Abstract
THE protein kinase MAP kinase, also called MAP2 kinase, is a serine/threonine kinase whose activation and phosphorylation are induced by a variety of mitogens1–6, and which is thought to have a critical role in a network of protein kinases in mitogenic signal transduction1–7. A burst in kinase activation8,9and protein phosphorylation10 may also be important in triggering the dramatic reorganization of the cell during the transition from interphase to mitosis. The interphase–metaphase transition of microtubule arrays is under the control of p34cdc2 kinase11, a central control element in the G2–M transition of the cell cycle12. Here we show that a Xenopus kinase, closely related to the mitogen-activated mammalian MAP kinase, is phosphorylated and activated during M phase of meiotic and mitotic cell cycles, and that the interphase–metaphase transition of microtubule arrays can be induced by the addition of purified Xenopus M phase-activated MAP kinase or mammalian mitogen-activated MAP kinase to interphase extracts in vitro.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ray, L. B. & Sturgill, T. W. Proc. natn. Acad. Sci. U.S.A. 84, 1502–1506 (1987).
Hoshi, M., Nishida, E. & Sakai, H. J. biol. Chem. 263, 5396–5401 (1988).
Ray, L. B. & Sturgill, T. W. J. biol. Chem. 263, 12721–12727 (1988).
Hoshi, M., Nishida, E. & Sakai, H. Eur. J. Biochem. 184, 477–486 (1989).
Rossomando, A. J., Payne, D. M., Weber, M. J. & Sturgill, T. W. Proc. natn. Acad. Sci. U.S.A. 86, 6940–6943 (1989).
Gotoh, Y., Nishida, E. & Sakai, H. Eur. J. Biochem. 193, 671–674 (1990).
Sturgill, T. W., Ray, L. B., Erikson, E. & Maller, J. L. Nature 334, 715–718 (1988).
Cicirelli, M. F., Pelech, S. L. & Krebs, E. G. J. biol. Chem. 263, 2009–2019 (1988).
Sanghera, J. S., Paddon, H. B., Bader, S. A. & Pelech, S. L. J. biol. Chem. 265, 52–57 (1990).
Maller, J. L., Wu, M. & Gerhart, J. C. Devl Biol. 58, 295–312 (1977).
Verde, F., Labbe, J.-C., Doree, M. & Karsenti, E. Nature 343, 233–238 (1990).
Nurse, P. Nature 344, 503–508 (1990).
Anderson, N. G., Mailer, J. L., Tonks, N. K. & Sturgill, T. W. Nature 343, 651–653 (1990).
Boulton, T. G. et al. Science 249, 64–67 (1990).
Cooper, J. A. Molec. cell. Biol. 9, 3143–3147 (1989).
Gard, D. L. & Kirschner, M. W. J. Cell Biol. 105, 2191–2201 (1987).
Erikson, E. & Maller, J. L. J. biol. Chem. 264, 13711–13717 (1989).
Kase, H. et al. Biochem. biophys. Res. Commun. 142, 436–440 (1987).
Tamaoki, T. et al. Biochem. biophys. Res. Commun. 135, 397–402 (1986).
Gotoh, Y. et al. Eur. J. Biochem. 193, 661–669 (1990).
Kameshita, I. & Fujisawa, H. Analyt. Biochem. 183, 139–143 (1989).
Stith, B. J. & Maller, J. L. Devl Biol. 102, 79–89 (1984).
Karsenti, E., Newport, J., Hubble, R. & Kirschner, M. J. Cell Biol. 98, 1730–1745 (1984).
Lohka, M. J. & Maller, J. L. J. Cell Biol. 101, 518–523 (1985).
Felix, M.-A., Pines, J., Hunt, T. & Karsenti, E. EMB0 J. 8, 3059–3069 (1989).
Mitchison, T. & Kirschner, M. Nature 312, 232–237 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gotoh, Y., Nishida, E., Matsuda, S. et al. In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase. Nature 349, 251–254 (1991). https://doi.org/10.1038/349251a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/349251a0
This article is cited by
-
AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division
Communications Biology (2021)
-
Mitofusin-2 is required for mouse oocyte meiotic maturation
Scientific Reports (2016)
-
Ras regulates kinesin 13 family members to control cell migration pathways in transformed human bronchial epithelial cells
Oncogene (2014)
-
Conservation of MAP kinase activity and MSP genes in parthenogenetic nematodes
BMC Developmental Biology (2010)
-
The roles of MAPKs in disease
Cell Research (2008)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.