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Structure and functional properties of human general transcription factor IIE

Nature volume 354, pages 369–373 (1991)Cite this article

Abstract

The general transcription factor HE (TFIIE) is an essential component of the eukaryotic RNA poly-merase II initiation complex. We have isolated human complementary DNA clones for both the subunits of TFIIE. Using purified recombinant proteins we find that both subunits are essential to form a stable preinitiation complex and to reconstitute basal-level and Sp1-activated transcription in vitro. Analysis of their predicted amino-acid sequences reveals several intriguing structural motifs that could provide insight into the role of TFIIE in transcription initiation.

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Author notes

  1. Juan Inostroza, Osvaldo Flores and Danny Reinberg: Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854-5635, USA

Authors and Affiliations

  1. Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California, 94720, USA

    Michael Gregory Peterson, Juan Inostroza, Mary E. Maxon, Osvaldo Flores, Arie Admon, Danny Reinberg & Robert Tjian

Authors
  1. Michael Gregory Peterson
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  2. Juan Inostroza
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  3. Mary E. Maxon
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  4. Osvaldo Flores
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  5. Arie Admon
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  6. Danny Reinberg
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  7. Robert Tjian
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Gregory Peterson, M., Inostroza, J., Maxon, M. et al. Structure and functional properties of human general transcription factor IIE. Nature 354, 369–373 (1991). https://doi.org/10.1038/354369a0

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