 (1992).

    Article  Google Scholar 

  16. Meyer, R. K., Schindler, H. & Burger, M. M. Proc. natn. Acad. Sci. U.S.A. 79, 4280–4284 (1982).

    Article  ADS  CAS  Google Scholar 

  17. Burn, P., Rotman, A., Meyer, R. K. & Burger, M. M. Nature 314, 469–472 (1985).

    Article  ADS  CAS  Google Scholar 

  18. Endo, T. & Masaki, T. J. Biochem. 92, 1457–1468 (1982).

    Article  CAS  Google Scholar 

  19. Kobayashi, R., Itoh, H. & Tashima, Y. Eur. J. Biochem. 143, 125–131 (1984).

    Article  CAS  Google Scholar 

  20. Endo, T. & Masaki, T. Biochem. biophys. Res. Commun. 107, 1467–1474 (1982).

    Article  CAS  Google Scholar 

  21. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  22. Khyse-Anderson, J. J. biochem. biophys. Meth. 10, 203–209 (1984).

    Article  Google Scholar 

  23. Feramisco, J. R. & Burridge, K. J. biol. Chem. 255, 1194–1199 (1980).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology, Sakae-cho, Itabashi-ku, Tokyo, 173, Japan

    Kiyoko Fukami & Tadaomi Takenawa

  2. Laboratory of Experimental Radiology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, Aichi, 464, Japan

    Kiyoshi Furuhashi & Masaki Inagaki

  3. Department of Biology, Chiba University, Yayoi-cho, Chiba, 260, Japan

    Takeshi Endo

  4. Department of Molecular Biology, School of Science, Nagoya University, Chikusa-ku, Nagoya, Aichi, 464, Japan

    Sadashi Hatano

Authors
  1. Kiyoko Fukami
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kiyoshi Furuhashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masaki Inagaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takeshi Endo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sadashi Hatano
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tadaomi Takenawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fukami, K., Furuhashi, K., Inagaki, M. et al. Requirement of phosphatidylinositol 4,5-bisphosphate for α-actinin function. Nature 359, 150–152 (1992). https://doi.org/10.1038/359150a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/359150a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing