Abstract
The movement of microtubules on the kinetochores of isolated chromosomes has been examined by video microscopy. Two different microtubule-based motors on the kinetochore were identified, which have opposite directions of movement. The activities of these two motors can be regulated by factors that can influence phosphorylation.
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References
Rieder, C. L. Chromosoma 84, 145–158 (1981).
Roos, U. P. Chromosoma 41, 195–220 (1973).
Euteneuer, U., Jackson, W. T. & Mclntosh, J. R. J. Cell Biol. 94, 644–653 (1982).
Bajer, A. S. & Mole-Bajer, J. Int. Rev. Cytol. 3, 1–264 (1972).
Mitchison, T. J., Schultze, E., Evans, L. & Kirschner, M. W. Cell 45, 515–527 (1986).
Gorbsky, G. J., Sammak, P. J. & Borisy, G. G. J. Cell Biol. 104, 9–18 (1987).
Koshland, D., Mitchison, T. J. & Kirschner, M. W. Nature 311, 499–504 (1988).
Inoue, S., Fuseler, J., Salmon, E. D. & Ellis, G. W. Biophys. J. 15, 725–744 (1975).
Mitchison, T. J. & Kirschner, M. W. J. Cell Biol. 101, 755–765 (1985).
Vallee, R. B. & Shpetner, H. S. A. Rev. Biochem. 59, 909–932 (1990).
Rieder, C. L. & Alexander, S. P. J. Cell Biol. 110, 81–95 (1990).
Pfarr, C. M. et al. Nature 345, 263–265 (1990).
Steuer, E. R., Wordeman, L., Schroer, T. A. & Sheetz, M. P. Nature 345, 266–268 (1990).
Mitchison, T. J. & Kirschner, M. W. J. Cell Biol. 101, 767–777 (1985).
Hyman, A. A. & Mitchison, T. J. J. Cell Biol. 110, 1607–1616 (1990).
Mitchison, T. J. A. Rev. Cell biol. 4, 527–549 (1988).
Hyman, A. A. J. Cell Sci. (in the press).
Cyert, M. S. & Thorner, J. Cell 57, 891–893 (1989).
Goody, R. S., Eckstein, F. & Schirmer, R. H. Biochim. biophys. Res. Commun. 58, 155–161 (1972).
Chlebowski, J. F. & Coleman, J. E. J. biol. Chem. 247, 6007–6010 (1972).
Shimizu, T. et al. J. Cell Biol. 112, 1189–1199 (1991).
Walker, R. A., Salmon, E. D. & Endow, S. A. Nature 347, 780–782 (1990).
Vale, R. D., Reese, T. S. & Sheetz, M. P. Cell 42, 39–50 (1985).
Saxton, W. M., Hicks, J., Goldstein, L. S. B. & Raph, E. C. Cell (in the press).
Wright, B. D. et al. J. Cell Biol. (in the press).
Yang, J. T., Saxton, W. M. & Goldstein, L. S. B. Proc. natn. Acad Sci. U.S.A. 85, 1846–1886 (1988).
McDonald, H. B. & Goldstein, L. S. B. Cell 61, 991–1000 (1990).
Endow, S. A., Henikoff, S. & Niedziela, L. S. Nature 345, 81–83 (1990).
Cohn, S. A., Ingold, A. L. & Scholey, J. M. J. biol. Chem. 264, 4290–4297 (1989).
Verde, F., Labbe, J.-c., Doree, M. & Karsenti, E. Nature 343, 233–238 (1990).
Cyert, M. S., Scherson, T. & Kirschner, M. W. Devl Biol. 129, 209–216 (1988).
Wordeman, L. & Cande, W. Z. Cell 50 (1987).
Wordeman, L., Steuer, E., Sheetz, M. & Mitchison, T. J. J. Cell Biol. (in the press).
Dustin, P. Microtubules (Springer-Verlag, Berlin, 1984).
Begg, D. A. & Ellis, G. W. J. Cell Biol. 82, 528–541 (1979).
Rieder, C. L., Davison, E. A., Jensen, L. C. W., Cassimeris, L. & Salmon, E. D. J. Cell Biol. 103, 581–591 (1986).
Rozdzial, M. M. & Haimo, L. T. Cell 47, 1061–1070 (1986).
Mitchison, T. J. in Cell Movement Vol. 2 (eds Warner, F. D. & Mcintosh, J. R.) 421–430 (Liss, New York, 1988).
Hays, T. S. & Salmon, E. D. J. Cell Biol. 110, 391–404 (1990).
Salmon, E. D. In Cell Movement Vol. 2 (eds Warner, F. D. & Mcintosh, J. R.) 431–440 (Liss, New York, 1988).
Murray, A., Solomon, M. & Kirschner, M. Nature 339, 280–286 (1989).
Hyman, A. A. et al. Meth. Enzym. 196, 478–485 (1991).
Kishino, A. & Yanagida, T. Nature 334, 74–76 (1988).
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Hyman, A., Mitchison, T. Two different microtubule-based motor activities with opposite polarities in kinetochores. Nature 351, 206–211 (1991). https://doi.org/10.1038/351206a0
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DOI: https://doi.org/10.1038/351206a0
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