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A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants

Abstract

Male fertility was restored to genetically engineered male sterile oilseed rape plants. Male sterile plants that express a chimaeric ribonuclease gene in the anther tapetal cell layer were crossed with male fertile plants that were transformed with a chimaeric tapetal-cell-specific ribonuclease-inhibitor gene. F1 progeny expressing both genes are restored to male fertility by the suppression of cytotoxic ribonuclease activity in the anther by the formation of cell-specific RNase/RNase inhibitor complexes. Genetically engineered male sterility and fertility restorer genes should facilitate hybrid seed production in crop plants.

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References

  1. Mayo, H. The Theory of Plant Breeding (Clarendon, Oxford, 1980).

    Google Scholar 

  2. Haring, V. et al. Science 250, 937–941 (1990).

    Article  ADS  CAS  Google Scholar 

  3. Levings, C. S. Science 250, 942–947 (1990).

    Article  ADS  CAS  Google Scholar 

  4. Kaul, M. L. H. Monographs on Theoretical and Applied Genetics Vol. 10 Male Sterility in Higher Plants (Springer, Berlin, 1988).

    Book  Google Scholar 

  5. Seurinck, J., Truettner, J. & Goldberg, R. B. Nucleic Acids Res, 18, 3403 (1990).

    Article  CAS  Google Scholar 

  6. Koltunow, A. M. et al. Plant Cell 2, 1201–1224 (1990).

    Article  CAS  Google Scholar 

  7. Mariani, C. et al. Nature 347, 737–741 (1990).

    Article  ADS  CAS  Google Scholar 

  8. Hartley, R. W. Trends Biochem. Sci. 14, 450–454 (1989).

    Article  CAS  Google Scholar 

  9. Hartley, R. W. J. molec. Biol. 202, 913–915 (1988).

    Article  CAS  Google Scholar 

  10. De Block, M. et al. EMBO J. 6, 2513–2518 (1987).

    Article  CAS  Google Scholar 

  11. De Block, M., De Brouwer, D. & Tenning, P. Pl. Physiol. 91, 695–701 (1989).

    Article  Google Scholar 

  12. De Block, M. & De Brouwer, D. Pl. J. 2, 261–266 (1992).

    Article  CAS  Google Scholar 

  13. Jones, D. G., Dunsmuir, P. & Bedbrook, J. EMBO J. 4, 2411–2418 (1985).

    Article  CAS  Google Scholar 

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Mariani, C., Gossele, V., Beuckeleer, M. et al. A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants. Nature 357, 384–387 (1992). https://doi.org/10.1038/357384a0

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