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The complete DNA sequence of yeast chromosome III

Nature volume 357, pages 3846 (07 May 1992) | Download Citation

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Abstract

The entire DNA sequence of chromosome III of the yeast Saccharomyces cerevisiae has been determined. This is the first complete sequence analysis of an entire chromosome from any organism. The 315-kilobase sequence reveals 182 open reading frames for proteins longer than 100 amino acids, of which 37 correspond to known genes and 29 more show some similarity to sequences in databases. Of 55 new open reading frames analysed by gene disruption, three are essential genes; of 42 non-essential genes that were tested, 14 show some discernible effect on phenotype and the remaining 28 have no overt function.

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Author information

Affiliations

  1. Manchester Biotechnology Centre, UMIST, Manchester M60 1QD, UK;

    • S. G. Oliver
    • , R. Anwar
    • , A. Brown
    • , M. E. Gent
    • , K. J. Indje
    • , C. M. James
    • , L. I. Stateva
    •  & J. R. Warmington
  2. Department of Cell Biology Genetics. Leiden University, The Netherlands;

    • Q. J. M. van der Aart
    •  & H. Y. Steensma
  3. Dipartimento di Genetica e di Biologia dei Microrganismi, Universita di Milano, Italy;

    • M. L. Agostoni-Carbone
    • , C. Carcano
    • , G Lucchini
    • , M. Muzi Falconi
    • , L. Panzeri
    •  & P. Plevani
  4. LBMS, F-33000 Bordeaux, France;

    • M. Aigle
    • , N. Biteau
    •  & M. Crouzet
  5. Dipartimento di Fisiologia e Biochimica, Universita di Milano, Italy;

    • L. Alberghina
    • , E. Martegani
    •  & F. Rodriguez
  6. lnstituto Molecular Biology and Biotechnology, PO Box 1527, GR-71110 Heraklio,Crete;

    • D. Alexandraki
    • , P. Galland
    • , G. Thireos
    •  & M. Tzermia
  7. lnstitut Curie, Centre Universitaire, F-91405 Orsay, France;

    • G. Antoine
    • , P. Benit
    • , R. Chanet
    • , F. Fabre
    • , G. Faye
    • , H. Fukuhara
    • , A. Mathieu
    •  & F. Sor
  8. Centro de Biologia Molecular, E-28049 Madrid, Spain;

    • J. P. G. Ballesta
    • , L. Franco
    • , J. Hoenicka
    • , A. Jimenez
    • , M. Remacha
    •  & E. Sanz
  9. Faculté des Sciences Agronomiques, B-5030 Gembloux. Belgium;

    • G. Berben
    • , P. A. Bolle
    • , V. Gilliquet
    •  & F. Hilger
  10. Dipartimento di Chimica Biologica. 1-35100 Padova, Italy;

    • E. Bergantino
    • , G. Carignani
    • , G Lanfranchi
    •  & G. Valle
  11. Institut de Génétique et Microbiologie, Université de Paris-Sud, 91405 Orsay, France;

    • M. Bolotin-Fukuhara
    • , B. Daignan-Fornier
    • , C. Doira
    • , M. C. Francingues-Gaillard
    • , F. Iborra
    • , M. Jacquet
    • , L. Mallet
    • , C. Pallier
    • , A. Raynal
    •  & C. Soustelle
  12. Department of Molecular and Cell Biology, University of Aberdeen, UK;

    • A. J. P. Brown
    • , A. B. Roberts
    •  & B. L. Wicksteed
  13. Service de Biochimie CEN Saclay, F-91191. France;

    • J. M. Buhler
  14. Institut für Mikrobiologie, D-6100 Darmstadt, Germany;

    • H. Cederberg
    • , S. Hohmann
    • , K. Huse
    • , I. Schaaff-Gerstenschlager
    •  & F. K. Zimmermann
  15. Lab. voor Moleculaire Biologie, B-9000 Gent, Belgium;

    • R. Contreras
    • , J. Demolder
    • , W. Fiers
    •  & F. Molemans
  16. Katholieke Universiteit Lab. voor Gentechnologie, B-3001 Leuven, Belgium;

    • E. Defoor
    • , M. Voet
    •  & G. Volckaert
  17. La Cruz del Campo S.A., PO Box 53. E-41080 Sevilla, Spain;

    • M. Delgado
    • , M. Esteban
    •  & L. Navas
  18. Research Institute of CERIA-COOVI. B-1070 Bruxelles, Belgium;

    • E. Dubois
    • , D. Gigot
    • , N. Glansdorff
    • , F. Messenguy
    • , A. Pierard
    •  & B. Scherens
  19. Institut Pasteur, F-75724 Paris Cedex 15, France;

    • B. Dujon
    • , C. Fairhead
    •  & A. Thierry
  20. Institut für Mikrobiologie und Molekularbiologie der Universität, 6300 Giessen, Germany;

    • A. Dusterhoft
    • , D. Erdmann
    • , J. Hegemann
    • , P. Philippsen
    • , B. Schweitzer
    • , R. Spiegelberg
    •  & S. Steiner
  21. Institut fur Physiologische Chemie. Universität München, 8000 München, Germany;

    • H. Feldmann
    • , G. Mannhaupet
    •  & I. Vetter
  22. University of Rome. Department of Cell and Developmental Biology. 1-00185 Roma, Italy;

    • L. Frontali
    • , P. Grisanti
    •  & C. Wilson
  23. AFRC Institute of Food Research, Norwich Research Park, Colney NR4 7UA, UK;

    • L. J. Fuller
    • , C Lewis
    • , R. A. McKee
    •  & B. M. Pearson
  24. Unité de Biochimie Physiologique. Université de Louvain, B-1348 Louvain-la-Neuve, Belgium;

    • A. Goffeau
    • , B. Purnelle
    •  & J. Skala
  25. Université Libre de Bruxelles, Lab. Cell Physiology and Yeast Genetics, B-1050 Bruxelles, Belgium;

    • M. Grenson
    • , J. C. Jauniaux
    •  & L. A. Urrestarazu
  26. University of Amsterdam, Section for Molecular Biology, NL-1098 SM Amsterdam, The Netherlands;

    • L. A. Grivell
    • , M. de Haan
    •  & M.J. Maat
  27. Martinsried Inst. for Protein Sequences, D-8033 Martinsried, Germany;

    • M. Haasemann
    • , P Kreisl
    • , H. W. Mewes
    • , B. Poetsch
    •  & J. G. Sgouros
  28. Génétique Moléculaire. École Normale Superieure, F-75005 Paris, France;

    • D. Hatat
    • , C. Jacq
    • , J. Perea
    •  & Y. Shu
  29. Centre de Génétique Moleculaire du CNRS, F-91190 Gif-sur-Yvette. France;

    • C. J. Herbert
    • , Y. Jia
    •  & P. P. Slonimski
  30. Institut für Mikrobiologie der Universität Dusseldorf, D-4000 Dusseldorf 1, Germany;

    • C. P. Hollenberg
    • , U. Kleinhans
    • , K Lutzenkirchen
    • , M. Ramezani Rad
    •  & G. Xu
  31. Department of Biology, Kobe University, Kobe 657. Japan;

    • K. Isono
    •  & A. Yoshikawa
  32. Trinity College, Department of Genetics, Dublin 2, Ireland;

    • A. Kelly
    • , D. McConnell
    • , M. A. Montague
    • , D. Noone
    •  & H. Wang
  33. Department of Biochemistry and Molecular Biology. Vrije Universiteit, NL-1081 HV Amsterdam, The Netherlands;

    • C. G. vanderLinden
    • , C. T. C. Maurer
    • , R. J. Planta
    • , J. C. van Vliet-Reedijk
    •  & P. Vreken
  34. Department of Microbiology and Molecular Genetics, New Jersey Medical School, NJ07103, USA;

    • C. S. Newlon
  35. Fakultät für Biologie der Universität, D-7750 Konstanz. Germany;

    • F. Pohl
    • , P. Richterich
    •  & H. Wurst
  36. Carlsberg Lab., DK-2500 Copenhagen Valby, Denmark;

    • S. W. Rasmussen
    •  & D. von Wettstein
  37. Commission of the European Communities. B-1049 Brussels, Belgium

    • A. Goffeau

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https://doi.org/10.1038/357038a0

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