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Presentation of viral antigen by MHC class I molecules is dependent on a putative peptide transporter heterodimer

Abstract

MAJOR histocompatibility complex (MHC) class I molecules present peptides derived from the endogenous protein pool to cytotoxic T lymphocytes, which can thus recognize intracellular antigen1–3. This pathway may depend on a transporter (PSF1) (refs 4–6) to mediate entry of the cytosolic peptides into a pre-Golgi compartment where they hind to class I heavy chains and promote their stable assembly with β2-microglobulin7–12. There is, however, only indirect support for this function of PSF1 (ref. 6). Here we show that PSF1 is necessary for the efficient assembly of class I molecules and enables them to present a peptide epitope derived from endogenously synthesized viral antigen. Immunochemical and genetic data demonstrate that the PSF1 poly peptide is associated with a complementary transporter chain, which is polymorphic and is encoded by the PSF2 gene13, which is closely linked to PSF1.

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Spies, T., Cerundolo, V., Colonna, M. et al. Presentation of viral antigen by MHC class I molecules is dependent on a putative peptide transporter heterodimer. Nature 355, 644–646 (1992). https://doi.org/10.1038/355644a0

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