Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Phosphorylation of transcription factor p62TCF by MAP kinase stimulates ternary complex formation at c-fos promoter

Nature volume 358, pages 414–417 (1992)Cite this article

Abstract

TRANSCRIPTION of the proto-oncogene c-fos is stimulated rapidly and transiently by serum growth factors and mitogens1. Critical for this response is the serum-response element which is bound in vivo in a ternary complex containing the transcription factors p67SRF and p62TCF (ref. 2). Disruption of the ternary complex correlates with impaired induction by serum and phorbol ester3,4. Mitogen-activated protein (MAP) kinase is a serine/ threonine kinase which is activated 1-5 minutes after treatment of cells with mitogens and growth factors5–8 that induce re-entry into the cell cycle, making MAP kinase a candidate for the transmission of proliferative signals. Here we show that p62TCF is phosphorylated by MAP kinase in vitro and that phosphorylation results in enhanced ternary complex formation. Serum-starved Swiss 3T3 cells treated with epidermal growth factor, which induces MAP kinase in these cells9, are induced to express c-fos and yield p62TCF active in ternary complex formation. In contrast, treatment of Swiss 3T3 cells with insulin, which does not activate MAP kinase under these conditions9, does not lead to enhanced ternary complex formation nor does it induce c-fos transcription. Our results link the expression of the human c-fos proto-oncogene to signal transduction pathways known to be activated before its own induction.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Herschman, H. R. A. Rev. Biochem. 60, 281–319 (1991).

    Article  CAS  Google Scholar 

  2. Herrera, R. E., Shaw, P. E. & Nordheim, A. Nature 340, 68–70 (1989).

    Article  ADS  CAS  Google Scholar 

  3. Shaw, P. E., Schröter, H. & Nordheim, A. Cell 56, 563–572 (1989).

    Article  CAS  Google Scholar 

  4. Graham, R. & Gilman, M. Science 251, 189–192 (1991).

    Article  ADS  CAS  Google Scholar 

  5. Ray, L. B. & Sturgill, T. W. Proc. natn. Acad. Sci. U.S.A. 84, 1502–1506 (1987).

    Article  ADS  CAS  Google Scholar 

  6. Hoshi, M., Nishida, E. & Sakai, H. J. biol. Chem. 263, 5396–5401 (1988).

    CAS  PubMed  Google Scholar 

  7. Boulton, T. G. et al. Cell 65, 663–675 (1991).

    Article  CAS  Google Scholar 

  8. Cobb, M. H., Boulton, T. G. & Robbins, D. J. Cell Reg. 2, 965–978 (1991).

    Article  CAS  Google Scholar 

  9. Ballou, L., Luther, H. & Thomas, G. Nature 349, 348–350 (1991).

    Article  ADS  CAS  Google Scholar 

  10. Schröter, H. et al. EMBO J. 9, 1123–1130 (1990).

    Article  Google Scholar 

  11. Susa, M. & Thomas, G. Proc. natn. Acad. Sci. U.S.A. 87, 7040–7044 (1990).

    Article  ADS  CAS  Google Scholar 

  12. Susa, M. & Thomas, G. Cell 57, 817–824 (1989).

    Article  CAS  Google Scholar 

  13. Chen, R.-H. & Blenis, J. Molec. cell. Biol. 10, 3204–3215 (1990).

    Article  CAS  Google Scholar 

  14. Hall, F. L., et al. J. biol. Chem. 266, 17430–17440 (1991).

    CAS  PubMed  Google Scholar 

  15. Buhrow, S. A., Cohen, S., Garbers, D. L. & Staros, J. V. J. biol. Chem. 258, 7824–7827 (1983).

    CAS  Google Scholar 

  16. Malik, R. K., Roe, M. W. & Blackshear, P. J. J. biol. Chem. 266, 8576–8582 (1991).

    CAS  PubMed  Google Scholar 

  17. Manak, J. R., deBisschop, N., Kris, R. M. & Prywes, R. Genes Dev. 4, 955–967 (1990).

    Article  CAS  Google Scholar 

  18. Manak, J. R. & Prywes, R. Molec. cell. Biol. 11, 3652–3659 (1991).

    Article  CAS  Google Scholar 

  19. Marais, R. M., Hsuan, J. J., McGuigan, C., Wynne, J. & Treisman, R. EMBO J. 11, 97–105 (1992).

    Article  CAS  Google Scholar 

  20. Ackermann, P. & Osheroff, N. J. biol. Chem. 264, 11958–11965 (1989).

    Google Scholar 

  21. Ackermann, P., Glover, C. V. C. & Osheroff, N. Proc. natn. Acad. Sci. U.S.A. 87, 821–825 (1990).

    Article  ADS  Google Scholar 

  22. Prywes, R., Dutta, A., Cromlish, J. A. & Roeder, R. G. Proc. natn. Acad. Sci. U.S.A. 85, 7206–7210 (1988).

    Article  ADS  CAS  Google Scholar 

  23. Chen, R.-H., Sarnecki, C. & Blenis, J. Molec. cell. Biol. 12, 915–927 (1992).

    Article  CAS  Google Scholar 

  24. Hipskind, R. A., Rao, V. N., Mueller, C. G. F., Reddy, E. S. P. & Norheim, A. Nature 354, 531–534 (1991).

    Article  ADS  CAS  Google Scholar 

  25. Dalton, S. & Treisman, R. Cell 68, 597–612 (1992).

    Article  CAS  Google Scholar 

  26. Alvarez, E. et al. J. biol. Chem. 266, 15277–15285 (1991).

    CAS  PubMed  Google Scholar 

  27. Gonzalez, F. A., Raden, D. L. & Davis, R. I. J. biol. Chem. 266, 22159–22163 (1991).

    CAS  PubMed  Google Scholar 

  28. Greenberg, M. E., Green, L. A. & Ziff, E. J. biol. Chem. 260, 14101–14110 (1985).

    CAS  PubMed  Google Scholar 

  29. Gomez, N., Tonks, N. K., Morrison, C., Harnar, T. & Cohen, P. FEBS Lett. 271, 119–122 (1991).

    Article  Google Scholar 

  30. Ferrari, S. & Thomas, G. Meth. Enzym. 200, 159–169 (1991).

    Article  CAS  Google Scholar 

  31. Ballou, L., Jenö, P. & Thomas, G. J. biol. Chem. 263, 1188–1194 (1988).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck-lnstitut für Immunbiologie, Spemann Laboratories, Postfach 1169, Stübeweg 51, W-7800, Freiburg, Germany

    Hendrik Gille, Andrew D. Sharrocks & Peter E. Shaw

Authors
  1. Hendrik Gille
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andrew D. Sharrocks
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter E. Shaw
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gille, H., Sharrocks, A. & Shaw, P. Phosphorylation of transcription factor p62TCF by MAP kinase stimulates ternary complex formation at c-fos promoter. Nature 358, 414–417 (1992). https://doi.org/10.1038/358414a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/358414a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing