Abstract
Neuroglia are non-neuronal cells in the nervous system and are involved in virtually every aspect of neural function. Because of the ambiguity of glial function, the definition of glial cells relies chiefly on structural and biochemical characteristics. The use of molecular markers in identifying glial cells along their differentiation pathways is further complicated by recent findings that many of the molecules are also expressed by cells of the neuronal lineage. So, how specific are glial markers and how can a glial cell be defined during development?
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References
Skoff, R. P., Price, D. L. & Stocks, A. Electron microscopic autoradiographic studies of gliogenesis in rat optic nerve. II. Time of origin. J. Comp. Neurol. 169, 313–334 (1976).
Chandross, K. J. et al. Identification and characterization of early glial progenitors using a transgenic selection strategy. J. Neurosci. 19, 759–774 (1999).
Zhou, Q., Wang, S. & Anderson, D. J. Identification of a novel family of oligodendrocyte lineage-specific basic helix–loop–helix transcription factors. Neuron 25, 331–343 (2000).
Rao, M. S., Noble, M. & Mayer-Proschel, M. A tripotential glial precursor is present in the developing spinal cord. Proc. Natl Acad. Sci. USA 95, 3996–4001 (1998).
Rakic, P. Mode of cell migration to the superficial layers of fetal monkey neocortex. J. Comp. Neurol. 145, 61–83 (1972).
Voigt, T. Development of glial cells in the cerebral wall of ferrets: direct tracing of their transformation from radial glia into astrocytes. J. Comp. Neurol. 289, 74–88 (1989).
Malatesta, P., Hartfuss, E. & Gotz, M. Isolation of radial glial cells by fluorescent-activated cell sorting reveals a neuronal lineage. Development 127, 5253–5263 (2000).
Misson, J.-P., Edwards, M. A., Yamamoto, M. & Caviness, V. S. Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker. Brain Res. Dev. Brain Res. 44, 95–108 (1988).
Seidman, K. J., Teng, A. L., Rosenkopf, R., Spilotro, P. & Weyhenmeyer, J. A. Isolation, cloning and characterization of a putative type-1 astrocyte cell line. Brain Res. 753, 16–26 (1997).
Mi, H. & Barres, B. A. Purification and characterization of astrocyte precursor cells in the developing rat optic nerve. J. Neurosci. 19, 1049–1061 (1999).
Miller, R. H. & Ono, K. Morphological analysis of the early stages of oligodendrocyte development in the vertebrate central nervous system. Microsc. Res. Tech. 41, 441–453 (1998).
Sommer, I. & Schachner, M. Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system. Dev. Biol. 83, 311–327 (1981).
Schwarz, A. & Futerman, A. H. Determination of the localization of gangliosides using anti-ganglioside antibodies: comparison of fixation methods. J. Histochem. Cytochem. 45, 611–618 (1997).
McLendon, R. E. & Bigner, D. D. Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations. Brain Pathol. 4, 221–228 (1994).
Shamblott, M. J. et al. Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro. Proc. Natl Acad. Sci. USA 98, 113–118 (2001).
Zhang, S.-C., Wernig, M., Duncan, I. D., Brüstle, O. & Thomson, J. A. In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nature Biotechnol. (in the press).
Eng, L. F., Ghirnikar, R. S. & Lee, Y. L. Glial fibrillary acidic protein: GFAP-thirty-one years (1969–2000). Neurochem. Res. 25, 1439–1451 (2000).
Eisenbarth, G. S., Walsh, F. S. & Nirenberg, M. Monoclonal antibody to a plasma membrane antigen of neurons. Proc. Natl Acad. Sci. USA 76, 4913–4917 (1979).
Raff, M. C., Miller, R. H. & Noble, M. A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature 303, 390–396 (1983).
Satoh, J. & Kim, S. U. Ganglioside markers GD3, GD2, and A2B5 in fetal human neurons and glial cells in culture. Dev. Neurosci. 17, 137–148 (1995).
Zhang, S.-C., Ge, B. & Duncan, I. D. Tracing human oligodendroglial development in vitro. J. Neurosci. Res. 59, 421–429 (2000).
Flax. J. D. et al. Engraftable human neural stem cells respond to developmental cures, replace neurons, and express foreign genes. Nature Biotechnol. 16, 1033–1039 (1998).
Doetsch, F., Caille, I., Lim, D. A., Garcia-Verdugo, J. M. & Alvarez-Buylla, A. Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. Cell 97, 703–716 (1999).
Laywell, E. D., Rakic, P., Kukekov, V. G., Holland, E. C. & Steindler, D. A. Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain. Proc. Natl Acad. Sci. USA 97, 13883–13888 (2000).
Kondo, T. & Raff, M. Oligodendrocyte precursor cells reprogrammed to become multipotential CNS stem cells. Science 289, 1754–1757 (2000).
Williams, B. P., Read, J. & Price, J. The generation of neurons and oligodendrocytes from a common precursor cell. Neuron 7, 685–693 (1991).
Mizuguchi, R. et al. Combinatorial roles of Olig2 and neurogenin2 in the coordinated induction of pan-neuronal and subtype-specific properties of motoneurons. Neuron 31, 757–771 (2001).
Zhou, Q., Choi, G. & Anderson, D. J. The bHLH transcription factor Oligo2 promotes oligodendrocyte differentiation in collaboration with Nkx2.2. Neuron 31, 791–807 (2001).
Nishiyama, A., Chang, A. & Trapp, B. D. NG2+ glial cells: a novel glial cell population in the adult brain. J. Neuropathol. Exp. Neurol. 58, 1113–1124 (1999).
Geschwind, D. H. et al. A genetic analysis of neural progenitor differentiation. Neuron 29, 325–339 (2001).
Kornblum, H. I. & Geschwind, D. H. Molecular markers in CNS stem cell research: hitting a moving target. Nature Rev. Neurosci.. 2, 843–846 (2001).
Sidhu, S. S. Phage display in pharmaceutical biotechnology. Curr. Opin. Biotechnol. 11, 610–616 (2000).
Acknowledgements
I thank S. Fedoroff and A. Messing for constructive criticisms and suggestions, and B. Lyons for preparation of the figures.
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astrocyte-specific glutamate transporter
myelin-associated glycoprotein
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Zhang, SC. Defining glial cells during CNS development. Nat Rev Neurosci 2, 840–843 (2001). https://doi.org/10.1038/35097593
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DOI: https://doi.org/10.1038/35097593
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