Abstract
THE tightly centromere-linked gene SPO15 is essential for meiotic cell division in the yeast Saccharomyces cerevisiae. Diploid cells without the intact SPO15 gene product are able to complete premeiotic DNA synthesis and genetic recombination, but are unable to traverse the division cycles. Electron microscopy of blocked cells reveals a duplicated but unseparated spindle-pole body. Thus cells are unable to form a bipolar spindle. Sequence analysis of SPO15 DNA reveals an open reading frame that predicts a protein of 704 amino acids. This protein is identical to VPS1, a gene involved in vacuolar protein sorting in yeast which has significant sequence homology (45% overall, 66% over 300 amino acids) to the microtubule bundling-protein, dynamin. The SPO15 gene product expressed in Escherichia coli can be affinity-purified with microtubules. SPO15 encodes a protein that is likely to be involved in a microtubule-dependent process required for the timely separation of spindle-pole bodies in meiosis
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Yeh, E., Carbon, J. & Bloom, K. Molec. Cell Biol. 6, 158–167 (1986).
Rothman, J. H. et al. Cell 61, 1063–1074 (1990).
Rothman, J. H. & Stevens, T. H. Cell 47, 1041–1051 (1986).
Obar, R. A. et al. Nature 347, 256–261 (1990).
Jacobs, C. W., Adams, A. E., Szaniszlo, P. J. & Pringle, J. R. J. Cell Biol. 107, 1409–1426 (1988).
Tabor, S. in Current Protocols in Molecular Biology, (eds Ausubel, F. A. et al.) 16.2.1–16.2.11 (Greene and Wiley-Interscience, New York, 1990).
Shpetner, H. S. & Vallee, R. B. Cell 59, 421–432 (1989).
Hall, A. Science 249, 635–640 (1990).
Meluh, P. & Rose, M. D. Cell 60, 1029–1041 (1990).
Enos, A. P. & Morris, N. R. Cell 60, 1019–1027 (1990).
Byers, B. & Goetsch, L. J. Bact. 124, 511–523 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yeh, E., Driscoll, R., Coltrera, M. et al. A dynamin-like protein encoded by the yeast sporulation gene SP015. Nature 349, 713–715 (1991). https://doi.org/10.1038/349713a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/349713a0
This article is cited by
-
Yeast motor proteins
Folia Microbiologica (1995)
-
Identification and characterization of CEN12 in the budding yeast Saccharomyces cerevisiae
Current Genetics (1995)
-
Normal mitochondrial structure and genome maintenance in yeast requires the dynamin-like product of the MGM1 gene
Current Genetics (1993)
-
Dynamin is a GTPase stimulated to high levels of activity by microtubules
Nature (1992)
-
Molecular motors in axonal transport
Molecular Neurobiology (1992)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.