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Matrix proteins can generate the higher order architecture of the Golgi apparatus

Nature volume 407, pages 1022–1026 (2000)Cite this article

Abstract

The Golgi apparatus in animal cells comprises a reticulum of linked stacks in the pericentriolar and often in the juxtanuclear regions of the cell1. The unique architecture of this organelle is thought to depend on the cytoskeleton2 and cytoplasmic matrix proteins3,4—the best characterized being the golgin family of fibrous, coiled-coil proteins and the GRASP family of stacking proteins5,6,7,8,9,10. Here we show that these matrix proteins can be separated from oligosaccharide-modifying enzymes in the Golgi stack without affecting their ability to form a ribbon-like reticulum in the correct location near to the nucleus. Our data suggest that the Golgi is a structural scaffold that can exist independently of, but is normally populated by, the enzyme-containing membranes that modify transiting cargo. This new concept of the Golgi further indicates that the Golgi may be an autonomous organelle rather than one that is in simple dynamic equilibrium with the endoplasmic reticulum.

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Figure 1: The effect of blocking ER exit by Sar1DN on reassembly of the Golgi apparatus after BFA wash out.
Figure 2: Ultrastructure of NRK cells treated with BFA and/or micro-injected with Sar1DN.
Figure 3: Localization of GM130 by immuno-electron microscopy.
Figure 4: Effect of Sar1DN on the pattern of Golgi enzymes and matrix proteins.

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Acknowledgements

We would like to thank J. Shorter for his comments. This work was funded by the NIH and J.S. was supported by a postdoctoral fellowship from the Deutsche Forschungsgemeinschaft.

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Authors and Affiliations

  1. Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, PO Box 208002 , New Haven, 06520-8002, Connecticut, USA

    Joachim Seemann, Marc Pypaert & Graham Warren

  2. Institute of Biotechnology, Electron Microscopy Unit, Biocenter 1, PO Box 56 (Viikinkaari 9), 00014

    Eija Jokitalo

  3. University of Helsinki, Finland

    Eija Jokitalo

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  1. Joachim Seemann
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Correspondence to Graham Warren.

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Seemann, J., Jokitalo, E., Pypaert, M. et al. Matrix proteins can generate the higher order architecture of the Golgi apparatus. Nature 407, 1022–1026 (2000). https://doi.org/10.1038/35039538

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